Paper · 2022 · Foods

Extraction and Evaluation of Bioactive Compounds from Date (Phoenix dactylifera) Seed Using Supercritical and Subcritical CO2 Techniques

Kashif Ghafoor; Md. Zaidul Islam Sarker; Fahad Y. Al-Juhaimi; et al.

9
claims
18
verbatim quotes
47
citations
2
research areas
✓
Open access · cc-by

Abstract

Date (Phoenix dactylifera) seed is a potential source of natural antioxidants, and the use of innovative green and low temperature antioxidant recovery techniques (using CO2 as solvent) such as supercritical fluid (SFE) and subcritical (SubCO2) extractions can improve their yields and quality in the extracts. SFE, SubCO2 and Soxhlet techniques were employed to enrich antioxidants in extracts from Sukari (SKSE), Ambara (AMSE), Majdool (MJSE) and Sagai (SGSE) date seeds. Extract yields were evaluated and modelled for SFE extract using response surface methodology. Significantly higher (p < 0.05) phenolics (143.48−274.98 mg GAE/100 g), flavonoids (78.35−141.78 mg QE/100 g), anthocyanins (0.39−1.00 mg/100 g), and carotenoid (1.42−1.91 mg BCE/100 g) contents were detected in extracts obtained using SFE and SubCO2 methods. The evaluation of in vitro antioxidant properties showed that SFE and SubCO2 seed extracts demonstrated promising antioxidant (13.42−23.83 µg AAE/mL), antiradical (228.76−109.69 µg/mL DPPH IC50), ferric reducing antioxidant power (1.43−2.10 mmol TE/100 g) and ABTS cation scavenging (375.74-717.45 µmol TE/100 g) properties that were significantly higher than Soxhlet extracts. Both SFE and SubCO2 techniques can be effectively utilized as innovative and environmentally friendly alternatives to obtain high quality antioxidant rich extracts from date seed. These extracts may have potential functional and nutraceutical applications.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 9

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

SFE and SubCO2 extracts had significantly higher phenolics content.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Organ / stage
Seedt0
Treatment
SFE and SubCO2 methodse2
Outcome
phenolicse2
Direction
highere2
Value
143.48−274.98 mg GAE/100 ge2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date (Phoenix dactylifera) Seed”

✓ located verbatim in the source · title, characters 54–85 · show in text ↑
e2

“Significantly higher (p < 0.05) phenolics (143.48−274.98 mg GAE/100 g), flavonoids (78.35−141.78 mg QE/100 g), anthocyanins (0.39−1.00 mg/100 g), and carotenoid (1.42−1.91 mg BCE/100 g) contents were detected in extracts obtained using SFE and SubCO2 methods”

✓ located verbatim in the source · abstract, characters 569–827 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

SFE and SubCO2 extracts had significantly higher flavonoids content.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Organ / stage
Seedt0
Treatment
SFE and SubCO2 methodse2
Outcome
flavonoidse2
Direction
highere2
Value
78.35−141.78 mg QE/100 ge2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date (Phoenix dactylifera) Seed”

✓ located verbatim in the source · title, characters 54–85 · show in text ↑
e2

“Significantly higher (p < 0.05) phenolics (143.48−274.98 mg GAE/100 g), flavonoids (78.35−141.78 mg QE/100 g), anthocyanins (0.39−1.00 mg/100 g), and carotenoid (1.42−1.91 mg BCE/100 g) contents were detected in extracts obtained using SFE and SubCO2 methods”

✓ located verbatim in the source · abstract, characters 569–827 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

SFE and SubCO2 extracts had significantly higher anthocyanins content.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Organ / stage
Seedt0
Treatment
SFE and SubCO2 methodse2
Outcome
anthocyaninse2
Direction
highere2
Value
0.39−1.00 mg/100 ge2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date (Phoenix dactylifera) Seed”

✓ located verbatim in the source · title, characters 54–85 · show in text ↑
e2

“Significantly higher (p < 0.05) phenolics (143.48−274.98 mg GAE/100 g), flavonoids (78.35−141.78 mg QE/100 g), anthocyanins (0.39−1.00 mg/100 g), and carotenoid (1.42−1.91 mg BCE/100 g) contents were detected in extracts obtained using SFE and SubCO2 methods”

✓ located verbatim in the source · abstract, characters 569–827 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

SFE and SubCO2 extracts had significantly higher carotenoid content.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Organ / stage
Seedt0
Treatment
SFE and SubCO2 methodse2
Outcome
carotenoide2
Direction
highere2
Value
1.42−1.91 mg BCE/100 ge2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date (Phoenix dactylifera) Seed”

✓ located verbatim in the source · title, characters 54–85 · show in text ↑
e2

“Significantly higher (p < 0.05) phenolics (143.48−274.98 mg GAE/100 g), flavonoids (78.35−141.78 mg QE/100 g), anthocyanins (0.39−1.00 mg/100 g), and carotenoid (1.42−1.91 mg BCE/100 g) contents were detected in extracts obtained using SFE and SubCO2 methods”

