Abstract
Carboxylate efflux from roots is a crucial and differential response of soybean genotypes to low phosphorus (P) stress. Exudation of carboxylic acids including oxalate, citrate, succinate and fumarate was induced under low P stress, particularly in P-efficient soybean genotypes. Enhancement of root length, surface area and volume further improved P acquisition under low P stress. To understand the molecular basis of carboxylate efflux under low P stress, the root proteome of contrasting genotypes (P-efficient: EC-232019 and P-inefficient: EC-113396) was compared. Among a total of 325 spots, 105 (32%) were differentially abundant proteins (DAPs) between sufficient (250 µM) and low P (4 µM) levels. Abundance of 44 (14%) proteins decreased by more than two-fold under low P stress, while 61 (19%) proteins increased by more than two-fold. Protein identification and annotation revealed that the DAPs were involved in a myriad of functions including carboxylic acid synthesis, carbohydrate, protein and lipid metabolism. Proteins with significant abundance included malate dehydrogenase, isocitrate dehydrogenase, phosphoglucomutase, phosphoglycerate mutase, fructokinase, enolase, phosphoglycerate kinase, triosephosphate isomerase, alcohol dehydrogenase, glucan water dikinase, glutamine synthetase and argininosuccinate lyase. Inferences from proteomic analysis suggests the crosstalk between various metabolic pathways implicated in conferring superior P acquisition efficiency under stress.
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Claims 8
Claim · model-written summary
Low phosphorus induced root exudation of oxalate, citrate, succinate and fumarate, especially in phosphorus-efficient soybean genotypes.
- Taxon
- soybean
Glycine maxt0 - Material
- P-efficient soybean genotypese0
- Treatment
- low P stresse0
- Outcome
- Exudation of carboxylic acids including oxalate, citrate, succinate and fumaratee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exudation of carboxylic acids including oxalate, citrate, succinate and fumarate was induced under low P stress, particularly in P-efficient soybean genotypes.”
t0“Soybean”
Claim · model-written summary
Enhanced root length, surface area and volume improved phosphorus acquisition under low phosphorus stress.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- roote0
- Outcome
- P acquisitione0
- Qualifier
- Enhancement of root length, surface area and volumee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enhancement of root length, surface area and volume further improved P acquisition under low P stress.”
t0“Soybean”
Claim · model-written summary
Of 325 protein spots, 105 were differentially abundant between sufficient and low phosphorus.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- Roott0
- Treatment
- low P (4 µM)e0
- Outcome
- differentially abundant proteins (DAPs)e0
- Value
- 105 (32%)e0
- Comparator
- sufficient (250 µM)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among a total of 325 spots, 105 (32%) were differentially abundant proteins (DAPs) between sufficient (250 µM) and low P (4 µM) levels.”
t0“Soybean Root”
Claim · model-written summary
Under low phosphorus, abundance of 44 proteins decreased more than twofold.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- low P stresse0
- Outcome
- proteinse0
- Direction
- decreasede0
- Value
- 44 (14%)e0
- Qualifier
- more than two-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abundance of 44 (14%) proteins decreased by more than two-fold under low P stress”
t0“Soybean”
Claim · model-written summary
Under low phosphorus, abundance of 61 proteins increased more than twofold.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- low Pt1
- Outcome
- proteinst1
- Direction
- increasede0
- Value
- 61 (19%)e0
- Qualifier
- more than two-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while 61 (19%) proteins increased by more than two-fold.”
t0“Soybean”
t1“under low P stress, while 61 (19%) proteins increased by more than two-fold.”
Claim · model-written summary
Differentially abundant proteins covered carboxylic acid synthesis and carbohydrate, protein and lipid metabolism.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- carboxylic acid synthesis, carbohydrate, protein and lipid metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protein identification and annotation revealed that the DAPs were involved in a myriad of functions including carboxylic acid synthesis, carbohydrate, protein and lipid metabolism.”
t0“Soybean”
Claim · model-written summary
Proteins with significant abundance included enzymes of carboxylate and central metabolism.
- Taxon
- SoybeanGlycine maxt0
- Gene
- malate dehydrogenasee0
- Outcome
- significant abundancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proteins with significant abundance included malate dehydrogenase, isocitrate dehydrogenase, phosphoglucomutase, phosphoglycerate mutase, fructokinase, enolase, phosphoglycerate kinase, triosephosphate isomerase, alcohol dehydrogenase, glucan water dikinase, glutamine synthetase and argininosuccinate lyase.”
t0“Soybean”
Claim · model-written summary
Proteomic results suggest crosstalk among metabolic pathways contributing to superior phosphorus acquisition under stress.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- P acquisition efficiencye0
- Qualifier
- crosstalk between various metabolic pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inferences from proteomic analysis suggests the crosstalk between various metabolic pathways implicated in conferring superior P acquisition efficiency under stress.”
t0“Soybean”
The same questions in other papers
phosphorus · molecular: metabolites · Glycine max (soybean)Positive and negative results
27 papers in the same direction as this paper · 10 in the opposite direction
- negative
- no effect
- negative
34 papers more in this group
See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.