Abstract
The phytohormone salicylic acid (SA) plays a crucial role in plant growth and development. However, the mechanism of high-concentration SA-affected gravitropic response in plant root growth and root hair development is still largely unclear. In this study, wild-type, pin2 mutant and various transgenic fluorescence marker lines of Arabidopsis thaliana were investigated to understand how root growth is affected by high SA treatment under gravitropic stress conditions. We found that exogenous SA application inhibited gravitropic root growth and root hair development in a dose-dependent manner. Further analyses using DIRECT REPEAT5 (DR5)-GFP, auxin sensor DII-VENUS, auxin efflux transporter PIN2-GFP, trans-Golgi network/early endosome (TGN/EE) clathrin-light-chain 2 (CLC2)-mCherry and prevacuolar compartment (PVC) (Rha1)-mCherry transgenic marker lines demonstrated that high SA treatment severely affected auxin accumulation, root-specific PIN2 distribution and PIN2 gene transcription and promoted the vacuolar degradation of PIN2, possibly independent of clathrin-mediated endocytic protein trafficking. Our findings proposed a new underlying mechanism of SA-affected gravitropic root growth and root hair development via the regulation of PIN2 gene transcription and PIN2 protein endocytosis in plants.
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Claims 4
Claim · model-written summary
Exogenous salicylic acid inhibited gravitropic root growth in a dose-dependent manner.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Organ / stage
- roote1
- Treatment
- exogenous SA applicatione1
- Outcome
- gravitropic root growthe1
- Direction
- inhibitede1
- Qualifier
- in a dose-dependent mannere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e1“We found that exogenous SA application inhibited gravitropic root growth and root hair development in a dose-dependent manner”
Claim · model-written summary
High SA treatment severely affected auxin accumulation, root-specific PIN2 distribution and PIN2 gene transcription.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- high SA treatmente2
- Gene
- PIN2e2
- Outcome
- auxin accumulation, root-specific PIN2 distribution and PIN2 gene transcriptione2
- Direction
- severely affectede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e2“high SA treatment severely affected auxin accumulation, root-specific PIN2 distribution and PIN2 gene transcription”
Claim · model-written summary
High SA treatment promoted vacuolar degradation of PIN2, possibly independent of clathrin-mediated endocytic trafficking.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- high SA treatmente2
- Gene
- PIN2e3
- Outcome
- vacuolar degradation of PIN2e3
- Direction
- promotede3
- Qualifier
- possibly independent of clathrin-mediated endocytic protein traffickinge3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e2“high SA treatment severely affected auxin accumulation, root-specific PIN2 distribution and PIN2 gene transcription”
e3“promoted the vacuolar degradation of PIN2, possibly independent of clathrin-mediated endocytic protein trafficking”
Claim · model-written summary
SA-affected gravitropic root growth is explained by regulation of PIN2 gene transcription and PIN2 protein endocytosis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- PIN2e4
- Outcome
- gravitropic root growthe4
- Qualifier
- regulation of PIN2 gene transcription and PIN2 protein endocytosise4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e4“Our findings proposed a new underlying mechanism of SA-affected gravitropic root growth and root hair development via the regulation of PIN2 gene transcription and PIN2 protein endocytosis”
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