Abstract
The application of copper (Cu)-based fungicides for crop protection plans has led to a high accumulation of Cu in soils, especially in vineyards. Copper is indeed an essential micronutrient for plants, but relatively high concentrations in soil or other growth substrates may cause toxicity phenomena, such as alteration of the plant’s growth and disturbance in the acquisition of mineral nutrients. This last aspect might be particularly relevant in the case of nitrate (NO3−) , whose acquisition in plants is finely regulated through the transcriptional regulation of NO3− transporters and plasma membrane H + -ATPase in response to the available concentration of the nutrient. In this study, cucumber plants were grown hydroponically and exposed to increasing concentrations of Cu ( i.e. , 0.2, 5, 20, 30, and 50 µM) to investigate their ability to respond to and acquire NO3− . To this end, the kinetics of substrate uptake and the transcriptional modulation of the molecular entities involved in the process have been assessed. Results showed that the inducibility of the high-affinity transport system was significantly affected by increasing Cu concentrations; at Cu levels higher than 20 µM, plants demonstrated either strongly reduced or abolished NO3− uptake activity. Nevertheless, the transcriptional modulation of both the nitrate transporter CsNRT2.1 and the accessory protein CsNRT3.1 was not coherent with the hindered NO3− uptake activity. On the contrary, CsHA2 was downregulated, thus suggesting that a possible impairment in the generation of the proton gradient across the root PM could be the cause of the abolishment of NO3− uptake.
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Claims 6
Claim · model-written summary
Increasing copper significantly affected inducibility of the high-affinity nitrate transport system.
- Taxon
- cucumber
Cucumis sativuse1 - Treatment
- increasing Cu concentrationse0
- Outcome
- inducibility of the high-affinity transport systeme0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the inducibility of the high-affinity transport system was significantly affected by increasing Cu concentrations”
e1“cucumber plants were grown hydroponically”
Claim · model-written summary
Copper above 20 µM strongly reduced or abolished cucumber nitrate uptake.
- Taxon
- cucumberCucumis sativuse1
- Treatment
- Cu levels higher than 20 µMe0
- Outcome
- NO3− uptake activitye0
- Direction
- strongly reduced or abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at Cu levels higher than 20 µM, plants demonstrated either strongly reduced or abolished NO3− uptake activity”
e1“cucumber plants were grown hydroponically”
Claim · model-written summary
CsNRT2.1 transcriptional modulation did not align with impaired nitrate uptake.
- Taxon
- cucumberCucumis sativuse1
- Gene
- CsNRT2.1e0
- Outcome
- hindered NO3− uptake activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcriptional modulation of both the nitrate transporter CsNRT2.1 and the accessory protein CsNRT3.1 was not coherent with the hindered NO3− uptake activity”
e1“cucumber plants were grown hydroponically”
Claim · model-written summary
CsNRT3.1 transcriptional modulation did not align with impaired nitrate uptake.
- Taxon
- cucumberCucumis sativuse1
- Gene
- CsNRT3.1e0
- Outcome
- hindered NO3− uptake activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcriptional modulation of both the nitrate transporter CsNRT2.1 and the accessory protein CsNRT3.1 was not coherent with the hindered NO3− uptake activity”
e1“cucumber plants were grown hydroponically”
Claim · model-written summary
CsHA2 was downregulated under copper exposure.
- Taxon
- cucumberCucumis sativuse1
- Treatment
- increasing concentrations of Cue1
- Gene
- CsHA2e0
- Outcome
- CsHA2 was downregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsHA2 was downregulated”
e1“cucumber plants were grown hydroponically and exposed to increasing concentrations of Cu”
Claim · model-written summary
The authors suggest impaired root proton-gradient generation may cause loss of nitrate uptake.
- Taxon
- cucumberCucumis sativuse1
- Outcome
- abolishment of NO3− uptakee0
- Qualifier
- impairment in the generation of the proton gradient across the root PMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a possible impairment in the generation of the proton gradient across the root PM could be the cause of the abolishment of NO3− uptake”
e1“cucumber plants were grown hydroponically”
The same questions in other papers
copper atom · nutrient: content · Cucumis sativus (cucumber)Results agree
5 papers in the same direction as this paper
- negative
- negative
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.