Abstract
Abstract Phosphorus (P) is an essential element for the growth and development of plants. Soybean (Glycine max) is an important food crop that is grown worldwide. Soybean yield is significantly affected by P deficiency in the soil. To investigate the molecular factors that determine the response and tolerance at low-P in soybean, we conducted a comparative proteomics study of a genotype with low-P tolerance (Liaodou 13, L13) and a genotype with low-P sensitivity (Tiefeng 3, T3) in a paper culture experiment with three P treatments, i.e. P-free (0 mmol·L−1), low-P (0.05 mmol·L−1) and normal-P (0.5 mmol·L−1). A total of 4126 proteins were identified in roots of the two genotypes. Increased numbers of differentially expressed proteins (DEPs) were obtained from low-P to P-free conditions compared to the normal-P treatment. All DEPs obtained in L13 (660) were upregulated in response to P deficiency, while most DEPs detected in T3 (133) were downregulated under P deficiency. Important metabolic pathways such as oxidative phosphorylation, glutathione metabolism and carbon metabolism were suppressed in T3, which could have affected the survival of the plants in P-limited soil. In contrast, L13 increased the metabolic activity in the 2-oxocarboxylic acid metabolism, carbon metabolism, glycolysis, biosynthesis of amino acids, pentose phosphatase, oxidative phosphorylation, other types of O-glycan biosynthesis and riboflavin metabolic pathways in order to maintain normal plant growth under P deficiency. Three key proteins I1KW20 (prohibitins), I1K3U8 (alpha-amylase inhibitors) and C6SZ93 (alpha-amylase inhibitors) were suggested as potential biomarkers for screening soybean genotypes with low-P tolerance. Overall, this study provides new insights into the response and tolerance to P deficiency in soybean.
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Claims 8
Claim · model-written summary
All differentially expressed proteins in L13 were upregulated under phosphorus deficiency.
- Taxon
- soybean
Glycine maxt0 - Material
- Liaodou 13, L13e3
- Treatment
- P deficiencye1
- Outcome
- All DEPs obtained in L13 (660)e1
- Direction
- upregulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean genotypes”
e1“All DEPs obtained in L13 (660) were upregulated in response to P deficiency”
e3“a genotype with low-P tolerance (Liaodou 13, L13)”
Claim · model-written summary
Most differentially expressed proteins in T3 were downregulated under phosphorus deficiency.
- Taxon
- soybeanGlycine maxt0
- Material
- Tiefeng 3, T3e4
- Treatment
- P deficiencye2
- Outcome
- most DEPs detected in T3 (133)e2
- Direction
- downregulatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean genotypes”
e2“most DEPs detected in T3 (133) were downregulated under P deficiency”
e4“a genotype with low-P sensitivity (Tiefeng 3, T3)”
Claim · model-written summary
More differentially expressed proteins were obtained under P-free conditions than under normal-P treatment.
- Taxon
- soybeanGlycine maxt0
- Treatment
- P-freee5
- Outcome
- differentially expressed proteins (DEPs)e5
- Direction
- Increasede5
- Comparator
- normal-P treatmente5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean genotypes”
e5“Increased numbers of differentially expressed proteins (DEPs) were obtained from low-P to P-free conditions compared to the normal-P treatment”
Claim · model-written summary
Oxidative phosphorylation, glutathione metabolism and carbon metabolism were suppressed in T3 under P limitation.
- Taxon
- soybeanGlycine maxt0
- Material
- T3e4
- Treatment
- P-limited soile6
- Outcome
- Important metabolic pathways such as oxidative phosphorylation, glutathione metabolism and carbon metabolisme6
- Direction
- suppressede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean genotypes”
e6“Important metabolic pathways such as oxidative phosphorylation, glutathione metabolism and carbon metabolism were suppressed in T3, which could have affected the survival of the plants in P-limited soil”
e4“a genotype with low-P sensitivity (Tiefeng 3, T3)”
Claim · model-written summary
L13 increased 2-oxocarboxylic acid metabolism, carbon metabolism and glycolysis under P deficiency.
- Taxon
- soybeanGlycine maxt0
- Material
- L13e7
- Treatment
- P deficiencye7
- Outcome
- 2-oxocarboxylic acid metabolism, carbon metabolism, glycolysise7
- Direction
- increasede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean genotypes”
e7“L13 increased the metabolic activity in the 2-oxocarboxylic acid metabolism, carbon metabolism, glycolysis, biosynthesis of amino acids, pentose phosphatase, oxidative phosphorylation, other types of O-glycan biosynthesis and riboflavin metabolic pathways in order to maintain normal plant growth under P deficiency”
Claim · model-written summary
L13 increased biosynthesis of amino acids, pentose phosphatase and oxidative phosphorylation under P deficiency.
- Taxon
- soybeanGlycine maxt0
- Material
- L13e7
- Treatment
- P deficiencye7
- Outcome
- biosynthesis of amino acids, pentose phosphatase, oxidative phosphorylatione7
- Direction
- increasede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean genotypes”
e7“L13 increased the metabolic activity in the 2-oxocarboxylic acid metabolism, carbon metabolism, glycolysis, biosynthesis of amino acids, pentose phosphatase, oxidative phosphorylation, other types of O-glycan biosynthesis and riboflavin metabolic pathways in order to maintain normal plant growth under P deficiency”
Claim · model-written summary
L13 increased other types of O-glycan biosynthesis and riboflavin metabolism under P deficiency.
- Taxon
- soybeanGlycine maxt0
- Material
- L13e7
- Treatment
- P deficiencye7
- Outcome
- other types of O-glycan biosynthesis and riboflavin metabolic pathwayse7
- Direction
- increasede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean genotypes”
e7“L13 increased the metabolic activity in the 2-oxocarboxylic acid metabolism, carbon metabolism, glycolysis, biosynthesis of amino acids, pentose phosphatase, oxidative phosphorylation, other types of O-glycan biosynthesis and riboflavin metabolic pathways in order to maintain normal plant growth under P deficiency”
Claim · model-written summary
Three proteins (I1KW20, I1K3U8 and C6SZ93) are suggested as potential biomarkers for screening soybean genotypes with low-P tolerance.
- Taxon
- soybeanGlycine maxt0
- Gene
- I1KW20 (prohibitins), I1K3U8 (alpha-amylase inhibitors) and C6SZ93 (alpha-amylase inhibitors)e1
- Outcome
- potential biomarkers for screening soybean genotypes with low-P tolerancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean genotypes”
e1“Three key proteins I1KW20 (prohibitins), I1K3U8 (alpha-amylase inhibitors) and C6SZ93 (alpha-amylase inhibitors) were suggested as potential biomarkers for screening soybean genotypes with low-P tolerance”
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See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.