✓ located verbatim in the source · abstract, characters 569–827 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

SFE and SubCO2 seed extracts had significantly higher antioxidant activity than Soxhlet extracts.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Organ / stage
Seedt0
Treatment
SFE and SubCO2 seed extractse3
Outcome
antioxidante3
Direction
highere3
Value
13.42−23.83 µg AAE/mLe3
Comparator
Soxhlet extractse3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date (Phoenix dactylifera) Seed”

✓ located verbatim in the source · title, characters 54–85 · show in text ↑
e3

“The evaluation of in vitro antioxidant properties showed that SFE and SubCO2 seed extracts demonstrated promising antioxidant (13.42−23.83 µg AAE/mL), antiradical (228.76−109.69 µg/mL DPPH IC50), ferric reducing antioxidant power (1.43−2.10 mmol TE/100 g) and ABTS cation scavenging (375.74-717.45 µmol TE/100 g) properties that were significantly higher than Soxhlet extracts”

✓ located verbatim in the source · abstract, characters 829–1205 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

SFE and SubCO2 seed extracts had significantly higher antiradical activity (DPPH IC50) than Soxhlet extracts, as written.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Organ / stage
Seedt0
Treatment
SFE and SubCO2 seed extractse3
Outcome
antiradicale3
Direction
highere3
Value
228.76−109.69 µg/mL DPPH IC50e3
Comparator
Soxhlet extractse3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date (Phoenix dactylifera) Seed”

✓ located verbatim in the source · title, characters 54–85 · show in text ↑
e3

“The evaluation of in vitro antioxidant properties showed that SFE and SubCO2 seed extracts demonstrated promising antioxidant (13.42−23.83 µg AAE/mL), antiradical (228.76−109.69 µg/mL DPPH IC50), ferric reducing antioxidant power (1.43−2.10 mmol TE/100 g) and ABTS cation scavenging (375.74-717.45 µmol TE/100 g) properties that were significantly higher than Soxhlet extracts”

✓ located verbatim in the source · abstract, characters 829–1205 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

SFE and SubCO2 seed extracts had significantly higher ferric reducing antioxidant power than Soxhlet extracts.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Organ / stage
Seedt0
Treatment
SFE and SubCO2 seed extractse3
Outcome
ferric reducing antioxidant powere3
Direction
highere3
Value
1.43−2.10 mmol TE/100 ge3
Comparator
Soxhlet extractse3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date (Phoenix dactylifera) Seed”

✓ located verbatim in the source · title, characters 54–85 · show in text ↑
e3

“The evaluation of in vitro antioxidant properties showed that SFE and SubCO2 seed extracts demonstrated promising antioxidant (13.42−23.83 µg AAE/mL), antiradical (228.76−109.69 µg/mL DPPH IC50), ferric reducing antioxidant power (1.43−2.10 mmol TE/100 g) and ABTS cation scavenging (375.74-717.45 µmol TE/100 g) properties that were significantly higher than Soxhlet extracts”

✓ located verbatim in the source · abstract, characters 829–1205 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

SFE and SubCO2 seed extracts had significantly higher ABTS cation scavenging than Soxhlet extracts.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Organ / stage
Seedt0
Treatment
SFE and SubCO2 seed extractse3
Outcome
ABTS cation scavenginge3
Direction
highere3
Value
375.74-717.45 µmol TE/100 ge3
Comparator
Soxhlet extractse3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date (Phoenix dactylifera) Seed”

✓ located verbatim in the source · title, characters 54–85 · show in text ↑
e3

“The evaluation of in vitro antioxidant properties showed that SFE and SubCO2 seed extracts demonstrated promising antioxidant (13.42−23.83 µg AAE/mL), antiradical (228.76−109.69 µg/mL DPPH IC50), ferric reducing antioxidant power (1.43−2.10 mmol TE/100 g) and ABTS cation scavenging (375.74-717.45 µmol TE/100 g) properties that were significantly higher than Soxhlet extracts”

✓ located verbatim in the source · abstract, characters 829–1205 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

SFE and SubCO2 can be used as alternatives to obtain high quality antioxidant rich extracts from date seed.

Taxon
Phoenix dactyliferaPhoenix dactyliferat0
Outcome
high quality antioxidant rich extractse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date (Phoenix dactylifera) Seed”

✓ located verbatim in the source · title, characters 54–85 · show in text ↑
e4

“Both SFE and SubCO2 techniques can be effectively utilized as innovative and environmentally friendly alternatives to obtain high quality antioxidant rich extracts from date seed”

✓ located verbatim in the source · abstract, characters 1207–1385 · show in text ↑

botanical · quality: phytochemicals · Phoenix dactylifera (date palm)Positive and negative results

11 papers: 10 positive, 1 negative or no effect

9 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. positive

7 papers more in this group

See all 11 papers →

botanical · molecular: metabolites · Phoenix dactylifera (date palm)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.