Abstract
BACKGROUND: Plants are easily affected by temperature variations, and high temperature (heat stress) and low temperature (cold stress) will lead to poor plant development and reduce crop yields. Therefore, it is very important to identify resistance genes for improving the ability of plants to resist heat stress or cold stress by using modern biotechnology. Members of the C-repeat binding factor/Dehydration responsive element-binding 1 (CBF/DREB1) protein family are related to the stress resistance of many plant species. These proteins affect the growth and development of plants and play vital roles during environmental stress (cold, heat, drought, salt, etc.). In this study, we identified CBF/DREB1 genes from 43 plant species (including algae, moss, ferns, gymnosperms, angiosperms) by using bioinformatic methods to clarify the characteristics of the CBF/DREB1 protein family members and their functions in potato under heat and cold stresses. RESULTS: In this study, we identified 292 CBF/DREB1 proteins from 43 plant species. However, no CBF/DREB1 protein was found in algae, moss, ferns, or gymnosperms; members of this protein family exist only in angiosperms. Phylogenetic analysis of all the CBF/DREB1 proteins revealed five independent groups. Among them, the genes of group I do not exist in eudicots and are found only in monocots, indicating that these genes have a special effect on monocots. The analysis of motifs, gene duplication events, and the expression data from the PGSC website revealed the gene structures, evolutionary relationships, and expression patterns of the CBF/DREB1 proteins. In addition, analysis of the transcript levels of the 8 CBF/DREB1 genes in potato (Solanum tuberosum) under low-temperature and high-temperature stresses showed that these genes were related to temperature stresses. In particular, the expression levels of StCBF3 and StCBF4 in the leaves, stems, and roots significantly increased under high-temperature conditions, which suggested that StCBF3 and StCBF4 may be closely related to heat tolerance in potato. CONCLUSION: Overall, members of the CBF/DREB1 protein family exist only in angiosperms and plays an important role in the growth and development of plants. In addition, the CBF/DREB1 protein family is related to the heat and cold resistance of potato. Our research revealed the evolution of the CBF/DREB1 family, and is useful for studying the precise functions of the CBF/DREB1 proteins when the plants are developing and are under temperature stress.
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Claims 8
Claim · model-written summary
The survey identified 292 CBF/DREB1 proteins in 43 plant species.
Verbatim
e0“we identified 292 CBF/DREB1 proteins from 43 plant species”
Claim · model-written summary
CBF/DREB1 proteins were absent from algae, moss, ferns, and gymnosperms and confined to angiosperms.
- Taxon
- angiospermsMagnoliopsidae0
- Gene
- CBF/DREB1 proteine0
- Outcome
- members of this protein family exist only in angiospermse0
Verbatim
e0“no CBF/DREB1 protein was found in algae, moss, ferns, or gymnosperms; members of this protein family exist only in angiosperms”
Claim · model-written summary
Phylogenetic analysis resolved five CBF/DREB1 groups.
Verbatim
e0“Phylogenetic analysis of all the CBF/DREB1 proteins revealed five independent groups”
t0“43 plant species”
Claim · model-written summary
Group I genes occurred in monocots but not eudicots.
- Taxon
- monocotsLiliopsidae0
- Gene
- genes of group Ie0
- Outcome
- found only in monocotse0
Verbatim
e0“the genes of group I do not exist in eudicots and are found only in monocots”
Claim · model-written summary
Motif, duplication, and expression analyses described CBF/DREB1 gene structures, relationships, and expression patterns.
Verbatim
e0“The analysis of motifs, gene duplication events, and the expression data from the PGSC website revealed the gene structures, evolutionary relationships, and expression patterns of the CBF/DREB1 proteins”
t0“43 plant species”
Claim · model-written summary
Eight potato CBF/DREB1 genes had transcript responses related to low and high temperature stress.
- Taxon
- potato (Solanum tuberosum)
Solanum tuberosume0 - Treatment
- low-temperature and high-temperature stressese0
- Gene
- CBF/DREB1 genese0
- Outcome
- transcript levelse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcript levels of the 8 CBF/DREB1 genes in potato (Solanum tuberosum) under low-temperature and high-temperature stresses showed that these genes were related to temperature stresses”
Claim · model-written summary
StCBF3 expression increased significantly in potato leaves, stems, and roots at high temperature.
- Taxon
- Solanum tuberosumSolanum tuberosumt0
- Organ / stage
- leaves, stems, and rootse0
- Treatment
- high-temperature conditionse0
- Gene
- StCBF3e0
- Outcome
- expression levels of StCBF3e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression levels of StCBF3 and StCBF4 in the leaves, stems, and roots significantly increased under high-temperature conditions”
t0“Solanum tuberosum”
Claim · model-written summary
StCBF4 expression increased significantly in potato leaves, stems, and roots at high temperature.
- Taxon
- Solanum tuberosumSolanum tuberosumt0
- Organ / stage
- leaves, stems, and rootse0
- Treatment
- high-temperature conditionse0
- Gene
- StCBF4e0
- Outcome
- expression levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression levels of StCBF3 and StCBF4 in the leaves, stems, and roots significantly increased under high-temperature conditions”
t0“Solanum tuberosum”
The same questions in other papers
heat · molecular: expression · Solanum tuberosum (potato)Positive and negative results
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- negative
- no effect
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.