Abstract
As a multigenic trait, rice tillering can optimize plant architecture for the maximum agronomic yield. SQUAMOSA PROMOTER BINDING PROTEIN-LIKE14 (OsSPL14) has been demonstrated to be necessary and sufficient to inhibit rice branching, but the underlying mechanism remains largely unclear. Here, we demonstrated that OsSPL14, which is cleaved by miR529 and miR156, inhibits tillering by fine-tuning auxin transport in rice. RNA interference of OsSPL14 or miR529 and miR156 overexpression significantly increased the tiller number, whereas OsSPL14 overexpression decreased the tiller number. Histological analysis revealed that the OsSPL14-overexpressing line had normal initiation of axillary buds but inhibited outgrowth of tillers. Moreover, OsSPL14 was found to be responsive to indole-acetic acid and 1-naphthylphthalamic acid, and RNA interference of OsSPL14 reduced polar auxin transport and increased 1-naphthylphthalamic acid sensitivity of rice plants. Further analysis revealed that OsSPL14 directly binds to the promoter of PIN-FORMED 1b (OsPIN1b) and PIN-LIKE6b (PILS6b) to regulate their expression positively. OsPIN1b and PILS6b were highly expressed in axillary buds and proved involved in bud outgrowth. Loss of function of OsPIN1b or PILS6b increased the tiller number of rice. Taken together, our findings suggested that OsSPL14 could control axillary bud outgrowth and tiller number by activating the expression of OsPIN1b and PILS6b to fine-tune auxin transport in rice.
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Claims 12
Claim · model-written summary
OsSPL14 inhibited tillering through auxin transport.
- Taxon
- rice
Oryza sativae0 - Gene
- OsSPL14e0
- Outcome
- tilleringe0
- Direction
- inhibitse0
- Qualifier
- fine-tuning auxin transporte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsSPL14, which is cleaved by miR529 and miR156, inhibits tillering by fine-tuning auxin transport in rice.”
Claim · model-written summary
OsSPL14 RNA interference increased tiller number.
- Taxon
- riceOryza sativat0
- Treatment
- RNA interference of OsSPL14e0
- Gene
- OsSPL14e0
- Outcome
- tiller numbere0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA interference of OsSPL14 or miR529 and miR156 overexpression significantly increased the tiller number, whereas OsSPL14 overexpression decreased the tiller number.”
t0“in rice”
Claim · model-written summary
miR529 overexpression increased tiller number.
- Taxon
- riceOryza sativat0
- Treatment
- miR529e0
- Outcome
- tiller numbere0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA interference of OsSPL14 or miR529 and miR156 overexpression significantly increased the tiller number, whereas OsSPL14 overexpression decreased the tiller number.”
t0“in rice”
Claim · model-written summary
miR156 overexpression increased tiller number.
- Taxon
- riceOryza sativat0
- Treatment
- miR156 overexpressione0
- Outcome
- tiller numbere0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA interference of OsSPL14 or miR529 and miR156 overexpression significantly increased the tiller number, whereas OsSPL14 overexpression decreased the tiller number.”
t0“in rice”
Claim · model-written summary
OsSPL14 overexpression decreased tiller number.
- Taxon
- riceOryza sativat0
- Treatment
- OsSPL14 overexpressione0
- Outcome
- tiller numbere0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA interference of OsSPL14 or miR529 and miR156 overexpression significantly increased the tiller number, whereas OsSPL14 overexpression decreased the tiller number.”
t0“in rice”
Claim · model-written summary
OsSPL14 overexpression left axillary bud initiation normal but inhibited tiller outgrowth.
- Taxon
- riceOryza sativat0
- Material
- OsSPL14-overexpressing linee0
- Outcome
- initiation of axillary budse0
- Direction
- normale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the OsSPL14-overexpressing line had normal initiation of axillary buds but inhibited outgrowth of tillers.”
t0“in rice”
Claim · model-written summary
OsSPL14 RNA interference reduced polar auxin transport.
- Taxon
- rice plantsOryza sativae0
- Treatment
- RNA interference of OsSPL14e0
- Outcome
- polar auxin transporte0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA interference of OsSPL14 reduced polar auxin transport and increased 1-naphthylphthalamic acid sensitivity of rice plants.”
Claim · model-written summary
OsSPL14 RNA interference increased sensitivity to 1-naphthylphthalamic acid.
- Taxon
- rice plantsOryza sativae0
- Treatment
- RNA interference of OsSPL14e0
- Outcome
- 1-naphthylphthalamic acid sensitivitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA interference of OsSPL14 reduced polar auxin transport and increased 1-naphthylphthalamic acid sensitivity of rice plants.”
Claim · model-written summary
OsSPL14 binds promoters of OsPIN1b and PILS6b and positively regulates their expression.
- Taxon
- riceOryza sativat0
- Gene
- OsSPL14e0
- Outcome
- their expressione0
- Direction
- positivelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsSPL14 directly binds to the promoter of PIN-FORMED 1b (OsPIN1b) and PIN-LIKE6b (PILS6b) to regulate their expression positively.”
t0“in rice”
Claim · model-written summary
OsPIN1b and PILS6b were highly expressed in axillary buds.
- Taxon
- riceOryza sativat0
- Gene
- OsPIN1b and PILS6be0
- Outcome
- expressed in axillary budse0
- Direction
- highlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsPIN1b and PILS6b were highly expressed in axillary buds and proved involved in bud outgrowth.”
t0“in rice”
Claim · model-written summary
OsPIN1b loss of function increased rice tiller number.
- Taxon
- riceOryza sativae0
- Treatment
- Loss of function of OsPIN1be0
- Outcome
- tiller numbere0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss of function of OsPIN1b or PILS6b increased the tiller number of rice.”
Claim · model-written summary
PILS6b loss of function increased rice tiller number.
- Taxon
- riceOryza sativae0
- Treatment
- PILS6be0
- Outcome
- tiller numbere0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss of function of OsPIN1b or PILS6b increased the tiller number of rice.”
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