Abstract
Cytokinin is considered to be an important driver of seed yield. To increase the yield of cotton while avoiding the negative consequences caused by constitutive overproduction of cytokinin, we down-regulated specifically the carpel genes for cytokinin oxidase/dehydrogenase (CKX), a key negative regulator of cytokinin levels, in transgenic cotton. The carpel-specific down-regulation of CKXs significantly enhanced cytokinin levels in the carpels. The elevated cytokinin promoted the expression of carpel- and ovule-development-associated genes, GhSTK2, GhAG1, and GhSHP, boosting ovule formation and thus producing more seeds in the ovary. Field experiments showed that the carpel-specific increase of cytokinin significantly increased both seed yield and fiber yield of cotton, without resulting in detrimental phenotypes. Our study details the regulatory mechanism of cytokinin signaling for seed development, and provides an effective and feasible strategy for yield improvement of seed crops.
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Claims 6
Claim · model-written summary
Carpel CKX down-regulation significantly raised cytokinin levels.
- Taxon
- cotton
Gossypium hirsutumt0 - Organ / stage
- carpelse0
- Treatment
- carpel-specific down-regulation of CKXse0
- Gene
- CKXse0
- Outcome
- cytokinin levelse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The carpel-specific down-regulation of CKXs significantly enhanced cytokinin levels in the carpels.”
t0“cotton”
Claim · model-written summary
Elevated cytokinin promoted expression of three carpel and ovule development genes.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhSTK2, GhAG1, and GhSHPe0
- Compound
- cytokinine0
- Outcome
- expression of carpel- and ovule-development-associated genese0
- Direction
- promotede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The elevated cytokinin promoted the expression of carpel- and ovule-development-associated genes, GhSTK2, GhAG1, and GhSHP”
t0“cotton”
Claim · model-written summary
Elevated cytokinin boosted ovule formation and seed number.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- ovarye0
- Compound
- cytokinine1
- Outcome
- ovule formation and thus producing more seedse0
- Direction
- boostinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“boosting ovule formation and thus producing more seeds in the ovary.”
e1“The elevated cytokinin”
t0“cotton”
Claim · model-written summary
Carpel-specific cytokinin increase significantly increased seed yield in field experiments.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- carpel-specific increase of cytokinine0
- Outcome
- seed yielde0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Field experiments showed that the carpel-specific increase of cytokinin significantly increased both seed yield and fiber yield of cotton, without resulting in detrimental phenotypes.”
Claim · model-written summary
Carpel-specific cytokinin increase significantly increased fiber yield in field experiments.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- carpel-specific increase of cytokinine0
- Outcome
- fiber yielde0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Field experiments showed that the carpel-specific increase of cytokinin significantly increased both seed yield and fiber yield of cotton, without resulting in detrimental phenotypes.”
Claim · model-written summary
Carpel-specific cytokinin increase caused no detrimental phenotypes.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- carpel-specific increase of cytokinine0
- Outcome
- without resulting in detrimental phenotypese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Field experiments showed that the carpel-specific increase of cytokinin significantly increased both seed yield and fiber yield of cotton, without resulting in detrimental phenotypes.”
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