Paper · 2016 · Journal of Biological Control

Efficacy of Substrate Based Bioformulation of Microbial Antagonists in the Management of Bacterial Disease of some Solanaceous Vegetables in Assam

Popy Bora; L. C. Bora; P. C. Deka

12
claims
17
verbatim quotes
5
citations
2
research areas
✓
Open access

Abstract

A study was undertaken to explore effective organic substrate-based bioformulation using virulent cells of antagonists Pseudomonas fluorescens, Bacillus subtilis and Trichoderma viride during 2010-13. Three organic substrates, viz. vermicompost, farm yard manure and mustard oil cake (MOC) were compared for mass multiplication of the antagonists. All the substrate based antagonists showed effective results in suppression of bacterial wilt (Ralstonia solanacearum Yabuchi et al.) incidence in vegetable crops tomato, brinjal and chill. Quantitative aspect of population dynamics of the antagonists at different days of storage was made to evaluate the shelflife of the biopesticide and found that the antagonists maintained a steady population count upto 180 days of storage at room temperature. The combination of vermicompost P. fluorescens, carboxy methyl cellulose (CMC) and mannitol showed best shelf-life as it maintained highest population recovery of P. fluorescens and B. subtillis at different days of storage. The combination of MOC, T. viride, CMC and mannitol showed best shelf-life in case of T. viride and maintained highest population recovery of the antagonist at different days of storage. Application of the substrate based bioformulations as combination of seed treatment, root application, soil application at transplanting and soil application at 30 days after transplanting showed minimum wilt incidence and maximum yield in tomato, brinjal and chilli. Maximum disease reduction (81.85%) was shown by bioformulation comprising P. fluorescens with vermicompost as substrate followed by T. viride with MOC as substrate (79.07%). Following the trend of reduction in disease incidence, yield was maximum in tomato (36.0 t/ ha), when the crop was treated with vermicompost based P. fluorescens followed by treatment with MOC based T. viride (33.35 t/ha). Yield of brinjal (27.60 t/ha) and chilli (26.30 t/ha) was similarly maximum when bioformulation of vermicompost based P. fluorescens was applied.

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Claims 12

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Substrate-based microbial antagonists suppressed bacterial wilt incidence in tomato, brinjal, and chilli.

Taxon
tomato, brinjal and chille0
Treatment
substrate based antagonistse0
Interactor
Ralstonia solanacearum Yabuchi et al.Ralstonia solanacearume0pathogen
Outcome
bacterial wilt (Ralstonia solanacearum Yabuchi et al.) incidencee0
Direction
suppressione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ralstonia solanacearum
Ralstonia solanacearum · InteractorClemson University - USDA Cooperative Extension Slide Series, Bugwood.org · Public domain · Wikimedia Commons ↗

Verbatim

e0

“All the substrate based antagonists showed effective results in suppression of bacterial wilt (Ralstonia solanacearum Yabuchi et al.) incidence in vegetable crops tomato, brinjal and chill.”

✓ located verbatim in the source · abstract, characters 348–537 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Antagonist populations remained steady up to 180 days at room temperature.

Taxon
Solanaceous Vegetablest0
Outcome
antagonists maintained a steady population counte0
Value
180 dayse0
Setting
room temperaturee0

Verbatim

e0

“the antagonists maintained a steady population count upto 180 days of storage at room temperature.”

✓ located verbatim in the source · abstract, characters 699–797 · show in text ↑
t0

“Solanaceous Vegetables”

✓ located verbatim in the source · title, characters 115–137 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Vermicompost with P. fluorescens, CMC, and mannitol had the best shelf life and highest P. fluorescens and B. subtilis recovery.

Taxon
Solanaceous Vegetablest0
Treatment
vermicompost P. fluorescens, carboxy methyl cellulose (CMC) and mannitole0
Interactor
P. fluorescensPseudomonas fluorescense0biocontrol_agent
Outcome
best shelf-lifee0
Direction
beste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The combination of vermicompost P. fluorescens, carboxy methyl cellulose (CMC) and mannitol showed best shelf-life as it maintained highest population recovery of P. fluorescens and B. subtillis at different days of storage.”

✓ located verbatim in the source · abstract, characters 798–1022 · show in text ↑
t0

“Solanaceous Vegetables”

✓ located verbatim in the source · title, characters 115–137 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

MOC with T. viride, CMC, and mannitol gave the best T. viride shelf life and recovery.

Taxon
Solanaceous Vegetablest0
Treatment
MOC, T. viride, CMC and mannitole0
Interactor
T. virideTrichoderma viridee0biocontrol_agent
Outcome
best shelf-lifee0
Direction
beste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma viride
Trichoderma viride · InteractorKatja Schulz · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The combination of MOC, T. viride, CMC and mannitol showed best shelf-life in case of T. viride and maintained highest population recovery of the antagonist at different days of storage.”

✓ located verbatim in the source · abstract, characters 1023–1209 · show in text ↑
t0

“Solanaceous Vegetables”

✓ located verbatim in the source · title, characters 115–137 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combined seed, root, and soil application gave minimum wilt incidence.

Taxon
tomato, brinjal and chillie0
Treatment
combination of seed treatment, root application, soil application at transplanting and soil application at 30 days after transplantinge0
Outcome
wilt incidencee0
Direction
minimume0

Not reportedComparator

Verbatim

e0

“Application of the substrate based bioformulations as combination of seed treatment, root application, soil application at transplanting and soil application at 30 days after transplanting showed minimum wilt incidence and maximum yield in tomato, brinjal and chilli.”

✓ located verbatim in the source · abstract, characters 1210–1477 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combined seed, root, and soil application gave maximum vegetable yield.

