Abstract
Background: Crown root is the main part of root system, which performs an important role in rice growth and development, especially in nutrition and water assimilation. Previously, we reported negative feedback regulation loop between Oryza sativa DNA BINDING WITH ONE FINGER 11 (OsDOF11) and cytokinin by Oryza sativa CYTOKININ OXIDASE/DEHYDROGENASE 4 (OsCKX4) in rice development. Methods: Reverse transcription quantitative RT-PCR analyses was used to analyze the related gene transcript level. Nitrogen and hormone were measured by CHN-Nitrogen analyser and Liquid chromatography mass spectrometer, respectively. Exogenous application of cytokinin and [13C] sucrose labeled stable isotope uptake experiments help us to explain the relationship between OsDOF11 and cytokinin. Results: We demonstrate the role of OsDOF11 in root development. We note that the loss function of OsDOF11 displays the reduced crown roots number, low activity of nitrogen assimilation and low content of cytokinin and auxin. The expression level of WUSCHEL-related homeobox (OsWOX11), A-type response regulator 2 (OsRR2), OsRR3, and OsCKX4 were decreased in osdof11-1, as well as in OsDOF11 RNA interference 9 mutants (RNAi-9 lines). Through Exogenous application of multiple concentrations of cytokinin as treatment to osdof11-1 mutant, RNAi-9 lines, and wild type (WT). We found that the crown roots number of osdof11-1 plants were rescued as the cytokinin concentration increased gradually from 1 μM to 10 μM, but the effect was weaker in RNAi-9 line. And cytokinin inhibited sucrose uptake activity from Murashige-Skoog medium with 3.0% sucrose (MS30) by OsDOF11 in rice root. Conclusions: OsDOF11 promotes crown root formation via cytokinin in oryza sativa. These results provide a physiological basis for further analysis of the OsDOF11 function of in rice root development.
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Claims 10
Claim · model-written summary
Loss of OsDOF11 reduced crown root number.
- Taxon
- rice
Oryza sativae1 - Gene
- OsDOF11e0
- Outcome
- crown roots numbere0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the loss function of OsDOF11 displays the reduced crown roots number”
e1“OsDOF11 in rice root.”
Claim · model-written summary
Loss of OsDOF11 reduced nitrogen assimilation activity.
- Taxon
- riceOryza sativae1
- Gene
- OsDOF11e1
- Outcome
- activity of nitrogen assimilatione0
- Direction
- lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low activity of nitrogen assimilation”
e1“OsDOF11 in rice root.”
Claim · model-written summary
Loss of OsDOF11 lowered cytokinin and auxin content.
- Taxon
- riceOryza sativae1
- Gene
- OsDOF11e1
- Outcome
- content of cytokinin and auxine0
- Direction
- lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low content of cytokinin and auxin”
e1“OsDOF11 in rice root.”
Claim · model-written summary
OsWOX11 expression decreased in osdof11-1 and RNAi-9.
- Taxon
- riceOryza sativae1
- Material
- osdof11-1e0
- Gene
- OsWOX11e0
- Outcome
- expression levele0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression level of WUSCHEL-related homeobox (OsWOX11), A-type response regulator 2 (OsRR2), OsRR3, and OsCKX4 were decreased in osdof11-1, as well as in OsDOF11 RNA interference 9 mutants (RNAi-9 lines)”
e1“OsDOF11 in rice root.”
Claim · model-written summary
OsRR2 expression decreased in osdof11-1 and RNAi-9.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression level of WUSCHEL-related homeobox (OsWOX11), A-type response regulator 2 (OsRR2), OsRR3, and OsCKX4 were decreased in osdof11-1, as well as in OsDOF11 RNA interference 9 mutants (RNAi-9 lines)”
e1“OsDOF11 in rice root.”
Claim · model-written summary
OsRR3 expression decreased in osdof11-1 and RNAi-9.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression level of WUSCHEL-related homeobox (OsWOX11), A-type response regulator 2 (OsRR2), OsRR3, and OsCKX4 were decreased in osdof11-1, as well as in OsDOF11 RNA interference 9 mutants (RNAi-9 lines)”
e1“OsDOF11 in rice root.”
Claim · model-written summary
OsCKX4 expression decreased in osdof11-1 and RNAi-9.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression level of WUSCHEL-related homeobox (OsWOX11), A-type response regulator 2 (OsRR2), OsRR3, and OsCKX4 were decreased in osdof11-1, as well as in OsDOF11 RNA interference 9 mutants (RNAi-9 lines)”
e1“OsDOF11 in rice root.”
Claim · model-written summary
Cytokinin restored osdof11-1 crown root number as concentration rose from 1 to 10 μM.
- Taxon
- riceOryza sativae1
- Material
- osdof11-1e0
- Treatment
- cytokinine0
- Outcome
- crown roots numbere0
- Direction
- rescuede0
- Value
- 10 μMe0
- Comparator
- 1 μMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“crown roots number of osdof11-1 plants were rescued as the cytokinin concentration increased gradually from 1 μM to 10 μM”
e1“OsDOF11 in rice root.”
Claim · model-written summary
Cytokinin inhibited sucrose uptake activity in rice roots.
- Taxon
- riceOryza sativae0
- Organ / stage
- roote0
- Treatment
- cytokinine0
- Gene
- OsDOF11e0
- Outcome
- sucrose uptake activitye0
- Direction
- inhibitede0
- Comparator
- Murashige-Skoog medium with 3.0% sucrose (MS30)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cytokinin inhibited sucrose uptake activity from Murashige-Skoog medium with 3.0% sucrose (MS30) by OsDOF11 in rice root”
Claim · model-written summary
The authors conclude OsDOF11 promotes crown root formation via cytokinin.
- Taxon
- oryza sativaOryza sativae0
- Gene
- OsDOF11e0
- Compound
- cytokinine0
- Outcome
- crown root formatione0
- Direction
- promotese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsDOF11 promotes crown root formation via cytokinin in oryza sativa”
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