Paper · 2021 · Molecules

Quantification of Flavonoids, Phenols and Antioxidant Potential from Dropped Citrus reticulata Blanco Fruits Influenced by Drying Techniques

Dinesh Kumar; Milind S. Ladaniya; Manju Gurjar; et al.

5
claims
11
verbatim quotes
30
citations
1
research areas
✓
Open access · cc-by

Abstract

Physiologically dropped immature Citrus reticulata Blanco fruits are regarded as waste and discarded in the citrus orchard but are a good source of bioactive compounds including flavonoids, antioxidants and total phenols. A study was undertaken to identify and quantify these bioactive compounds and to investigate the influence of different drying techniques, namely freeze drying and hot air oven drying, on flavonoids namely flavanone glycosides, antioxidant potential and total phenol content in immature dropped fruits of Citrus reticulata Blanco. Flavonoids were quantified in high-performance liquid chromatography (HPLC). The antioxidant activity were investigated with three assays azino-bis [3-ethylbenzthiazoline-6-sulfonic acid]) (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), Ferric Reducing Ability of Plasma (FRAP) and total phenol content was determined. Freeze dried samples of 12 and 14 mm size retained maximum hesperidin flavonoid content (27.03% and 27.20%) as compared to the hot air dried samples (17.99%) and retained higher phenolic content ranged from 50.54–54.19 mg GAEL−1. The antioxidant activity in freeze dried fruits was from 12.21–13.55 mM L−1 Trolox and 15.27–16.72 mM L−1 Trolox with ABTS, DPPH assay and FRAP values ranging from 7.31–9.07 mM L−1 Trolox. Significant positive correlation was found between the flavonoid hesperidin with antioxidant assays and total phenolic content (TPC). The results showed that waste citrus fruits can act as potential source of bioflavonoids, especially hesperidin, and antioxidants for pharmaceutical as well as nutraceutical industry.

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Freeze-dried 12 and 14 mm fruit samples retained maximum hesperidin content (27.03% and 27.20%) compared with hot-air-dried samples (17.99%).

Taxon
Citrus reticulata BlancoCitrus reticulatae0
Treatment
freeze dryinge2
Outcome
hesperidin flavonoid contente1
Direction
retained maximume1
Value
27.03% and 27.20%e1
Comparator
hot air dried samplese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus reticulata
Citrus reticulata · TaxonJoe Ravi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Physiologically dropped immature Citrus reticulata Blanco fruits”

✓ located verbatim in the source · abstract, characters 0–64 · show in text ↑
e1

“Freeze dried samples of 12 and 14 mm size retained maximum hesperidin flavonoid content (27.03% and 27.20%) as compared to the hot air dried samples (17.99%)”

✓ located verbatim in the source · abstract, characters 878–1035 · show in text ↑
e2

“namely freeze drying and hot air oven drying”

✓ located verbatim in the source · abstract, characters 361–405 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Freeze-dried fruits retained higher phenolic content, 50.54–54.19 mg GAEL−1.

Taxon
Citrus reticulata BlancoCitrus reticulatae0
Outcome
phenolic contente3
Direction
highere3
Value
50.54–54.19 mg GAEL−1e3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus reticulata
Citrus reticulata · TaxonJoe Ravi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Physiologically dropped immature Citrus reticulata Blanco fruits”

✓ located verbatim in the source · abstract, characters 0–64 · show in text ↑
e3

“retained higher phenolic content ranged from 50.54–54.19 mg GAEL−1”

✓ located verbatim in the source · abstract, characters 1040–1106 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Freeze-dried fruit antioxidant activity was 12.21–13.55 and 15.27–16.72 mM L−1 Trolox with the ABTS and DPPH assays.

Taxon
Citrus reticulata BlancoCitrus reticulatae0
Treatment
freeze driede4
Outcome
antioxidant activitye4
Value
12.21–13.55 mM L−1 Trolox and 15.27–16.72 mM L−1 Troloxe4
Qualifier
with ABTS, DPPH assaye4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus reticulata
Citrus reticulata · TaxonJoe Ravi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Physiologically dropped immature Citrus reticulata Blanco fruits”

✓ located verbatim in the source · abstract, characters 0–64 · show in text ↑
e4

“The antioxidant activity in freeze dried fruits was from 12.21–13.55 mM L−1 Trolox and 15.27–16.72 mM L−1 Trolox with ABTS, DPPH assay”

✓ located verbatim in the source · abstract, characters 1108–1242 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Freeze-dried fruit FRAP values were 7.31–9.07 mM L−1 Trolox.

Taxon
Citrus reticulata BlancoCitrus reticulatae0
Outcome
FRAP valuese5
Value
7.31–9.07 mM L−1 Troloxe5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus reticulata
Citrus reticulata · TaxonJoe Ravi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Physiologically dropped immature Citrus reticulata Blanco fruits”

✓ located verbatim in the source · abstract, characters 0–64 · show in text ↑
e5

“FRAP values ranging from 7.31–9.07 mM L−1 Trolox”

✓ located verbatim in the source · abstract, characters 1247–1295 · show in text ↑
Associationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Hesperidin correlated positively and significantly with the antioxidant assays and total phenolic content.

Taxon
Citrus reticulata BlancoCitrus reticulatae0
Compound
hesperidine6
Outcome
antioxidant assays and total phenolic content (TPC)e6
Direction
positivee6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus reticulata
Citrus reticulata · TaxonJoe Ravi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Physiologically dropped immature Citrus reticulata Blanco fruits”

✓ located verbatim in the source · abstract, characters 0–64 · show in text ↑
e6

“Significant positive correlation was found between the flavonoid hesperidin with antioxidant assays and total phenolic content (TPC)”

✓ located verbatim in the source · abstract, characters 1297–1429 · show in text ↑

processing · quality: phytochemicals · Citrus reticulata (tangerine)Positive and negative results

8 papers: 5 positive, 3 negative or no effect

4 papers in the same direction as this paper · 2 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. no effect

4 papers more in this group

See all 8 papers →

cold · quality: phytochemicals · Citrus reticulata (tangerine)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.