Abstract
Soybean mosaic virus (SMV) is a member of the genus Potyvirus that extensively impairs global soybean production. The full-length coding sequence of the MADS-box transcription factor GmCAL was cloned from the SMV-resistant soybean cultivar Kefeng 1. SMV-induced expression analysis indicated that GmCAL responded quickly to SMV-SC8 infection in Kefeng 1 but not in NN1138-2. GmCAL was expressed at high levels in flowers and pods but at lower levels in leaves. The gene was localized to the nucleus by subcellular localization assay. Virus-induced gene silencing did not increase the accumulation of SMV in GmCAL-silenced Kefeng 1 plants (with silencing efficiency ∼ 80%) after SC8 inoculation. GmCAL-silencing plants still conferred resistance to SC8 that might be owing to incomplete silencing of genes with lower expression. SMV content decreased significantly in GmCAL-overexpressing NN1138-2 plants after SMV-SC3, SMV-SC7, and SMV-SC8 inoculation in comparison with a vector control, showing that overexpression of GmCAL conferred broad-spectrum resistance to multiple SMV strains. These results confirm that GmCAL, a key regulator but not a specific SC8 resistance gene (Rsc8), is a positive regulatory transcription factor involved in soybean resistance to SMV.
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Claims 8
Claim · model-written summary
GmCAL responded quickly to SMV-SC8 infection in soybean cultivar Kefeng 1, compared with NN1138-2.
- Taxon
- soybean
Glycine maxe0 - Material
- Kefeng 1e0
- Treatment
- SMV-SC8 infectione1
- Interactor
- SMV-SC8e1pathogen
- Gene
- GmCALe1
- Outcome
- SMV-induced expressione1
- Direction
- responded quicklye1
- Comparator
- NN1138-2e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The full-length coding sequence of the MADS-box transcription factor GmCAL was cloned from the SMV-resistant soybean cultivar Kefeng 1”
e1“SMV-induced expression analysis indicated that GmCAL responded quickly to SMV-SC8 infection in Kefeng 1 but not in NN1138-2”
Claim · model-written summary
GmCAL did not respond to SMV-SC8 infection in soybean cultivar NN1138-2.
- Taxon
- soybeanGlycine maxe0
- Material
- NN1138-2e1
- Treatment
- SMV-SC8 infectione1
- Interactor
- SMV-SC8e1pathogen
- Gene
- GmCALe1
- Outcome
- SMV-induced expressione1
- Direction
- no_changeinferrede1
- Comparator
- Kefeng 1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The full-length coding sequence of the MADS-box transcription factor GmCAL was cloned from the SMV-resistant soybean cultivar Kefeng 1”
e1“SMV-induced expression analysis indicated that GmCAL responded quickly to SMV-SC8 infection in Kefeng 1 but not in NN1138-2”
Claim · model-written summary
GmCAL was expressed at high levels in soybean flowers and pods but at lower levels in leaves.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- flowers and podse2
- Gene
- GmCALe2
- Outcome
- expressede2
- Qualifier
- at high levels in flowers and pods but at lower levels in leavese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The full-length coding sequence of the MADS-box transcription factor GmCAL was cloned from the SMV-resistant soybean cultivar Kefeng 1”
e2“GmCAL was expressed at high levels in flowers and pods but at lower levels in leaves”
Claim · model-written summary
GmCAL was localized to the nucleus.
- Taxon
- soybeanGlycine maxe0
- Gene
- GmCALe0
- Outcome
- subcellular localizatione3
- Qualifier
- to the nucleuse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The full-length coding sequence of the MADS-box transcription factor GmCAL was cloned from the SMV-resistant soybean cultivar Kefeng 1”
e3“The gene was localized to the nucleus by subcellular localization assay”
Claim · model-written summary
Virus-induced silencing of GmCAL did not increase SMV accumulation in Kefeng 1 plants.
- Taxon
- soybeanGlycine maxe0
- Material
- Kefeng 1e4
- Treatment
- Virus-induced gene silencinge4
- Interactor
- SMVe4pathogen
- Gene
- GmCALe4
- Outcome
- accumulation of SMVe4
- Direction
- did not increasee4
- Qualifier
- silencing efficiency ∼ 80%e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The full-length coding sequence of the MADS-box transcription factor GmCAL was cloned from the SMV-resistant soybean cultivar Kefeng 1”
e4“Virus-induced gene silencing did not increase the accumulation of SMV in GmCAL-silenced Kefeng 1 plants (with silencing efficiency ∼ 80%) after SC8 inoculation”
Claim · model-written summary
GmCAL-silenced plants still conferred resistance to SC8.
- Taxon
- soybeanGlycine maxe0
- Interactor
- SC8e5pathogen
- Gene
- GmCALe5
- Outcome
- resistance to SC8e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The full-length coding sequence of the MADS-box transcription factor GmCAL was cloned from the SMV-resistant soybean cultivar Kefeng 1”
e5“GmCAL-silencing plants still conferred resistance to SC8”
Claim · model-written summary
GmCAL-overexpressing NN1138-2 plants had significantly lower SMV content than vector controls after SMV-SC3, SMV-SC7 and SMV-SC8 inoculation.
- Taxon
- soybeanGlycine maxe0
- Material
- NN1138-2e6
- Treatment
- GmCAL-overexpressinge6
- Interactor
- SMVe6pathogen
- Gene
- GmCALe6
- Outcome
- SMV contente6
- Direction
- decreasede6
- Qualifier
- after SMV-SC3, SMV-SC7, and SMV-SC8 inoculatione6
- Comparator
- vector controle6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The full-length coding sequence of the MADS-box transcription factor GmCAL was cloned from the SMV-resistant soybean cultivar Kefeng 1”
e6“SMV content decreased significantly in GmCAL-overexpressing NN1138-2 plants after SMV-SC3, SMV-SC7, and SMV-SC8 inoculation in comparison with a vector control”
Claim · model-written summary
GmCAL is a positive regulatory transcription factor involved in soybean resistance to SMV.
- Taxon
- soybeanGlycine maxe7
- Interactor
- SMVe7pathogen
- Gene
- GmCALe7
- Outcome
- resistance to SMVe7
- Qualifier
- a positive regulatory transcription factore7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“These results confirm that GmCAL, a key regulator but not a specific SC8 resistance gene (Rsc8), is a positive regulatory transcription factor involved in soybean resistance to SMV”
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