Abstract
Plant hormones are chemical compounds that serve as crucial signal molecules. The growth and induction of bioactive compounds in plant suspension cultures depend on the exogenous application of auxins and cytokinins at different concentrations. In this study, date palm (Phoenix dactylifera L., cv. Shishi) cell suspension cultures initiated in Murashige and Skoog (MS) medium were treated with 2.5 and 5 mg/L 2-isopentenyladenine (2-iP) in combinations with 1, 2.5, 5 and 10 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), and compared to the standard cell multiplication medium as the control, which contained 1.5 mg/L 2iP and 10 mg/L naphthaleneacetic acid (NAA). The optimum biomass accumulation, based on the packed cell volume and dry weight, was found in the cell suspension culture augmented with 1 mg/L 2,4-D + 5 mg/L 2iP followed by 5 mg/L 2,4-D + 2.5 mg/L 2-iP. In addition, the study assessed the total phenolic, flavonoid and radical scavenging activity, and high performance liquid chromatography (HPLC) was also used to determine the content of polyphenols (apigenin, caffeic acid, catechin and kaempferol) quantitatively. Cell suspension cultures containing 5 mg/L 2,4-D and 2.5 mg/L 2iP yielded the maximum accumulation of phenolics, flavonoids and radical scavenging activity (90.65%), and also a significantly higher content of caffeic acid (37.1 µg/g DW). The present finding facilitates the scaling up and commercial production of polyphenols from date palm cell suspension culture.
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Claims 5
Claim · model-written summary
Date palm cultures with 1 mg/L 2,4-D plus 5 mg/L 2iP had optimum biomass accumulation.
- Taxon
- Date Palm
Phoenix dactyliferat0 - Treatment
- 1 mg/L 2,4-D + 5 mg/L 2iPe0
- Outcome
- biomass accumulatione0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimum biomass accumulation, based on the packed cell volume and dry weight, was found in the cell suspension culture augmented with 1 mg/L 2,4-D + 5 mg/L 2iP followed by 5 mg/L 2,4-D + 2.5 mg/L 2-iP”
t0“Date Palm (Phoenix dactylifera L.)”
Claim · model-written summary
The 5 mg/L 2,4-D plus 2.5 mg/L 2-iP treatment ranked second for biomass accumulation.
- Taxon
- Date PalmPhoenix dactyliferat0
- Treatment
- 5 mg/L 2,4-D + 2.5 mg/L 2-iPe0
- Outcome
- biomass accumulatione0
- Direction
- followed bye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“biomass accumulation, based on the packed cell volume and dry weight, was found in the cell suspension culture augmented with 1 mg/L 2,4-D + 5 mg/L 2iP followed by 5 mg/L 2,4-D + 2.5 mg/L 2-iP”
t0“Date Palm (Phoenix dactylifera L.)”
Claim · model-written summary
Cultures with 5 mg/L 2,4-D and 2.5 mg/L 2iP maximized phenolics and flavonoids.
- Taxon
- Date PalmPhoenix dactyliferat0
- Treatment
- 5 mg/L 2,4-D and 2.5 mg/L 2iPe0
- Outcome
- phenolics, flavonoidse0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cell suspension cultures containing 5 mg/L 2,4-D and 2.5 mg/L 2iP yielded the maximum accumulation of phenolics, flavonoids and radical scavenging activity (90.65%)”
t0“Date Palm (Phoenix dactylifera L.)”
Claim · model-written summary
The same culture treatment maximized radical scavenging activity at 90.65%.
- Taxon
- Date PalmPhoenix dactyliferat0
- Treatment
- 5 mg/L 2,4-D and 2.5 mg/L 2iPe0
- Outcome
- radical scavenging activitye0
- Direction
- maximume0
- Value
- 90.65%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cell suspension cultures containing 5 mg/L 2,4-D and 2.5 mg/L 2iP yielded the maximum accumulation of phenolics, flavonoids and radical scavenging activity (90.65%)”
t0“Date Palm (Phoenix dactylifera L.)”
Claim · model-written summary
The same culture treatment gave significantly higher caffeic acid content of 37.1 µg/g DW.
- Taxon
- Date PalmPhoenix dactyliferat0
- Treatment
- 5 mg/L 2,4-D and 2.5 mg/L 2iPe0
- Outcome
- content of caffeic acide0
- Direction
- highere0
- Value
- 37.1 µg/g DWe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cell suspension cultures containing 5 mg/L 2,4-D and 2.5 mg/L 2iP yielded the maximum accumulation of phenolics, flavonoids and radical scavenging activity (90.65%), and also a significantly higher content of caffeic acid (37.1 µg/g DW)”
t0“Date Palm (Phoenix dactylifera L.)”
The same questions in other papers
2,4-D · growth · Phoenix dactylifera (date palm)Positive and negative results
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- positive
- negative
- no effect
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See all 25 papers →2,4-D · molecular: metabolites · Phoenix dactylifera (date palm)Results agree
1 paper in the same direction as this paper
- positive
2,4-D · quality: phytochemicals · Phoenix dactylifera (date palm)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.