Paper · 2017 · IOSR Journal of Biotechnology and Biochemistry

In vitro propagation of the endangered date palm (Phoenix dactylifera L.) cv. Ghazal: 2- Improvement of germination and development of embryos by silver nitrate and silver thiosulfate

Mohamed Ismail Diab

11
claims
18
verbatim quotes
7
citations
3
research areas
✓
Open access

Abstract

palm (Phoenix dactylifera L.) is one of the most importan tree crops in the deserts which is a tree high economic and commercial values. This study was performed to micro propagate of date palm cv. Ghazal via somatic embryogenesis. Although, it was conducted to examine different concentration of silver nitrate (SN) and silver thiosulfate (STS) for their effect on somatic embryogenesis development of date palm. In this context, the obtained results indicated that, all silver treatments, whether nitrate or thiosulfate, had a positive effect. The highest number, length and growth value of somatic embryos formed are obtained from embryogenic celli cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg/l SN or 1.0 ml/l STS. Moreover, the results have shown that the SN at 4.0 mg/l or 2.0 ml/l promoted the growth, proliferation, maturation and germination of somatic embryos of date palm. 80 and 82.2% of embryos had differentiated and germinated to plantlets when re-cultured on the same previously medium. While, the medium supplied with fullstrength MS medium with 1.0 mg/l IBA and 1.0 mg/l SN or 0.5 ml/l STS showed superiority in the number of roots and their length in comparison with the other treatments. High frequencies of survival rates in greenhouse conditions were obtained after acclimatized and transferred to pots containing a mixture of peat moss and perlite at equal volume. Accordingly, these results suggest that adding silver nitrate or silver thiosulfate to the culture medium of date palm cv.Ghazal may be beneficial to improve the somatic embryogenesis growth performance and their developed for this cultivar.

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Claims 11

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Silver nitrate and silver thiosulfate treatments had a positive effect on date palm somatic embryogenesis.

Taxon
date palmPhoenix dactyliferae0
Treatment
all silver treatmentse0
Outcome
somatic embryogenesis developmente0
Direction
positive effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“somatic embryogenesis development of date palm. In this context, the obtained results indicated that, all silver treatments, whether nitrate or thiosulfate, had a positive effect.”

✓ located verbatim in the source · abstract, characters 366–545 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Medium supplemented with 2.0 mg/l SN produced the highest somatic embryo number, length and growth value.

Taxon
date palmPhoenix dactyliferat0
Organ / stage
somatic embryose0
Treatment
2.0 mg/l SNe0
Outcome
number, length and growth value of somatic embryose0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest number, length and growth value of somatic embryos formed are obtained from embryogenic celli cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg/l SN or 1.0 ml/l STS.”

✓ located verbatim in the source · abstract, characters 546–742 · show in text ↑
t0

“date palm”

✓ located verbatim in the source · title, characters 39–48 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Medium supplemented with 1.0 ml/l STS produced the highest somatic embryo number, length and growth value.

Taxon
date palmPhoenix dactyliferat0
Organ / stage
somatic embryose0
Treatment
1.0 ml/l STSe0
Outcome
number, length and growth value of somatic embryose0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest number, length and growth value of somatic embryos formed are obtained from embryogenic celli cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg/l SN or 1.0 ml/l STS.”

✓ located verbatim in the source · abstract, characters 546–742 · show in text ↑
t0

“date palm”

✓ located verbatim in the source · title, characters 39–48 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

SN at 4.0 mg/l promoted date palm somatic embryo growth, proliferation, maturation and germination.

Taxon
date palmPhoenix dactyliferae0
Treatment
SN at 4.0 mg/le0
Outcome
growth, proliferation, maturation and germination of somatic embryose0
Direction
promotede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“SN at 4.0 mg/l or 2.0 ml/l promoted the growth, proliferation, maturation and germination of somatic embryos of date palm.”

✓ located verbatim in the source · abstract, characters 785–907 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A treatment at 2.0 ml/l promoted date palm somatic embryo growth, proliferation, maturation and germination.

Taxon
date palmPhoenix dactyliferae0
Treatment
2.0 ml/le0
Outcome
growth, proliferation, maturation and germination of somatic embryose0
Direction
promotede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“SN at 4.0 mg/l or 2.0 ml/l promoted the growth, proliferation, maturation and germination of somatic embryos of date palm.”

