Abstract
We have isolated two cDNA clones (cdc2-S5 and cdc2-S6) encoding p34cdc2 protein kinases, homologs of yeast cdc2/CDC28 genes, from a soybean nodule cDNA library. The two sequences share 90% sequence homology in the coding regions. The 5' and 3' noncoding regions are distinct from each other, however, indicating that at least two genes encode p34cdc2 protein kinases in soybean. Both sequences can rescue the cdc28 mutation in Saccharomyces cerevisiae but rescue it with different efficiency. Genomic Southern analysis showed the existence of two copies for each of these genes, which are not closely linked and are nonallelic. The relative expression level of the two soybean p34cdc2 genes varies in different tissues. Expression of cdc2-S5 is higher in roots and root nodules, whereas cdc2-S6 is more actively expressed in aerial tissues, indicating that regulation of these two p34cdc2 genes is coupled with plant developmental pathways. Expression of cdc2-S5 is, furthermore, enhanced after Rhizobium infection, whereas cdc2-S6 fails to respond, suggesting that cdc2-S5 plays a role in nodule initiation and organogenesis. This latter gene preferentially responds to auxin (alpha-naphthaleneacetic acid) treatment, indicating that phytohormones may be involved in the control of cell division mediated by Rhizobium infection. Thus, different p34cdc2 protein kinases may control cell division in different tissues in a multicellular organism and respond to different signals--e.g., phytohormones.
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Claims 9
Claim · model-written summary
The coding sequences share 90% homology.
- Taxon
- soybean
Glycine maxt0 - Outcome
- sequence homologye0
- Value
- 90%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“The two sequences share 90% sequence homology in the coding regions.”
Claim · model-written summary
The noncoding regions differ.
- Taxon
- soybeanGlycine maxt0
- Outcome
- 5' and 3' noncoding regionse0
- Direction
- distincte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“The 5' and 3' noncoding regions are distinct from each other”
Claim · model-written summary
Both sequences rescue the yeast mutation with different efficiencies.
- Taxon
- soybeanGlycine maxt0
- Gene
- cdc28e0
- Outcome
- rescue the cdc28 mutatione0
- Direction
- different efficiencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Both sequences can rescue the cdc28 mutation in Saccharomyces cerevisiae but rescue it with different efficiency.”
Claim · model-written summary
Each gene has two copies that are nonallelic and unlinked.
- Taxon
- soybeanGlycine maxt0
- Outcome
- two copies for each of these genese0
- Value
- two copiese0
- Qualifier
- not closely linked and are nonallelice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“two copies for each of these genes, which are not closely linked and are nonallelic.”
Claim · model-written summary
cdc2-S5 expression is higher in roots and nodules.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- roots and root nodulese0
- Gene
- cdc2-S5e0
- Outcome
- Expression of cdc2-S5e0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Expression of cdc2-S5 is higher in roots and root nodules”
Claim · model-written summary
cdc2-S6 is more active in aerial tissues.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- aerial tissuese0
- Gene
- cdc2-S6e0
- Outcome
- cdc2-S6 is more actively expressede0
- Direction
- more activelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“cdc2-S6 is more actively expressed in aerial tissues”
Claim · model-written summary
Rhizobium infection enhances cdc2-S5 expression.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Rhizobium infectione0
- Gene
- cdc2-S5e0
- Outcome
- Expression of cdc2-S5e0
- Direction
- enhancede0
RhizobiumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Expression of cdc2-S5 is, furthermore, enhanced after Rhizobium infection”
Claim · model-written summary
cdc2-S6 fails to respond to infection.
- Taxon
- soybeanGlycine maxt0
- Gene
- cdc2-S6e0
- Outcome
- cdc2-S6 fails to responde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“whereas cdc2-S6 fails to respond”
Claim · model-written summary
cdc2-S5 preferentially responds to auxin treatment.
- Taxon
- soybeanGlycine maxt0
- Treatment
- auxin (alpha-naphthaleneacetic acid) treatmente0
- Outcome
- preferentially respondse0
- Direction
- preferentially respondse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“This latter gene preferentially responds to auxin (alpha-naphthaleneacetic acid) treatment”
The same questions in other papers
infection · molecular: expression · Glycine max (soybean)Positive and negative results
18 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- no effect
- no effect
19 papers more in this group
See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.