Taxon
tomato, brinjal and chillie0
Treatment
combination of seed treatment, root application, soil application at transplanting and soil application at 30 days after transplantinge0
Outcome
yielde0
Direction
maximume0

Not reportedComparator

Verbatim

e0

“Application of the substrate based bioformulations as combination of seed treatment, root application, soil application at transplanting and soil application at 30 days after transplanting showed minimum wilt incidence and maximum yield in tomato, brinjal and chilli.”

✓ located verbatim in the source · abstract, characters 1210–1477 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Vermicompost-based P. fluorescens achieved 81.85% disease reduction.

Taxon
Solanaceous Vegetablest0
Treatment
P. fluorescens with vermicompost as substratee0
Interactor
P. fluorescensPseudomonas fluorescense0biocontrol_agent
Outcome
disease reductione0
Direction
Maximume0
Value
81.85%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Maximum disease reduction (81.85%) was shown by bioformulation comprising P. fluorescens with vermicompost as substrate followed by T. viride with MOC as substrate (79.07%).”

✓ located verbatim in the source · abstract, characters 1478–1651 · show in text ↑
t0

“Solanaceous Vegetables”

✓ located verbatim in the source · title, characters 115–137 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MOC-based T. viride achieved 79.07% disease reduction.

Taxon
Solanaceous Vegetablest0
Treatment
T. viride with MOC as substratee0
Interactor
T. virideTrichoderma viridee0biocontrol_agent
Outcome
disease reductione0
Value
79.07%e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma viride
Trichoderma viride · InteractorKatja Schulz · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Maximum disease reduction (81.85%) was shown by bioformulation comprising P. fluorescens with vermicompost as substrate followed by T. viride with MOC as substrate (79.07%).”

✓ located verbatim in the source · abstract, characters 1478–1651 · show in text ↑
t0

“Solanaceous Vegetables”

✓ located verbatim in the source · title, characters 115–137 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Tomato yield reached 36.0 t/ ha with vermicompost-based P. fluorescens.

Treatment
vermicompost based P. fluorescense0
Interactor
P. fluorescensPseudomonas fluorescense0biocontrol_agent
Outcome
yielde0
Direction
maximume0
Value
36.0 t/ hae0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“yield was maximum in tomato (36.0 t/ ha), when the crop was treated with vermicompost based P. fluorescens followed by treatment with MOC based T. viride (33.35 t/ha).”

✓ located verbatim in the source · abstract, characters 1707–1874 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Tomato yield was 33.35 t/ha with MOC-based T. viride.

Treatment
MOC based T. viridee0
Interactor
T. virideTrichoderma viridee0biocontrol_agent
Outcome
yielde0
Value
33.35 t/hae0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Trichoderma viride
Trichoderma viride · InteractorKatja Schulz · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“yield was maximum in tomato (36.0 t/ ha), when the crop was treated with vermicompost based P. fluorescens followed by treatment with MOC based T. viride (33.35 t/ha).”

✓ located verbatim in the source · abstract, characters 1707–1874 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Brinjal yield was highest at 27.60 t/ha with vermicompost-based P. fluorescens.

Treatment
vermicompost based P. fluorescense0
Interactor
P. fluorescensPseudomonas fluorescense0biocontrol_agent
Outcome
Yielde0
Direction
maximume0
Value
27.60 t/hae0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Yield of brinjal (27.60 t/ha) and chilli (26.30 t/ha) was similarly maximum when bioformulation of vermicompost based P. fluorescens was applied.”

✓ located verbatim in the source · abstract, characters 1875–2020 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chilli yield was highest at 26.30 t/ha with vermicompost-based P. fluorescens.

Taxon
chillie0
Treatment
vermicompost based P. fluorescense0
Interactor
P. fluorescensPseudomonas fluorescense0biocontrol_agent
Outcome
Yielde0
Direction
maximume0
Value
26.30 t/hae0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pseudomonas fluorescens
Pseudomonas fluorescens · InteractorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Yield of brinjal (27.60 t/ha) and chilli (26.30 t/ha) was similarly maximum when bioformulation of vermicompost based P. fluorescens was applied.”

✓ located verbatim in the source · abstract, characters 1875–2020 · show in text ↑

Pseudomonas fluorescens · yield · Solanum melongena (eggplant)Results agree

7 papers: 7 positive, 0 negative or no effect

6 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

3 papers more in this group

See all 7 papers →

Pseudomonas fluorescens · yield · Capsicum (peppers)Results agree

10 papers: 10 positive, 0 negative or no effect

9 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

Pseudomonas fluorescens · yield · Solanum lycopersicum (tomato)Results agree

12 papers: 12 positive, 0 negative or no effect

11 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

8 papers more in this group

See all 12 papers →

Trichoderma viride · yield · Solanum lycopersicum (tomato)Results agree

12 papers: 12 positive, 0 negative or no effect

11 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

8 papers more in this group

See all 12 papers →

fertilization · yield · Capsicum (peppers)Results differ

163 papers: 152 positive, 11 negative or no effect

151 papers in the same direction as this paper · 9 in the opposite direction · 2 reporting no difference from an alternative

  1. negative
  2. no effect
  3. no effect

159 papers more in this group

See all 163 papers →

transplanting · disease · Solanum melongena (eggplant)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

seed treatment · yield · Capsicum (peppers)Positive and negative results

15 papers: 14 positive, 1 negative or no effect

13 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

11 papers more in this group

See all 15 papers →

transplanting · yield · Solanum melongena (eggplant)Positive and negative results

16 papers: 14 positive, 2 negative or no effect

13 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

12 papers more in this group

See all 16 papers →

8 of 26 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.