Abstract
Abstract Drought stress caused by water deficit reduces plant productivity in many regions of the world. In plants, basic helix–loop–helix (bHLH) transcription factors regulate a wide range of cellular activities related to growth, development and stress response; however, the role of tomato SlbHLHs in drought stress responses remains elusive. Here, we used reverse genetics approaches to reveal the function of SlbHLH96, which is induced by drought and abscisic acid (ABA) treatment. We found that SlbHLH96 functions as a positive regulator of drought tolerance in tomato. Overexpression of SlbHLH96 in tomato improves drought tolerance by stimulating the expression of genes encoding antioxidants, ABA signaling molecules and stress-related proteins. In contrast, silencing of SlbHLH96 in tomato reduces drought tolerance. SlbHLH96 physically interacts with an ethylene-responsive factor, SlERF4, and silencing of SlERF4 in tomato also decreases drought tolerance. Furthermore, SlbHLH96 can repress the expression of the ABA catabolic gene, SlCYP707A2, through direct binding to its promoter. Our results uncover a novel mechanism of SlbHLH96-mediated drought tolerance in tomato plants, which can be exploited for breeding drought-resilient crops.
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Claims 7
Claim · model-written summary
Drought and ABA induced SlbHLH96.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- drought and abscisic acid (ABA) treatmente0
- Gene
- SlbHLH96e0
- Outcome
- SlbHLH96e0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlbHLH96, which is induced by drought and abscisic acid (ABA) treatment”
t0“tomato”
Claim · model-written summary
SlbHLH96 overexpression improved drought tolerance.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Overexpression of SlbHLH96e0
- Gene
- SlbHLH96e0
- Outcome
- drought tolerancee0
- Direction
- improvese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of SlbHLH96 in tomato improves drought tolerance”
Claim · model-written summary
SlbHLH96 overexpression stimulated antioxidant, ABA-signalling and stress protein gene expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlbHLH96t0
- Outcome
- expression of genes encoding antioxidants, ABA signaling molecules and stress-related proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stimulating the expression of genes encoding antioxidants, ABA signaling molecules and stress-related proteins”
t0“SlbHLH96, promotes drought tolerance in tomato”
Claim · model-written summary
SlbHLH96 silencing reduced drought tolerance.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- silencing of SlbHLH96e0
- Gene
- SlbHLH96e0
- Outcome
- drought tolerancee0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of SlbHLH96 in tomato reduces drought tolerance”
Claim · model-written summary
SlbHLH96 physically interacted with SlERF4.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlbHLH96e0
- Outcome
- physically interactse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlbHLH96 physically interacts with an ethylene-responsive factor, SlERF4”
t0“tomato”
Claim · model-written summary
Silencing SlERF4 decreased drought tolerance.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- silencing of SlERF4e0
- Gene
- SlERF4e0
- Outcome
- drought tolerancee0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of SlERF4 in tomato also decreases drought tolerance”
Claim · model-written summary
SlbHLH96 directly bound the SlCYP707A2 promoter and repressed expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlbHLH96e0
- Outcome
- expression of the ABA catabolic gene, SlCYP707A2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlbHLH96 can repress the expression of the ABA catabolic gene, SlCYP707A2, through direct binding to its promoter”
t0“tomato”
The same questions in other papers
loss of function · tolerance: abiotic · Solanum lycopersicum (tomato)Positive and negative results
40 papers in the same direction as this paper · 12 in the opposite direction
- positive
- negative
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See all 53 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.