Abstract
Low temperature stress poses a significant challenge to the productivity of horticultural crops. The dynamic expression of cold-responsive genes plays a crucial role in plant cold tolerance. While NAC transcription factors have been extensively studied in plant growth and development, their involvement in regulating plant cold tolerance remains poorly understood. In this study, we focused on the identification and characterisation of SlNAC3 as the most rapid and robust responsive gene in tomato under low temperature conditions. Manipulating SlNAC3 through overexpression or silencing resulted in reduced or enhanced cold tolerance, respectively. Surprisingly, we discovered a negative correlation between the expression of CBF and cold tolerance in the SlNAC3 transgenic lines. These findings suggest that SlNAC3 regulates tomato cold tolerance likely through a CBF-independent pathway. Furthermore, we conducted additional investigations to identify the molecular mechanisms underlying SINAC3-mediated cold tolerance in tomatoes. Our results revealed that SlNAC3 controls the transcription of ethylene biosynthetic genes, thereby bursting ethylene release in response to cold stress. Indeed, the silencing of these genes led to an augmentation in cold tolerance. This discovery provides valuable insights into the regulatory pathways involved in ethylene-mediated cold tolerance in tomatoes, offering potential strategies for developing innovative approaches to enhance cold stress resilience in this economically important crop species.
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Claims 6
Claim · model-written summary
SlNAC3 was the most rapid and robust low-temperature responsive gene in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- low temperature conditionse0
- Gene
- SlNAC3e0
- Outcome
- most rapid and robust responsive genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlNAC3 as the most rapid and robust responsive gene in tomato under low temperature conditions”
Claim · model-written summary
SlNAC3 overexpression reduced tomato cold tolerance.
- Taxon
- tomatoesSolanum lycopersicumt0
- Treatment
- overexpressione0
- Gene
- SlNAC3e0
- Outcome
- cold tolerancee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Manipulating SlNAC3 through overexpression or silencing resulted in reduced or enhanced cold tolerance, respectively”
t0“tomatoes”
Claim · model-written summary
SlNAC3 silencing enhanced tomato cold tolerance.
- Taxon
- tomatoesSolanum lycopersicumt0
- Treatment
- silencinge0
- Gene
- SlNAC3e0
- Outcome
- cold tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Manipulating SlNAC3 through overexpression or silencing resulted in reduced or enhanced cold tolerance, respectively”
t0“tomatoes”
Claim · model-written summary
CBF expression was negatively correlated with cold tolerance in SlNAC3 transgenic lines.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- SlNAC3 transgenic linese0
- Gene
- CBFe0
- Outcome
- cold tolerancee0
- Direction
- negative correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“negative correlation between the expression of CBF and cold tolerance in the SlNAC3 transgenic lines”
t0“tomatoes”
Claim · model-written summary
SlNAC3 controlled transcription of ethylene biosynthetic genes and increased ethylene release during cold stress.
- Taxon
- tomatoesSolanum lycopersicumt0
- Gene
- SlNAC3e0
- Outcome
- ethylene releasee0
- Qualifier
- in response to cold stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlNAC3 controls the transcription of ethylene biosynthetic genes, thereby bursting ethylene release in response to cold stress”
t0“tomatoes”
Claim · model-written summary
Silencing ethylene biosynthetic genes increased tomato cold tolerance.
- Taxon
- tomatoesSolanum lycopersicumt0
- Treatment
- silencing of these genese0
- Outcome
- cold tolerancee0
- Direction
- augmentatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the silencing of these genes led to an augmentation in cold tolerance”
t0“tomatoes”
The same questions in other papers
loss of function · tolerance: abiotic · Solanum lycopersicum (tomato)Positive and negative results
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