Abstract
The phytohormones auxins and brassinosteroids are both essential regulators of physiological and developmental processes, and it has been suggested that they act inter-dependently and synergistically. In rice (Oryza sativa), auxin co-application improves the brassinosteroid response in the rice lamina inclination bioassay. Here, we showed that auxins stimulate brassinosteroid perception by regulating the level of brassinosteroid receptor. Auxin treatment increased expression of the rice brassinosteroid receptor gene OsBRI1. The promoter of OsBRI1 contains an auxin-response element (AuxRE) that is targeted by auxin-response factor (ARF) transcription factors. An AuxRE mutation abolished the induction of OsBRI1 expression by auxins, and OsBRI1 expression was down-regulated in an arf mutant. The AuxRE motif in the OsBRI1 promoter, and thus the transient up-regulation of OsBRI1 expression caused by treatment with indole-3-acetic acid, is essential for the indole-3-acetic acid-induced increase in sensitivity to brassinosteroids. These findings demonstrate that some ARFs control the degree of brassinosteroid perception required for normal growth and development in rice. Although multi-level interactions between auxins and brassinosteroids have previously been reported, our findings suggest a mechanism by which auxins control cellular sensitivity to brassinosteroids, and further support the notion that interactions between auxins and brassinosteroids are extensive and complex.
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Claims 8
Claim · model-written summary
Auxin co-application improved the brassinosteroid response in rice lamina inclination.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- laminae0
- Treatment
- auxin co-applicatione0
- Outcome
- brassinosteroid responsee0
- Direction
- improvese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In rice (Oryza sativa), auxin co-application improves the brassinosteroid response in the rice lamina inclination bioassay.”
Claim · model-written summary
Auxins stimulated brassinosteroid perception by regulating receptor level.
- Taxon
- riceOryza sativat0
- Compound
- auxinse0
- Outcome
- brassinosteroid perceptione0
- Direction
- stimulatee0
- Qualifier
- regulating the level of brassinosteroid receptore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“auxins stimulate brassinosteroid perception by regulating the level of brassinosteroid receptor”
t0“rice”
Claim · model-written summary
Auxin treatment increased expression of OsBRI1.
- Taxon
- riceOryza sativae0
- Treatment
- Auxin treatmente0
- Gene
- OsBRI1e0
- Outcome
- expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Auxin treatment increased expression of the rice brassinosteroid receptor gene OsBRI1.”
Claim · model-written summary
The OsBRI1 promoter contained an AuxRE targeted by ARF transcription factors.
- Taxon
- riceOryza sativat0
- Gene
- OsBRI1e0
- Outcome
- contains an auxin-response element (AuxRE)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The promoter of OsBRI1 contains an auxin-response element (AuxRE) that is targeted by auxin-response factor (ARF) transcription factors.”
t0“rice”
Claim · model-written summary
AuxRE mutation abolished auxin induction of OsBRI1 expression.
- Taxon
- riceOryza sativat0
- Treatment
- AuxRE mutatione0
- Gene
- OsBRI1e0
- Outcome
- induction of OsBRI1 expressione0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An AuxRE mutation abolished the induction of OsBRI1 expression by auxins”
t0“rice”
Claim · model-written summary
OsBRI1 expression was down-regulated in an arf mutant.
- Taxon
- riceOryza sativat0
- Material
- arf mutante0
- Gene
- OsBRI1e0
- Outcome
- OsBRI1 expressione0
- Direction
- down-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsBRI1 expression was down-regulated in an arf mutant”
t0“rice”
Claim · model-written summary
The AuxRE motif and transient OsBRI1 up-regulation were essential for IAA-induced brassinosteroid sensitivity.
- Taxon
- riceOryza sativat0
- Treatment
- indole-3-acetic acide0
- Gene
- OsBRI1e0
- Outcome
- increase in sensitivity to brassinosteroidse0
- Qualifier
- AuxRE motife0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The AuxRE motif in the OsBRI1 promoter, and thus the transient up-regulation of OsBRI1 expression caused by treatment with indole-3-acetic acid, is essential for the indole-3-acetic acid-induced increase in sensitivity to brassinosteroids.”
t0“rice”
Claim · model-written summary
Some ARFs controlled the level of brassinosteroid perception needed for normal rice development.
- Taxon
- riceOryza sativae0
- Gene
- ARFse0
- Outcome
- degree of brassinosteroid perceptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some ARFs control the degree of brassinosteroid perception required for normal growth and development in rice”
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See all 45 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.