Abstract
We report the inhibitory effect of peptide extracts obtained from seven medicinal plants against a causative agent of late blight disease Phytophthora infestans. We find that all the extracts possess inhibitory activity toward the zoospores output, zoosporangium germination, and the development of P. infestans on potato disc tubers at different quantitative levels. Based on the biological effects detected, an extract of common horsetail (Equisetum arvense) biomass is recognized as the most effective and is selected for further structural analysis. We perform a combination of amino acid analysis and MALDI-TOF mass spectrometry, which reveal the presence of Asn/Asp- and Gln/Glu-rich short peptides with molecular masses in the range of 500–900 Da and not exceeding 1500 Da as the maximum. Analytical anion-exchange HPLC is successfully applied for separation of the peptide extract from common horsetail (E. arvense). We collect nine dominant components that are combined in two groups with differences in retention times. The N-terminal amino acid sequence of the prevalent compounds after analytical ion-exchange HPLC allows us to identify them as peptide fragments of functionally active proteins associated with photosynthesis, aquatic transport, and chitin binding. The anti-oomycete effects may be associated with the conversion of ribulose-1,5-bisphosphate carboxylase/oxygenase to produce a number of biologically active anionic peptides with possible regulatory functions. These data inform our knowledge regarding biologically active peptide fragments; they are the components of programmed or induced proteolysis of plant proteins and can realize secondary antimicrobial functions.
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Claims 7
Claim · model-written summary
All seven medicinal-plant extracts inhibited Phytophthora zoospore output.
- Taxon
- medicinal plantst0
- Treatment
- extractse0
- Interactor
- Phytophthora infestans
Phytophthora infestanst0pathogen - Outcome
- zoospores outpute0
- Direction
- inhibitorye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all the extracts possess inhibitory activity toward the zoospores output”
t0“Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease”
Claim · model-written summary
All extracts inhibited Phytophthora zoosporangium germination.
Not reportedComparator
Verbatim
e0“all the extracts possess inhibitory activity toward the zoospores output, zoosporangium germination”
t0“Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease”
Claim · model-written summary
All extracts inhibited Phytophthora development on potato tuber discs to differing degrees.
- Taxon
- potato
Solanum tuberosume0 - Treatment
- extractse0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Outcome
- development of P. infestans on potato disc tuberse0
- Direction
- inhibitorye0
- Qualifier
- different quantitative levelse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all the extracts possess inhibitory activity toward the zoospores output, zoosporangium germination, and the development of P. infestans on potato disc tubers at different quantitative levels”
t0“Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease”
Claim · model-written summary
Common horsetail extract was the most effective among the extracts.
- Taxon
- common horsetail
Equisetum arvensee0 - Treatment
- extract of common horsetail (Equisetum arvense) biomasse0
- Outcome
- most effectivee0
- Direction
- most effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an extract of common horsetail (Equisetum arvense) biomass is recognized as the most effective”
t0“Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease”
Claim · model-written summary
Horsetail extract contained Asn/Asp- and Gln/Glu-rich short peptides mainly of 500–900 Da and no more than 1500 Da.
- Taxon
- common horsetailEquisetum arvensee1
- Outcome
- Asn/Asp- and Gln/Glu-rich short peptidese0
- Value
- 500–900 Dae0
- Qualifier
- not exceeding 1500 Dae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“presence of Asn/Asp- and Gln/Glu-rich short peptides with molecular masses in the range of 500–900 Da and not exceeding 1500 Da as the maximum”
t0“Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease”
e1“common horsetail (Equisetum arvense) biomass”
Claim · model-written summary
Anion-exchange HPLC separated nine dominant horsetail peptide components into two retention-time groups.
- Taxon
- common horsetailEquisetum arvensee0
- Outcome
- nine dominant componentse0
- Value
- ninee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analytical anion-exchange HPLC is successfully applied for separation of the peptide extract from common horsetail (E. arvense). We collect nine dominant components that are combined in two groups with differences in retention times”
t0“Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease”
Claim · model-written summary
The prevalent horsetail peptides were identified as fragments of proteins associated with photosynthesis, aquatic transport and chitin binding.
- Taxon
- common horsetailEquisetum arvensee1
- Outcome
- peptide fragments of functionally active proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identify them as peptide fragments of functionally active proteins associated with photosynthesis, aquatic transport, and chitin binding”
t0“Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease”
e1“common horsetail (Equisetum arvense) biomass”
The same questions in other papers
botanical · Phytophthora infestans · Solanum tuberosum (potato)Positive and negative results
15 papers in the same direction as this paper · 2 in the opposite direction
- positive
- negative
- no effect
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.