Abstract
Phytohormone abscisic acid (ABA) and plant-specific WRKY transcription factors (TFs) have been implicated to play important roles in various stress responses. The involvement of WRKY TFs in ABA-mediated cold tolerance of economical fruits, such as banana fruit, however remains largely unknown. Here, we reported that ABA application could induce expressions of ABA biosynthesis-related genes MaNCED1 and MaNCED2, increase endogenous ABA contents, and thereby enhance cold tolerance in banana fruit. Four banana fruit WRKY TFs, designated as MaWRKY31, MaWRKY33, MaWRKY60, and MaWRKY71, were identified and characterized. All four of these MaWRKYs were nuclear-localized and displayed transactivation activities. Their expressions were induced by ABA treatment during cold storage. More importantly, the gel mobility shift assay and transient expression analysis revealed that MaWRKY31, MaWRKY33, MaWRKY60, and MaWRKY71 directly bound to the W-box elements in MaNCED1 and MaNCED2 promoters and activated their expressions. Taken together, our findings demonstrate that banana fruit WRKY TFs are involved in ABA-induced cold tolerance by, at least in part, increasing ABA levels via directly activating NECD expressions.
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Claims 9
Claim · model-written summary
ABA application could induce expression of ABA biosynthesis-related genes MaNCED1 and MaNCED2 in banana fruit.
- Taxon
- banana fruite1
- Treatment
- ABA applicatione1
- Gene
- MaNCED1 and MaNCED2e1
- Outcome
- expressions of ABA biosynthesis-related genese1
- Direction
- inducee1
Not reportedComparator
Verbatim
e1“ABA application could induce expressions of ABA biosynthesis-related genes MaNCED1 and MaNCED2, increase endogenous ABA contents, and thereby enhance cold tolerance in banana fruit”
Claim · model-written summary
ABA application increased endogenous ABA contents in banana fruit.
Not reportedComparator
Verbatim
e1“ABA application could induce expressions of ABA biosynthesis-related genes MaNCED1 and MaNCED2, increase endogenous ABA contents, and thereby enhance cold tolerance in banana fruit”
Claim · model-written summary
ABA application enhanced cold tolerance in banana fruit.
Not reportedComparator
Verbatim
e1“ABA application could induce expressions of ABA biosynthesis-related genes MaNCED1 and MaNCED2, increase endogenous ABA contents, and thereby enhance cold tolerance in banana fruit”
Claim · model-written summary
Four banana fruit WRKY transcription factors, MaWRKY31, MaWRKY33, MaWRKY60 and MaWRKY71, were identified and characterized.
Verbatim
e2“Four banana fruit WRKY TFs, designated as MaWRKY31, MaWRKY33, MaWRKY60, and MaWRKY71, were identified and characterized”
Claim · model-written summary
All four MaWRKYs were nuclear-localized and displayed transactivation activities in banana fruit.
Verbatim
e2“Four banana fruit WRKY TFs, designated as MaWRKY31, MaWRKY33, MaWRKY60, and MaWRKY71, were identified and characterized”
e3“All four of these MaWRKYs were nuclear-localized and displayed transactivation activities”
Claim · model-written summary
ABA treatment during cold storage induced expression of the four banana fruit MaWRKYs.
- Taxon
- banana fruite2
- Treatment
- ABA treatmente4
- Gene
- MaWRKY31, MaWRKY33, MaWRKY60, and MaWRKY71e2
- Outcome
- expressionse4
- Direction
- inducede4
- Setting
- cold storagee4
Not reportedComparator
Verbatim
e2“Four banana fruit WRKY TFs, designated as MaWRKY31, MaWRKY33, MaWRKY60, and MaWRKY71, were identified and characterized”
e4“Their expressions were induced by ABA treatment during cold storage”
Claim · model-written summary
MaWRKY31, MaWRKY33, MaWRKY60 and MaWRKY71 directly bound to the W-box elements in MaNCED1 and MaNCED2 promoters.
Verbatim
e2“Four banana fruit WRKY TFs, designated as MaWRKY31, MaWRKY33, MaWRKY60, and MaWRKY71, were identified and characterized”
e5“the gel mobility shift assay and transient expression analysis revealed that MaWRKY31, MaWRKY33, MaWRKY60, and MaWRKY71 directly bound to the W-box elements in MaNCED1 and MaNCED2 promoters”
Claim · model-written summary
MaWRKY31, MaWRKY33, MaWRKY60 and MaWRKY71 activated the expression of MaNCED1 and MaNCED2 in banana fruit.
Verbatim
e2“Four banana fruit WRKY TFs, designated as MaWRKY31, MaWRKY33, MaWRKY60, and MaWRKY71, were identified and characterized”
e5“the gel mobility shift assay and transient expression analysis revealed that MaWRKY31, MaWRKY33, MaWRKY60, and MaWRKY71 directly bound to the W-box elements in MaNCED1 and MaNCED2 promoters and activated their expressions”
Claim · model-written summary
Banana fruit WRKY TFs are involved in ABA-induced cold tolerance, at least in part by increasing ABA levels through direct activation of NCED expression.
Verbatim
e6“banana fruit WRKY TFs are involved in ABA-induced cold tolerance by, at least in part, increasing ABA levels via directly activating NECD expressions”
The same questions in other papers
(+)-abscisic acid · molecular: metabolites · MusaPositive and negative results
1 in the opposite direction
- no effect
(+)-abscisic acid · molecular: expression · MusaResults agree
1 paper in the same direction as this paper
- positive
(+)-abscisic acid · tolerance: abiotic · MusaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.