Paper · 2020 · International Journal of Microbiology

In Vitro and In Vivo Antifungal Activity of Clove (Eugenia caryophyllata) and Pepper (Piper nigrum L.) Essential Oils and Functional Extracts Against Fusarium oxysporum and Aspergillus niger in Tomato (Solanum lycopersicum L.)

Laila Muñoz Castellanos; Nubia Amaya Olivas; Juan Ayala-Soto; et al.

5
claims
9
verbatim quotes
53
citations
2
research areas
✓
Open access · cc-by

Abstract

In this study, hydrodistillation was used to obtain essential oils (EOs) from pepper ( Piper nigrum L.) and clove ( Eugenia caryophyllata ) and co-hydrodistillation (addition of fatty acid ethyl esters as extraction co-solvents) was used to obtain functional extracts (FEs). Antifungal activity of EOs and FEs was evaluated by determination of minimum inhibitory concentration (MIC) against Fusarium oxysporum and Aspergillus niger. The results showed that pepper ( Piper nigrum ) and clove ( Eugenia caryophyllata ) essential oils and their functional extracts are effective in vitro at concentrations from 400 to 500 ppm after 10 days of culturing. The essential oils and functional extracts were used on tomato fruit samples at three different concentrations: 350, 400, and 450 ppm 5 . Clove essential oil reduced the growth of Aspergillus niger from 50% to 70% and Fusarium oxysporum to 40%. The functional extracts (FEs) of clove and pepper, mixed with ethyl decanoate (FEs-C 10 ), were the best combination for protecting the tomato fruit in vivo against both phytopathogenic fungi. Gas chromatography-mass spectrometry (GC-MS) was used to identify eugenol as the principal compound in clove oil and limonene, sabinene, and β -caryophyllene in pepper oil.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Clove essential oil reduced Aspergillus niger growth by 50% to 70%.

Treatment
Clove essential oile2
Interactor
Aspergillus nigerAspergillus nigere2pathogen
Outcome
growthe2
Direction
reducede2
Value
50% to 70%e2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Aspergillus niger
Aspergillus niger · InteractorNo machine-readable author provided. Y tambe assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Tomato (Solanum lycopersicum L.)”

✓ located verbatim in the source · title, characters 194–226 · show in text ↑
e2

“Clove essential oil reduced the growth of Aspergillus niger from 50% to 70% and Fusarium oxysporum to 40%”

✓ located verbatim in the source · abstract, characters 789–894 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Clove essential oil reduced Fusarium oxysporum growth to 40%.

Treatment
Clove essential oile2
Interactor
Fusarium oxysporumFusarium oxysporume2pathogen
Outcome
growthe2
Direction
reducede2
Value
40%e2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Tomato (Solanum lycopersicum L.)”

✓ located verbatim in the source · title, characters 194–226 · show in text ↑
e2

“Clove essential oil reduced the growth of Aspergillus niger from 50% to 70% and Fusarium oxysporum to 40%”

✓ located verbatim in the source · abstract, characters 789–894 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Functional extracts of clove and pepper mixed with ethyl decanoate were the best combination for protecting tomato fruit in vivo against Fusarium oxysporum and Aspergillus niger.

Treatment
functional extracts (FEs) of clove and pepper, mixed with ethyl decanoatee3
Interactor
Fusarium oxysporumFusarium oxysporume1pathogen
Second interactor
Aspergillus nigerAspergillus nigere1pathogen
Outcome
protecting the tomato fruit in vivoe3
Direction
beste3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗
Aspergillus niger
Aspergillus niger · Second interactorNo machine-readable author provided. Y tambe assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Tomato (Solanum lycopersicum L.)”

✓ located verbatim in the source · title, characters 194–226 · show in text ↑
e1

“Antifungal activity of EOs and FEs was evaluated by determination of minimum inhibitory concentration (MIC) against Fusarium oxysporum and Aspergillus niger”

✓ located verbatim in the source · abstract, characters 275–431 · show in text ↑
e3

“The functional extracts (FEs) of clove and pepper, mixed with ethyl decanoate (FEs-C 10 ), were the best combination for protecting the tomato fruit in vivo against both phytopathogenic fungi”

✓ located verbatim in the source · abstract, characters 896–1087 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

GC-MS identified eugenol as the principal compound in clove oil.

Outcome
eugenol as the principal compound in clove oile4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Syzygium aromaticum
Syzygium aromaticum · TaxonBayu Nugroho · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e4

“identify eugenol as the principal compound in clove oil and limonene, sabinene, and β -caryophyllene in pepper oil”

✓ located verbatim in the source · abstract, characters 1146–1260 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

GC-MS identified limonene, sabinene and β-caryophyllene as the principal compounds in pepper oil.

Taxon
peppere4
Outcome
limonene, sabinene, and β -caryophyllene in pepper oile4

Verbatim

e4

“identify eugenol as the principal compound in clove oil and limonene, sabinene, and β -caryophyllene in pepper oil”

✓ located verbatim in the source · abstract, characters 1146–1260 · show in text ↑

botanical · Fusarium oxysporum · Solanum lycopersicum (tomato)Positive and negative results

25 papers: 23 positive, 2 negative or no effect

22 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. no effect
  3. positive

21 papers more in this group

See all 25 papers →

botanical · Aspergillus niger · Solanum lycopersicum (tomato)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.