Abstract
Grape polyphenols contributing to more than half of the global polyphenol market were well studied; however, how melatonin (MLT), a potential plant hormone, and abscisic acid (ABA) affects polyphenols profile is still poorly understood. To explore whether these hormones are involved in polyphenolic biosynthesis, grape (Vitis vinifera cv. Kyoho) was exposed to MLT, ABA, and NDGA (nordihydroguaiaretic acid, an ABA biosynthesis inhibitor) treatments, and 16 polyphenols were identified from grape extracts by high performance liquid chromatography quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS). Both exogenous MLT and ABA significantly enhanced the biosynthesis of each flavonol and flavanol component, especially catechin, which was almost increased double by 200 µM of MLT treatment. Furthermore, the expression of genes involved in flavonoid biosynthesis, including 4-coumaroyl-CoA synthase, chalcone synthase, flavonoid 3′-hydroxylase, anthocyanin 3′-methyltransferase, flavonol synthase, flavonoid-3-O-glucosyltransferase, and flavonoid 3′,5′-methyltransferase were highly up-regulated as well but were down-regulated by NDGA. The present study provided new insights for improving flavonoids accumulation in agricultural production and its underlying mechanism.
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Claims 6
Claim · model-written summary
Sixteen polyphenols were identified in grape extracts.
Verbatim
e0“16 polyphenols were identified from grape extracts by high performance liquid chromatography quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS).”
Claim · model-written summary
Exogenous melatonin significantly enhanced synthesis of flavonol and flavanol components.
- Taxon
- grapet0
- Treatment
- exogenous MLTe0
- Outcome
- biosynthesis of each flavonol and flavanol componente0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“Both exogenous MLT and ABA significantly enhanced the biosynthesis of each flavonol and flavanol component”
t0“Grape Berry”
Claim · model-written summary
Exogenous ABA significantly enhanced synthesis of flavonol and flavanol components.
- Taxon
- grapet0
- Treatment
- ABAe0
- Outcome
- biosynthesis of each flavonol and flavanol componente0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“Both exogenous MLT and ABA significantly enhanced the biosynthesis of each flavonol and flavanol component”
t0“Grape Berry”
Claim · model-written summary
A 200 µM melatonin treatment almost doubled catechin.
- Taxon
- grapet0
- Treatment
- 200 µM of MLT treatmente0
- Outcome
- catechine0
- Direction
- increasede0
- Value
- almost increased doublee0
Not reportedComparator
Verbatim
e0“especially catechin, which was almost increased double by 200 µM of MLT treatment.”
t0“Grape Berry”
Claim · model-written summary
MLT and ABA upregulated expression of the listed flavonoid biosynthesis genes.
- Taxon
- grapet0
- Treatment
- exogenous MLT and ABAe0
- Gene
- 4-coumaroyl-CoA synthase, chalcone synthase, flavonoid 3′-hydroxylasee0
- Outcome
- expression of genes involved in flavonoid biosynthesise0
- Direction
- up-regulatede0
Not reportedComparator
Verbatim
e0“Both exogenous MLT and ABA significantly enhanced the biosynthesis of each flavonol and flavanol component, especially catechin, which was almost increased double by 200 µM of MLT treatment. Furthermore, the expression of genes involved in flavonoid biosynthesis, including 4-coumaroyl-CoA synthase, chalcone synthase, flavonoid 3′-hydroxylase, anthocyanin 3′-methyltransferase, flavonol synthase, flavonoid-3-O-glucosyltransferase, and flavonoid 3′,5′-methyltransferase were highly up-regulated as well”
t0“Grape Berry”
Claim · model-written summary
NDGA downregulated expression of flavonoid biosynthesis genes.
- Taxon
- grapet0
- Treatment
- NDGAe0
- Gene
- 4-coumaroyl-CoA synthase, chalcone synthase, flavonoid 3′-hydroxylasee0
- Outcome
- expression of genes involved in flavonoid biosynthesise0
- Direction
- down-regulatede0
Not reportedComparator
Verbatim
e0“the expression of genes involved in flavonoid biosynthesis, including 4-coumaroyl-CoA synthase, chalcone synthase, flavonoid 3′-hydroxylase, anthocyanin 3′-methyltransferase, flavonol synthase, flavonoid-3-O-glucosyltransferase, and flavonoid 3′,5′-methyltransferase were highly up-regulated as well but were down-regulated by NDGA.”
t0“Grape Berry”
The same questions in other papers
(+)-abscisic acid · molecular: metabolites · VitisPositive and negative results
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- no effect
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.