Abstract
A 2-gram fresh weight inoculum of bromegrass (Bromus inermis Leyss. culture BG970) cell suspension culture treated with 7.5 x 10(-5) molar abscisic acid (ABA) for 7 days at 25 degrees C survived slow cooling to -60 degrees C. Over 80% of the cells in ABA treated cultures survived immersion in liquid N(2) after slow cooling to -40 or -60 degrees C. In contrast, a 6-gram fresh weight inoculum only attained a hardiness level of -28 degrees C after 5 days of ABA treatment. Ethanol (2 x 10(-2) molar) added to the culture medium at the time of ABA addition, inhibited the freezing tolerance of bromegrass cells by 25 degrees C. A 6-gram inoculum of both control and ABA treated bromegrass cells altered the pH of the medium more than a 2-gram inoculum. ABA inhibited the increase in fresh weight of bromegrass by 20% after 4 days. Both control and ABA (10(-4) molar) treated alfalfa cells (Medicago sativa L.) grown at 25 degrees C hardened from an initial LT(50) of -5 degrees C to an LT(50) of -23 degrees C by the third to fifth day after subculture. Thereafter, the cells dehardened but the ABA treated cells did not deharden to the same level as the control cells. ABA inhibited the increase in fresh weight of alfalfa by 50% after 5 days.
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Claims 9
Claim · model-written summary
ABA-treated bromegrass cells from a 2-gram inoculum survived cooling to -60 degrees C.
- Taxon
- bromegrasse0
- Material
- BG970e0
- Treatment
- 7.5 x 10(-5) molar abscisic acid (ABA)e0
- Outcome
- survived slow coolinge0
- Value
- -60 degrees Ce0
Not reportedComparatorDirection
Verbatim
e0“A 2-gram fresh weight inoculum of bromegrass (Bromus inermis Leyss. culture BG970) cell suspension culture treated with 7.5 x 10(-5) molar abscisic acid (ABA) for 7 days at 25 degrees C survived slow cooling to -60 degrees C”
Claim · model-written summary
Over 80% of ABA-treated bromegrass cells survived liquid nitrogen immersion after slow cooling.
- Taxon
- Bromus inermis Leyss
Bromus inermist0 - Treatment
- ABA treatede0
- Outcome
- survived immersion in liquid N(2)e0
- Value
- Over 80%e0
- Qualifier
- after slow cooling to -40 or -60 degrees Ce0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Over 80% of the cells in ABA treated cultures survived immersion in liquid N(2) after slow cooling to -40 or -60 degrees C”
t0“Bromus inermis Leyss”
Claim · model-written summary
A 6-gram inoculum reached only -28 degrees C hardiness after five days of ABA.
- Taxon
- Bromus inermis LeyssBromus inermist0
- Treatment
- ABA treatmente0
- Outcome
- hardiness levele0
- Direction
- only attainede0
- Value
- -28 degrees Ce0
- Qualifier
- 6-gram fresh weight inoculume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a 6-gram fresh weight inoculum only attained a hardiness level of -28 degrees C after 5 days of ABA treatment”
t0“Bromus inermis Leyss”
Claim · model-written summary
Ethanol addition inhibited bromegrass cell freezing tolerance by 25 degrees C.
- Taxon
- bromegrasse0
- Treatment
- Ethanol (2 x 10(-2) molar)e0
- Outcome
- freezing tolerancee0
- Direction
- inhibitede0
- Value
- 25 degrees Ce0
Not reportedComparator
Verbatim
e0“Ethanol (2 x 10(-2) molar) added to the culture medium at the time of ABA addition, inhibited the freezing tolerance of bromegrass cells by 25 degrees C”
Claim · model-written summary
A 6-gram inoculum changed medium pH more than a 2-gram inoculum in both control and ABA-treated bromegrass cultures.
- Taxon
- bromegrasse0
- Treatment
- ABA treatede0
- Outcome
- pH of the mediume0
- Direction
- moree0
- Comparator
- a 2-gram inoculume0
Not reportedMaterial
Verbatim
e0“A 6-gram inoculum of both control and ABA treated bromegrass cells altered the pH of the medium more than a 2-gram inoculum”
Claim · model-written summary
ABA inhibited bromegrass fresh-weight increase by 20% after four days.
Not reportedComparator
Verbatim
e0“ABA inhibited the increase in fresh weight of bromegrass by 20% after 4 days”
Claim · model-written summary
Control and ABA-treated alfalfa cells hardened from -5 to -23 degrees C by days three to five.
- Taxon
- alfalfa
Medicago sativae0 - Treatment
- ABA (10(-4) molar)e0
- Outcome
- LT(50)e0
- Direction
- hardenede0
- Value
- -23 degrees Ce0
- Qualifier
- an initial LT(50) of -5 degrees Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both control and ABA (10(-4) molar) treated alfalfa cells (Medicago sativa L.) grown at 25 degrees C hardened from an initial LT(50) of -5 degrees C to an LT(50) of -23 degrees C by the third to fifth day after subculture”
Claim · model-written summary
ABA-treated alfalfa cells dehardened less than control cells later in culture.
Verbatim
e0“Thereafter, the cells dehardened but the ABA treated cells did not deharden to the same level as the control cells”
Claim · model-written summary
ABA inhibited the fresh-weight increase of alfalfa by 50% after five days.
- Taxon
- alfalfaMedicago sativae0
- Treatment
- ABAe0
- Outcome
- increase in fresh weighte0
- Direction
- inhibitede0
- Value
- 50%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA inhibited the increase in fresh weight of alfalfa by 50% after 5 days”
The same questions in other papers
(+)-abscisic acid · growth · Medicago sativa (alfalfa)Positive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.