Abstract
Soil alkalization triggers ion toxicity and osmotic and alkaline (high pH) stresses in plants, damaging their growth and productivity. Therefore, we investigated whether priming with abscisic acid (ABA) increases the tolerance of alfalfa seedlings to alkaline stress, and then examined the underlying molecular mechanisms. Alfalfa seedlings were pretreated with ABA (10 μM) for 16 h and then subjected to alkaline stress using a 15 mM Na2CO3 solution (pH 10.87). Compared with the control, ABA pretreatment significantly alleviated leaf damage and improved the fresh weight, water content, and survival rate of alfalfa seedlings under alkaline conditions. Abscisic acid pretreatment reduced accumulation of reactive oxygen species (ROS), increased activities of the antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD), maintained higher ratios of K+/Na+, Ca2+/Na+, and Mg2+/Na+, and increased accumulation of proline. In addition, ABA upregulated the expression of genes involved in proline biosynthesis (P5CS) and the sequestration of Na+ in vacuoles (NHX1 and AVP) under alkaline conditions. Abscisic acid priming increased tolerance to alkaline stress by maintaining homeostasis of ROS and metal ions and upregulating osmoprotection and the expression of stress tolerance-related genes.
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Claims 9
Claim · model-written summary
ABA pretreatment alleviated leaf damage under alkaline conditions.
- Taxon
- alfalfa seedlings
Medicago sativae0 - Treatment
- ABA pretreatmente0
- Outcome
- leaf damagee0
- Direction
- alleviatede0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with the control, ABA pretreatment significantly alleviated leaf damage and improved the fresh weight, water content, and survival rate of alfalfa seedlings under alkaline conditions.”
Claim · model-written summary
ABA pretreatment improved fresh weight, water content and survival under alkaline conditions.
- Taxon
- alfalfa seedlingsMedicago sativae0
- Treatment
- ABA pretreatmente0
- Outcome
- fresh weight, water content, and survival ratee0
- Direction
- improvede0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with the control, ABA pretreatment significantly alleviated leaf damage and improved the fresh weight, water content, and survival rate of alfalfa seedlings under alkaline conditions.”
Claim · model-written summary
ABA pretreatment reduced reactive oxygen species accumulation.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Abscisic acid pretreatmente0
- Outcome
- accumulation of reactive oxygen species (ROS)e0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid pretreatment reduced accumulation of reactive oxygen species (ROS), increased activities of the antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD), maintained higher ratios of K+/Na+, Ca2+/Na+, and Mg2+/Na+, and increased accumulation of proline.”
t0“Alfalfa Seedlings (Medicago sativa L.)”
Claim · model-written summary
ABA pretreatment increased SOD and POD activities.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Abscisic acid pretreatmente0
- Outcome
- activities of the antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid pretreatment reduced accumulation of reactive oxygen species (ROS), increased activities of the antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD), maintained higher ratios of K+/Na+, Ca2+/Na+, and Mg2+/Na+, and increased accumulation of proline.”
t0“Alfalfa Seedlings (Medicago sativa L.)”
Claim · model-written summary
ABA pretreatment maintained higher K+/Na+, Ca2+/Na+ and Mg2+/Na+ ratios.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Abscisic acid pretreatmente0
- Outcome
- ratios of K+/Na+, Ca2+/Na+, and Mg2+/Na+e0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid pretreatment reduced accumulation of reactive oxygen species (ROS), increased activities of the antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD), maintained higher ratios of K+/Na+, Ca2+/Na+, and Mg2+/Na+, and increased accumulation of proline.”
t0“Alfalfa Seedlings (Medicago sativa L.)”
Claim · model-written summary
ABA pretreatment increased proline accumulation.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Abscisic acid pretreatmente0
- Outcome
- accumulation of prolinee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid pretreatment reduced accumulation of reactive oxygen species (ROS), increased activities of the antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD), maintained higher ratios of K+/Na+, Ca2+/Na+, and Mg2+/Na+, and increased accumulation of proline.”
t0“Alfalfa Seedlings (Medicago sativa L.)”
Claim · model-written summary
ABA upregulated P5CS expression under alkaline conditions.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- ABAe0
- Gene
- P5CSe0
- Outcome
- expression of genes involved in proline biosynthesis (P5CS)e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA upregulated the expression of genes involved in proline biosynthesis (P5CS) and the sequestration of Na+ in vacuoles (NHX1 and AVP) under alkaline conditions.”
t0“Alfalfa Seedlings (Medicago sativa L.)”
Claim · model-written summary
ABA upregulated NHX1 and AVP expression under alkaline conditions.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- ABAe0
- Gene
- NHX1 and AVPe0
- Outcome
- expression of genes involved in proline biosynthesis (P5CS) and the sequestration of Na+ in vacuoles (NHX1 and AVP)e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA upregulated the expression of genes involved in proline biosynthesis (P5CS) and the sequestration of Na+ in vacuoles (NHX1 and AVP) under alkaline conditions.”
t0“Alfalfa Seedlings (Medicago sativa L.)”
Claim · model-written summary
The authors attribute ABA priming tolerance to ROS and ion homeostasis, osmoprotection and gene expression.
- Taxon
- AlfalfaMedicago sativat0
- Outcome
- tolerance to alkaline stresse0
- Qualifier
- maintaining homeostasis of ROS and metal ions and upregulating osmoprotection and the expression of stress tolerance-related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid priming increased tolerance to alkaline stress by maintaining homeostasis of ROS and metal ions and upregulating osmoprotection and the expression of stress tolerance-related genes.”
t0“Alfalfa Seedlings (Medicago sativa L.)”
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.