✓ located verbatim in the source · abstract, characters 785–907 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

After reculture, 80 of the date palm embryos differentiated and germinated to plantlets.

Taxon
date palmPhoenix dactyliferat0
Outcome
embryos had differentiated and germinated to plantletse0
Value
80e0
Qualifier
when re-cultured on the same previously mediume0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“80 and 82.2% of embryos had differentiated and germinated to plantlets when re-cultured on the same previously medium.”

✓ located verbatim in the source · abstract, characters 908–1026 · show in text ↑
t0

“date palm”

✓ located verbatim in the source · title, characters 39–48 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

After reculture, 82.2% of date palm embryos differentiated and germinated to plantlets.

Taxon
date palmPhoenix dactyliferat0
Outcome
embryos had differentiated and germinated to plantletse0
Value
82.2%e0
Qualifier
when re-cultured on the same previously mediume0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“80 and 82.2% of embryos had differentiated and germinated to plantlets when re-cultured on the same previously medium.”

✓ located verbatim in the source · abstract, characters 908–1026 · show in text ↑
t0

“date palm”

✓ located verbatim in the source · title, characters 39–48 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Full-strength MS with IBA and SN gave superior date palm root number and length.

Taxon
date palmPhoenix dactyliferat0
Treatment
fullstrength MS medium with 1.0 mg/l IBA and 1.0 mg/l SNe0
Outcome
number of roots and their lengthe0
Direction
superioritye0
Comparator
other treatmentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“fullstrength MS medium with 1.0 mg/l IBA and 1.0 mg/l SN or 0.5 ml/l STS showed superiority in the number of roots and their length in comparison with the other treatments.”

✓ located verbatim in the source · abstract, characters 1059–1231 · show in text ↑
t0

“date palm”

✓ located verbatim in the source · title, characters 39–48 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Full-strength MS with STS gave superior date palm root number and length.

Taxon
date palmPhoenix dactyliferat0
Treatment
0.5 ml/l STSe0
Outcome
number of roots and their lengthe0
Direction
superioritye0
Comparator
other treatmentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“fullstrength MS medium with 1.0 mg/l IBA and 1.0 mg/l SN or 0.5 ml/l STS showed superiority in the number of roots and their length in comparison with the other treatments.”

✓ located verbatim in the source · abstract, characters 1059–1231 · show in text ↑
t0

“date palm”

✓ located verbatim in the source · title, characters 39–48 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Date palm plantlets had high survival after greenhouse acclimatization in peat moss and perlite.

Taxon
date palmPhoenix dactyliferat0
Outcome
survival ratese0
Direction
High frequenciese0
Qualifier
mixture of peat moss and perlite at equal volumee0
Setting
greenhouse conditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“High frequencies of survival rates in greenhouse conditions were obtained after acclimatized and transferred to pots containing a mixture of peat moss and perlite at equal volume.”

✓ located verbatim in the source · abstract, characters 1232–1411 · show in text ↑
t0

“date palm”

✓ located verbatim in the source · title, characters 39–48 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors suggest silver nitrate or silver thiosulfate additions may improve somatic embryogenesis in Ghazal date palm.

Taxon
date palmPhoenix dactyliferae0
Material
cv.Ghazale0
Outcome
improve the somatic embryogenesis growth performancee0
Qualifier
may be beneficiale0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“adding silver nitrate or silver thiosulfate to the culture medium of date palm cv.Ghazal may be beneficial to improve the somatic embryogenesis growth performance and their developed for this cultivar.”

✓ located verbatim in the source · abstract, characters 1452–1653 · show in text ↑

indole-3-butyric acid · growth · Phoenix dactylifera (date palm)Positive and negative results

24 papers: 23 positive, 1 negative or no effect

22 papers in the same direction as this paper · 1 reporting no difference from an alternative

  1. no effect
  2. positive
  3. positive

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silver atom · growth · Phoenix dactylifera (date palm)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
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silver(1+) nitrate · growth · Phoenix dactylifera (date palm)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
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in vitro · growth · Phoenix dactylifera (date palm)Positive and negative results

51 papers: 50 positive, 1 negative or no effect

49 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

47 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.