Abstract
Banana propagation is dependent on propagation by tissue culture for industrial purposes. Due to lack of native endophytes in tissue culture plantlets, reintroduction of beneficial microorganisms to tissue culture plantlets become popular as a useful foundation for improving the level of establishment, increasing plant growth parameters, protecting the plantlets against pest and diseases and overall performance for field plantation. In the present study, rhizospheric and endophytic four bacterial species (Pseudomonas fluorescens, Bacillus putida, Bacillus pumilus, Bacillus amyloliquefaciens were isolated from different parts of healthy banana plants such as root, leaf and pseudostem. Banana tissue culture plantlets were cultured for root formation stage on MS medium and used as host for artificial inoculation. Then, bacterial inoculation was carried out with tissue culture plantlets both in vitro and ex vitro conditions. All banana plantlets used for this experiment were successfully acclimatized and survived in the greenhouse. The experiment consisted of three treatments: (1) bacterial inoculum with in vitro rooted plantlets for 2 weeks (2) bacterial inoculum with ex vitro (hardening) plantlets (3) control with sterilized distilled water. Among these treatments, in vitro inoculation affected significantly increased plant height, number of leaves and plant girth for all data collection times with the highest increment indices: (24.0, 7.0, and 7.3). Ex vitro inoculation showed the second highest increment indices: (22.0, 6.9 and 6.3) for plant height, number of leaves and plant girth, respectively. The lowest number of plant growth parameters were observed in control treatment for all four data collection time (19.0, 6.2, and 6.0). According to the results, application of mixtures of bacterial inoculum was effective for enhanced plant growth parameters of tissue culture banana plantlets under greenhouse condition. In addition, two weeks artificial inoculation during in vitro plantlets stage was found the most suitable method for application of microbes with tissue culture banana plantlets which can promote the microbial rhizosphere of banana prior to field plantation.
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Claims 9
Claim · model-written summary
All experimental banana plantlets acclimatized and survived in the greenhouse.
Verbatim
e0“All banana plantlets used for this experiment were successfully acclimatized and survived in the greenhouse.”
Claim · model-written summary
In vitro inoculation significantly increased banana plant height, with index 24.0.
Not reportedComparator
Verbatim
e0“in vitro inoculation affected significantly increased plant height, number of leaves and plant girth for all data collection times with the highest increment indices: (24.0, 7.0, and 7.3).”
e1“Banana tissue culture plantlets”
Claim · model-written summary
In vitro inoculation significantly increased leaf number, with index 7.0.
Not reportedComparator
Verbatim
e0“in vitro inoculation affected significantly increased plant height, number of leaves and plant girth for all data collection times with the highest increment indices: (24.0, 7.0, and 7.3).”
e1“Banana tissue culture plantlets”
Claim · model-written summary
In vitro inoculation significantly increased plant girth, with index 7.3.
Not reportedComparator
Verbatim
e0“in vitro inoculation affected significantly increased plant height, number of leaves and plant girth for all data collection times with the highest increment indices: (24.0, 7.0, and 7.3).”
e1“Banana tissue culture plantlets”
Claim · model-written summary
Ex vitro inoculation gave the second highest plant height index, 22.0.
- Taxon
- banana plantletse1
- Treatment
- Ex vitro inoculatione0
- Outcome
- plant heighte0
- Direction
- second higheste0
- Value
- 22.0e0
Not reportedComparator
Verbatim
e0“Ex vitro inoculation showed the second highest increment indices: (22.0, 6.9 and 6.3) for plant height, number of leaves and plant girth, respectively.”
e1“All banana plantlets used for this experiment”
Claim · model-written summary
Ex vitro inoculation gave the second highest leaf number index, 6.9.
- Taxon
- banana plantletse1
- Treatment
- Ex vitro inoculatione0
- Outcome
- number of leavese0
- Direction
- second higheste0
- Value
- 6.9e0
Not reportedComparator
Verbatim
e0“Ex vitro inoculation showed the second highest increment indices: (22.0, 6.9 and 6.3) for plant height, number of leaves and plant girth, respectively.”
e1“All banana plantlets used for this experiment”
Claim · model-written summary
Ex vitro inoculation gave the second highest plant girth index, 6.3.
- Taxon
- banana plantletse1
- Treatment
- Ex vitro inoculatione0
- Outcome
- plant girthe0
- Direction
- second higheste0
- Value
- 6.3e0
Not reportedComparator
Verbatim
e0“Ex vitro inoculation showed the second highest increment indices: (22.0, 6.9 and 6.3) for plant height, number of leaves and plant girth, respectively.”
e1“All banana plantlets used for this experiment”
Claim · model-written summary
The control had the lowest plant growth parameters across collection times.
Verbatim
e0“The lowest number of plant growth parameters were observed in control treatment for all four data collection time (19.0, 6.2, and 6.0).”
e1“All banana plantlets used for this experiment”
Claim · model-written summary
Two weeks of artificial inoculation during the in vitro stage was judged the most suitable microbial application method.
Verbatim
e0“two weeks artificial inoculation during in vitro plantlets stage was found the most suitable method for application of microbes with tissue culture banana plantlets”
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