Abstract
RNA silencing, a core part of plants' antiviral defence, requires the ARGONAUTE, DICER-like, and RNA-dependent RNA polymerase proteins. However, how these proteins contribute to watermelon's RNA interference (RNAi) pathway response to cucumber green mottle mosaic virus (CGMMV) has not been characterized. Here, we identify seven ClAGO, four ClDCL, and 11 ClRDR genes in watermelon and analyse their expression profiles when infected with CGMMV. ClAGO1 and ClAGO5 expression levels were highly induced by CGMMV infection. The results of ClAGO1 and ClAGO5 overexpression and silencing experiments suggest that these genes play central roles in watermelon's antiviral defence. Furthermore, co-immunoprecipitation and bimolecular fluorescence complementation experiments showed that ClAGO1 interacts with ClAGO5 in vivo, suggesting that ClAGO1 and ClAGO5 co-regulate watermelon defence against CGMMV infection. We also identified the ethylene response factor (ERF) binding site in the promoters of the ClAGO1 and ClAGO5 genes, and ethylene (ETH) treatment significantly increased ClAGO5 expression. Two ERF genes (Cla97C08G147180 and Cla97C06G122830) closely related to ClAGO5 expression were identified using co-expression analysis. Subcellular localization revealed that two ERFs and ClAGO5 predominantly localize at the nucleus, suggesting that enhancement of resistance to CGMMV by ETH is probably achieved through ClAGO5 but not ClAGO1. Our findings reveal aspects of the mechanisms underlying RNA silencing in watermelon against CGMMV.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 13
Claim · model-written summary
Seven ClAGO genes were identified in watermelon.
- Taxon
- watermelon
Citrullus lanatuse0 - Gene
- ClAGOe0
- Outcome
- ClAGOe0
- Value
- sevene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identify seven ClAGO, four ClDCL, and 11 ClRDR genes in watermelon”
Claim · model-written summary
Four ClDCL genes were identified in watermelon.
- Taxon
- watermelonCitrullus lanatuse0
- Gene
- ClDCLe0
- Outcome
- ClDCLe0
- Value
- foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identify seven ClAGO, four ClDCL, and 11 ClRDR genes in watermelon”
Claim · model-written summary
Eleven ClRDR genes were identified in watermelon.
- Taxon
- watermelonCitrullus lanatuse0
- Gene
- ClRDRe0
- Outcome
- ClRDRe0
- Value
- 11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identify seven ClAGO, four ClDCL, and 11 ClRDR genes in watermelon”
Claim · model-written summary
CGMMV infection highly induced ClAGO1 expression.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- CGMMV infectione0
- Interactor
- CGMMVCucumber green mottle mosaic viruse0pathogen
- Gene
- ClAGO1e0
- Outcome
- ClAGO1e0
- Direction
- highly inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ClAGO1 and ClAGO5 expression levels were highly induced by CGMMV infection”
t0“watermelon”
Claim · model-written summary
CGMMV infection highly induced ClAGO5 expression.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- CGMMV infectione0
- Interactor
- CGMMVCucumber green mottle mosaic viruse0pathogen
- Gene
- ClAGO5e0
- Outcome
- ClAGO5 expression levelse0
- Direction
- highly inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ClAGO1 and ClAGO5 expression levels were highly induced by CGMMV infection”
t0“watermelon”
Claim · model-written summary
ClAGO1 has a central role in antiviral defense according to overexpression and silencing.
- Taxon
- watermelonCitrullus lanatuse0
- Gene
- ClAGO1e0
- Outcome
- antiviral defencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results of ClAGO1 and ClAGO5 overexpression and silencing experiments suggest that these genes play central roles in watermelon's antiviral defence”
Claim · model-written summary
ClAGO5 has a central role in antiviral defense according to overexpression and silencing.
- Taxon
- watermelonCitrullus lanatuse0
- Gene
- ClAGO5e0
- Outcome
- antiviral defencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results of ClAGO1 and ClAGO5 overexpression and silencing experiments suggest that these genes play central roles in watermelon's antiviral defence”
Claim · model-written summary
ClAGO1 interacted with ClAGO5 in vivo.
- Taxon
- watermelonCitrullus lanatust0
- Gene
- ClAGO1e0
- Outcome
- interacts with ClAGO5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ClAGO1 interacts with ClAGO5 in vivo”
t0“watermelon”
Claim · model-written summary
ERF binding sites were identified in promoters of ClAGO1 and ClAGO5.
- Taxon
- watermelonCitrullus lanatust0
- Gene
- ClAGO1 and ClAGO5e0
- Outcome
- ethylene response factor (ERF) binding sitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified the ethylene response factor (ERF) binding site in the promoters of the ClAGO1 and ClAGO5 genes”
t0“watermelon”
Claim · model-written summary
Ethylene treatment significantly increased ClAGO5 expression.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- ethylene (ETH) treatmente0
- Gene
- ClAGO5e0
- Outcome
- ClAGO5 expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene (ETH) treatment significantly increased ClAGO5 expression”
t0“watermelon”
Claim · model-written summary
Two ERF genes were closely related to ClAGO5 expression.
- Taxon
- watermelonCitrullus lanatust0
- Gene
- Cla97C08G147180 and Cla97C06G122830e0
- Outcome
- ClAGO5 expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two ERF genes (Cla97C08G147180 and Cla97C06G122830) closely related to ClAGO5 expression were identified using co-expression analysis”
t0“watermelon”
Claim · model-written summary
Both ERFs and ClAGO5 predominantly localized in the nucleus.
- Taxon
- watermelonCitrullus lanatust0
- Gene
- ClAGO5e0
- Outcome
- predominantly localize at the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two ERFs and ClAGO5 predominantly localize at the nucleus”
t0“watermelon”
Claim · model-written summary
Authors inferred that ETH enhanced CGMMV resistance through ClAGO5 rather than ClAGO1.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- ETHe0
- Interactor
- CGMMVCucumber green mottle mosaic viruse0pathogen
- Gene
- ClAGO5e0
- Outcome
- resistance to CGMMVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhancement of resistance to CGMMV by ETH is probably achieved through ClAGO5 but not ClAGO1”
t0“watermelon”
The same questions in other papers
ethene · molecular: expression · Citrullus lanatus (watermelon)Results agree
1 paper in the same direction as this paper
- positive
Cucumis sativus · Cucumber green mottle mosaic virus · Citrullus lanatus (watermelon)Results agree
1 paper in the same direction as this paper
- positive
infection · Cucumber green mottle mosaic virus · Citrullus lanatus (watermelon)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.