Abstract
Storage of potato (Solanum tuberosum) tubers at 4 degrees C is associated with the accumulation of several transcripts. DNA sequence analysis of cDNA clone CI21, which corresponds to one of the cold-induced transcripts, revealed high homology to transcripts of tomato (Lycopersicon esculentum) and wild potato (Solanum chacoense) induced by ripening and water stress. Two homologous, nonallelic genes, ci21A and ci21B, were isolated and sequenced. Northern blot analysis showed that CI21 transcripts were present at the highest levels in cold-stored tubers, at lower levels in stems and roots, and at the lowest levels in leaves and tubers stored at room temperature. Treatment with abscisic acid, heat, and a high concentration of salt had no marked effect on CI21 transcript levels in tubers and leaves. Drought was the only stress treatment that induced CI21 transcripts in leaves, but it did not do so in tubers. Western blot analysis detected CI21 protein only in tubers. Chimeric gene constructs between the putative ci21A promoter region and the uidA reporter gene were tested in transgenic potato plants for induction of beta-glucuronidase activity by low temperature. A 2-fold increase of beta-glucuronidase activity in response to tuber storage at 4 degrees C was observed for fragments between 380 and 2000 bp of the ci21A promoter region.
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Claims 8
Claim · model-written summary
CI21 cDNA showed high homology to tomato and wild potato stress-related transcripts.
- Taxon
- potato
Solanum tuberosumt0 - Gene
- CI21e0
- Outcome
- high homology to transcripts of tomato (Lycopersicon esculentum) and wild potato (Solanum chacoense)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA sequence analysis of cDNA clone CI21, which corresponds to one of the cold-induced transcripts, revealed high homology to transcripts of tomato (Lycopersicon esculentum) and wild potato (Solanum chacoense) induced by ripening and water stress”
t0“Potato”
Claim · model-written summary
Two homologous nonallelic potato genes, ci21A and ci21B, were isolated and sequenced.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- ci21A and ci21Be0
- Outcome
- isolated and sequencede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two homologous, nonallelic genes, ci21A and ci21B, were isolated and sequenced”
t0“Potato”
Claim · model-written summary
CI21 transcripts were highest in cold-stored tubers, lower in stems and roots, and lowest in leaves and room-temperature tubers.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- tuberse0
- Treatment
- cold-storede0
- Gene
- CI21e0
- Outcome
- CI21 transcriptse0
- Direction
- highest levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CI21 transcripts were present at the highest levels in cold-stored tubers, at lower levels in stems and roots, and at the lowest levels in leaves and tubers stored at room temperature”
t0“Potato”
Claim · model-written summary
Abscisic acid, heat, and high salt had no marked effect on CI21 transcripts in tubers and leaves.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- abscisic acid, heat, and a high concentration of salte0
- Gene
- CI21e0
- Outcome
- CI21 transcript levelse0
- Direction
- no marked effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with abscisic acid, heat, and a high concentration of salt had no marked effect on CI21 transcript levels in tubers and leaves”
t0“Potato”
Claim · model-written summary
Drought induced CI21 transcripts in leaves.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Droughte0
- Gene
- CI21e0
- Outcome
- CI21 transcripts in leavese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought was the only stress treatment that induced CI21 transcripts in leaves, but it did not do so in tubers”
t0“Potato”
Claim · model-written summary
Drought did not induce CI21 transcripts in tubers.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- tuberse0
- Treatment
- Droughte0
- Gene
- CI21e0
- Outcome
- CI21 transcriptse0
- Direction
- did not do soe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought was the only stress treatment that induced CI21 transcripts in leaves, but it did not do so in tubers”
t0“Potato”
Claim · model-written summary
CI21 protein was detected only in tubers.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- CI21 proteine0
- Outcome
- detected CI21 protein only in tuberse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Western blot analysis detected CI21 protein only in tubers”
t0“Potato”
Claim · model-written summary
Tuber storage at 4 degrees C doubled reporter activity from 380 to 2000 bp ci21A promoter fragments.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- tuber storage at 4 degrees Ce0
- Gene
- ci21Ae0
- Outcome
- beta-glucuronidase activitye0
- Direction
- increasee0
- Value
- 2-folde0
- Qualifier
- fragments between 380 and 2000 bpe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 2-fold increase of beta-glucuronidase activity in response to tuber storage at 4 degrees C was observed for fragments between 380 and 2000 bp of the ci21A promoter region”
t0“Potato”
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.