Abstract
By EST sequencing we identified 2866 different genes from a cold induced cDNA library from the British winter oat variety Gerald (see other abstract by Bräutigam et al). Among these there are several genes similar to cold and/or drought-induced genes previously identified in rice, wheat, rye, barley and Arabidopsis. More than 100 genes encoded putative transcription factors. Of particular importance for the regulation of cold acclimation is the CBF transcription factor family genes, which have important regulatory roles in the cold signalling pathway. In all CBF proteins analysed so far, a characteristic AP2-binding domain has been found. This interacts with a DRE/CRT regulatory element and activates transcription of downstream cold and dehydration responsive genes. Four CBF factor sequences were found in the oat EST collection, belonging to at least 2 different gene families. A full-length cDNA clone from one such gene, denoted AsCBF1, was isolated and sequenced. This revealed a 909 bp long open reading frame (ORF) encoding a putative protein of 303 aa that contain an AP2-binding domain. By semiquantitative RT-PCR analysis we showed that expression of the AsCBF1 gene was induced already 30 minutes after cold induction at +4oC. By chromosomal walking an additional 457 bp upstream of the AsCBF1 translational start codon was isolated. A TATAA-box was then found as well as several other potential regulatory elements including a Myc-recognition site. These elements will now be further characterised both in vitro and in vivo. CBF genes will also be used as genetic engineering tools to develop more cold tolerant oat.
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Claims 10
Claim · model-written summary
EST sequencing identified 2866 genes in a cold-induced oat cDNA library.
- Taxon
- oat
Avena sativae0 - Material
- Geralde0
- Outcome
- different genese0
- Value
- 2866e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By EST sequencing we identified 2866 different genes from a cold induced cDNA library from the British winter oat variety Gerald”
Claim · model-written summary
Several oat genes resembled previously identified cold or drought induced genes.
- Taxon
- oatAvena sativat0
- Outcome
- genes similar to cold and/or drought-induced genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among these there are several genes similar to cold and/or drought-induced genes previously identified in rice, wheat, rye, barley and Arabidopsis.”
t0“oat”
Claim · model-written summary
More than 100 oat genes encoded putative transcription factors.
- Taxon
- oatAvena sativat0
- Outcome
- genes encoded putative transcription factorse0
- Value
- More than 100e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“More than 100 genes encoded putative transcription factors.”
t0“oat”
Claim · model-written summary
Four CBF sequences occurred in the oat EST collection.
- Taxon
- oatAvena sativae0
- Gene
- CBFe0
- Outcome
- CBF factor sequencese0
- Value
- Foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four CBF factor sequences were found in the oat EST collection, belonging to at least 2 different gene families.”
Claim · model-written summary
The CBF sequences belonged to at least two gene families.
- Taxon
- oatAvena sativae0
- Gene
- CBFe0
- Outcome
- different gene familiese0
- Value
- at least 2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four CBF factor sequences were found in the oat EST collection, belonging to at least 2 different gene families.”
Claim · model-written summary
A full length AsCBF1 cDNA clone was isolated and sequenced.
- Taxon
- oatAvena sativat0
- Gene
- AsCBF1e0
- Outcome
- full-length cDNA clonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A full-length cDNA clone from one such gene, denoted AsCBF1, was isolated and sequenced.”
t0“oat”
Claim · model-written summary
AsCBF1 had a 909 bp ORF encoding a 303 aa putative protein with an AP2 binding domain.
- Taxon
- oatAvena sativat0
- Gene
- AsCBF1e1
- Outcome
- open reading frame (ORF)e0
- Value
- 909 bpe0
- Qualifier
- putative protein of 303 aa that contain an AP2-binding domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This revealed a 909 bp long open reading frame (ORF) encoding a putative protein of 303 aa that contain an AP2-binding domain.”
t0“oat”
e1“denoted AsCBF1, was isolated and sequenced”
Claim · model-written summary
AsCBF1 expression was induced within 30 minutes of cold at +4oC.
- Taxon
- oatAvena sativat0
- Treatment
- cold induction at +4oCe0
- Gene
- AsCBF1e0
- Outcome
- expression of the AsCBF1 genee0
- Direction
- inducede0
- Value
- 30 minutese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of the AsCBF1 gene was induced already 30 minutes after cold induction at +4oC.”
t0“oat”
Claim · model-written summary
Chromosomal walking recovered 457 bp upstream of the AsCBF1 start codon.
- Taxon
- oatAvena sativat0
- Gene
- AsCBF1e0
- Outcome
- upstream of the AsCBF1 translational start codone0
- Value
- 457 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By chromosomal walking an additional 457 bp upstream of the AsCBF1 translational start codon was isolated.”
t0“oat”
Claim · model-written summary
A TATAA box and other potential regulatory elements including a Myc recognition site were found.
- Taxon
- oatAvena sativat0
- Outcome
- TATAA-boxe0
- Qualifier
- potential regulatory elements including a Myc-recognition sitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A TATAA-box was then found as well as several other potential regulatory elements including a Myc-recognition site.”
t0“oat”
The same questions in other papers
cold · molecular: expression · Avena sativa (oats)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.