Abstract
Plant pathogenic fungi of the genus Fusarium cause agriculturally important diseases of small grain cereals and maize. Trichothecenes are a class of mycotoxins produced by different Fusarium species that inhibit eukaryotic protein biosynthesis and presumably interfere with the expression of genes induced during the defense response of the plants. One of its members, deoxynivalenol, most likely acts as a virulence factor during fungal pathogenesis and frequently accumulates in grain to levels posing a threat to human and animal health. We report the isolation and characterization of a gene from Arabidopsis thaliana encoding a UDP-glycosyltransferase that is able to detoxify deoxynivalenol. The enzyme, previously assigned the identifier UGT73C5, catalyzes the transfer of glucose from UDP-glucose to the hydroxyl group at carbon 3 of deoxynivalenol. Using a wheat germ extract-coupled transcription/translation system we have shown that this enzymatic reaction inactivates the mycotoxin. This deoxynivalenol-glucosyltransferase (DOGT1) was also found to detoxify the acetylated derivative 15-acetyl-deoxynivalenol, whereas no protective activity was observed against the structurally similar nivalenol. Expression of the glucosyltransferase is developmentally regulated and induced by deoxynivalenol as well as salicylic acid, ethylene, and jasmonic acid. Constitutive overexpression in Arabidopsis leads to enhanced tolerance against deoxynivalenol.
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Claims 11
Claim · model-written summary
An Arabidopsis thaliana gene encoding a deoxynivalenol-detoxifying UDP-glycosyltransferase was isolated.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- UDP-glycosyltransferasee0
- Outcome
- able to detoxify deoxynivalenole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolation and characterization of a gene from Arabidopsis thaliana encoding a UDP-glycosyltransferase that is able to detoxify deoxynivalenol.”
Claim · model-written summary
UGT73C5 transfers glucose from UDP-glucose to carbon 3 of deoxynivalenol.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- UGT73C5e0
- Outcome
- transfer of glucose from UDP-glucose to the hydroxyl group at carbon 3 of deoxynivalenole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme, previously assigned the identifier UGT73C5, catalyzes the transfer of glucose from UDP-glucose to the hydroxyl group at carbon 3 of deoxynivalenol.”
e1“gene from Arabidopsis thaliana”
Claim · model-written summary
The glucosyltransferase reaction inactivated the mycotoxin in a wheat germ extract system.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- inactivates the mycotoxine0
- Setting
- wheat germ extract-coupled transcription/translation systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using a wheat germ extract-coupled transcription/translation system we have shown that this enzymatic reaction inactivates the mycotoxin.”
e1“gene from Arabidopsis thaliana”
Claim · model-written summary
DOGT1 detoxified 15-acetyl-deoxynivalenol.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- DOGT1e0
- Outcome
- detoxify the acetylated derivative 15-acetyl-deoxynivalenole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This deoxynivalenol-glucosyltransferase (DOGT1) was also found to detoxify the acetylated derivative 15-acetyl-deoxynivalenol”
e1“gene from Arabidopsis thaliana”
Claim · model-written summary
DOGT1 showed no protective activity against nivalenol.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Gene
- DOGT1e1
- Outcome
- no protective activity was observed against the structurally similar nivalenole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no protective activity was observed against the structurally similar nivalenol.”
e1“deoxynivalenol-glucosyltransferase (DOGT1)”
e2“gene from Arabidopsis thaliana”
Claim · model-written summary
Glucosyltransferase expression was developmentally regulated.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- glucosyltransferasee0
- Outcome
- Expression of the glucosyltransferase is developmentally regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the glucosyltransferase is developmentally regulated”
e1“gene from Arabidopsis thaliana”
Claim · model-written summary
Deoxynivalenol induced glucosyltransferase expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- deoxynivalenole0
- Gene
- glucosyltransferasee0
- Outcome
- Expression of the glucosyltransferasee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the glucosyltransferase is developmentally regulated and induced by deoxynivalenol as well as salicylic acid, ethylene, and jasmonic acid.”
e1“gene from Arabidopsis thaliana”
Claim · model-written summary
Salicylic acid induced glucosyltransferase expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- salicylic acide0
- Gene
- glucosyltransferasee0
- Outcome
- Expression of the glucosyltransferasee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the glucosyltransferase is developmentally regulated and induced by deoxynivalenol as well as salicylic acid, ethylene, and jasmonic acid.”
e1“gene from Arabidopsis thaliana”
Claim · model-written summary
Ethylene induced glucosyltransferase expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- ethylenee0
- Gene
- glucosyltransferasee0
- Outcome
- Expression of the glucosyltransferasee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the glucosyltransferase is developmentally regulated and induced by deoxynivalenol as well as salicylic acid, ethylene, and jasmonic acid.”
e1“gene from Arabidopsis thaliana”
Claim · model-written summary
Jasmonic acid induced glucosyltransferase expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- jasmonic acide0
- Gene
- glucosyltransferasee0
- Outcome
- Expression of the glucosyltransferasee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the glucosyltransferase is developmentally regulated and induced by deoxynivalenol as well as salicylic acid, ethylene, and jasmonic acid.”
e1“gene from Arabidopsis thaliana”
Claim · model-written summary
Constitutive overexpression enhanced Arabidopsis tolerance to deoxynivalenol.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Constitutive overexpressione0
- Outcome
- tolerance against deoxynivalenole0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Constitutive overexpression in Arabidopsis leads to enhanced tolerance against deoxynivalenol.”
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See all 28 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.