Abstract
The enzyme 12-oxo-phytodienoic acid reductase (OPR) is important in the jasmonic acid (JA) biosynthesis pathway and thus plays a vital role in plant defence. However, systematic and comprehensive analyses of OPR genes in watermelon and their roles in defence responses are extremely limited. The physicochemical properties, phylogenetic tree, gene structure and cis-acting elements of watermelon OPR genes were analysed using bioinformatics, and qRT-PCR and RNA-Seq were applied to assay expression of OPR genes in watermelon. A total of five OPR family genes were identified in watermelon, which were unevenly distributed across the four chromosomes. Phylogenetic analysis assigned OPR members from different plant species to five subfamilies (OPRI-OPRV). The motif compositions of OPR members were relatively conserved. Expression analysis using qRT-PCR revealed that ClOPR genes, except for ClOPR5, were highly expressed in the flower and fruit. RNA-seq analysis showed that the ClOPR genes had different expression patterns during flesh and rind development. Furthermore, the ClOPR genes, particularly ClOPR2 and ClOPR4, were significantly upregulated by exogenous JA, salicylic acid (SA) and ethylene (ET) treatments. In addition, red light induced expression of ClOPR2 and ClOPR4 in leaves and roots of root-knot nematode (RKN)-infected watermelon plants, suggesting their involvement in red light-induced defence against RKN. These results provide a theoretical basis for elucidating the diverse functions of OPR family genes in watermelon.
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Claims 7
Claim · model-written summary
Five OPR genes were found on four watermelon chromosomes.
- Taxon
- watermelon
Citrullus lanatuse0 - Gene
- OPR family genese0
- Outcome
- unevenly distributed across the four chromosomese0
- Value
- fivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of five OPR family genes were identified in watermelon, which were unevenly distributed across the four chromosomes.”
Claim · model-written summary
Phylogenetic analysis assigned OPR members to five subfamilies.
Verbatim
e0“Phylogenetic analysis assigned OPR members from different plant species to five subfamilies (OPRI-OPRV).”
Claim · model-written summary
OPR motif compositions were relatively conserved.
- Taxon
- watermelonCitrullus lanatust0
- Gene
- OPR memberse0
- Outcome
- motif compositionse0
- Direction
- relatively conservede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The motif compositions of OPR members were relatively conserved.”
t0“watermelon”
Claim · model-written summary
ClOPR genes other than ClOPR5 were highly expressed in flower and fruit.
- Taxon
- watermelonCitrullus lanatust0
- Organ / stage
- flower and fruite0
- Gene
- ClOPR genese0
- Outcome
- highly expressede0
- Qualifier
- except for ClOPR5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ClOPR genes, except for ClOPR5, were highly expressed in the flower and fruit.”
t0“watermelon”
Claim · model-written summary
ClOPR genes showed differing expression across flesh and rind development.
- Taxon
- watermelonCitrullus lanatust0
- Organ / stage
- flesh and rind developmente0
- Gene
- ClOPR genese0
- Outcome
- different expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ClOPR genes had different expression patterns during flesh and rind development.”
t0“watermelon”
Claim · model-written summary
Exogenous JA, SA and ET significantly upregulated ClOPR genes, especially ClOPR2 and ClOPR4.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- exogenous JA, salicylic acid (SA) and ethylene (ET) treatmentse0
- Gene
- ClOPR2 and ClOPR4e0
- Outcome
- ClOPR genese0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ClOPR genes, particularly ClOPR2 and ClOPR4, were significantly upregulated by exogenous JA, salicylic acid (SA) and ethylene (ET) treatments.”
t0“watermelon”
Claim · model-written summary
Red light induced ClOPR2 and ClOPR4 expression in leaves and roots of nematode-infected plants.
- Taxon
- watermelonCitrullus lanatuse0
- Organ / stage
- leaves and rootse0
- Treatment
- red lighte0
- Interactor
- root-knot nematode (RKN)
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=60)
Meloidogynee0pathogen - Gene
- ClOPR2 and ClOPR4e0
- Outcome
- expression of ClOPR2 and ClOPR4e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=500)
Verbatim
e0“red light induced expression of ClOPR2 and ClOPR4 in leaves and roots of root-knot nematode (RKN)-infected watermelon plants”
The same questions in other papers
jasmonic acid · molecular: expression · Citrullus lanatus (watermelon)Positive and negative results
2 reporting no difference from an alternative
- no effect
- no effect
salicylic acid · molecular: expression · Citrullus lanatus (watermelon)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →ethene · molecular: expression · Citrullus lanatus (watermelon)Results agree
1 paper in the same direction as this paper
- positive
light · Meloidogyne · Citrullus lanatus (watermelon)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.