Abstract
Fresh fruits and vegetables (FFVs) are the most frequently wasted foods because of their perishability and handling requirements. However, there is a lack of information on how much each step of the supply chain impacts FFVs quality, particularly on tomatoes, and what measures need to be taken for an immediate and effective impact on waste reduction. There is also no information on how the supply chain affects the proteome of the tomato and what proteins are differentially regulated by the most impactful steps of the supply chain. The objectives of the work presented on this thesis were to evaluate the decline in the overall quality and quantify tomato waste at each step of the supply chain, from the farm to consumer; and to determine what proteins are impacted by the decline in quality that is associated with temperature abuse. To determine overall quality and tomato waste, light-red tomatoes were exposed to an optimum temperature (13 °C) and eighteen different time-temperature scenarios, normally encountered during supply chain, and sensory and physicochemical attributes measured at each step. To determine the impact of chilling and non-chilling temperatures normally encountered during tomato supply chain, on the proteome, light-red tomatoes were exposed to an optimum temperature (13 °C) and to two time and temperature supply chain scenarios (2 °C and 25 °C) that showed the most negative impact on tomato overall quality, and physicochemical and proteomic attributes were measured at each step. For the first tomato harvest, the steps with the highest impact on quality and waste were shipping to distribution center (DC; 20°C), cooling at the grower (25°C) and storing at the consumer (4°C). For the second tomato harvest, shipping to the store (2°C), cooling at the farm (10°C) and displaying at the store (20°C) negatively impacted quality. High temperatures during cooling, shipping and store display impacted sensory quality and resulted in increased weight loss, and decreased sugar, carotenoids, and ascorbic acid contents. Although low temperatures during shipping, cooling and consumer did not impact tomato sensory quality, they contributed to a decline in sugar, carotenoids and ascorbic acid contents. Overall, the most impactful steps on tomato quality and waste, regardless of the temperature, were shipping to DC, cooling, shipping to stores, displaying at the store, and consumer storage. Analysis of the differentially expressed proteins in the tomato showed that metabolic proteins were greatly impacted by temperature abuses such as phosphomannomutase, heme oxygenase 1, and MAP kinase; and that proteins regulating cellular membrane integrity such as vacuolar protein sorting-associated protein were also impacted.
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Claims 10
Claim · model-written summary
For the first harvest, shipping to the distribution center, grower cooling and consumer storage most affected tomato quality and waste.
- Taxon
- tomato
Solanum lycopersicume0 - Outcome
- quality and wastee0
- Setting
- shipping to distribution center (DC; 20°C), cooling at the grower (25°C) and storing at the consumer (4°C)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“For the first tomato harvest, the steps with the highest impact on quality and waste were shipping to distribution center (DC; 20°C), cooling at the grower (25°C) and storing at the consumer (4°C).”
Claim · model-written summary
For the second harvest, shipping to store, farm cooling and store display negatively affected tomato quality.
- Taxon
- tomato
Solanum lycopersicume0 - Outcome
- qualitye0
- Setting
- shipping to the store (2°C), cooling at the farm (10°C) and displaying at the store (20°C)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“For the second tomato harvest, shipping to the store (2°C), cooling at the farm (10°C) and displaying at the store (20°C) negatively impacted quality.”
Claim · model-written summary
High temperatures during cooling, shipping and display degraded tomato sensory quality.
- Taxon
- Tomato
Solanum lycopersicumt0 - Treatment
- High temperaturese0
- Outcome
- sensory qualitye0
- Setting
- cooling, shipping and store displaye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“High temperatures during cooling, shipping and store display impacted sensory quality and resulted in increased weight loss, and decreased sugar, carotenoids, and ascorbic acid contents.”
t0“Tomato Fruit Quality”
Claim · model-written summary
High temperatures increased tomato weight loss.
- Taxon
- Tomato
Solanum lycopersicumt0 - Treatment
- High temperaturese0
- Outcome
- weight losse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“High temperatures during cooling, shipping and store display impacted sensory quality and resulted in increased weight loss, and decreased sugar, carotenoids, and ascorbic acid contents.”
t0“Tomato Fruit Quality”
Claim · model-written summary
High temperatures decreased tomato sugar, carotenoid and ascorbic acid contents.
- Taxon
- Tomato
Solanum lycopersicumt0 - Treatment
- High temperaturese0
- Outcome
- sugar, carotenoids, and ascorbic acid contentse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“High temperatures during cooling, shipping and store display impacted sensory quality and resulted in increased weight loss, and decreased sugar, carotenoids, and ascorbic acid contents.”
t0“Tomato Fruit Quality”
Claim · model-written summary
Low temperatures did not affect tomato sensory quality.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- low temperaturese0
- Outcome
- sensory qualitye0
- Direction
- did not impacte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“low temperatures during shipping, cooling and consumer did not impact tomato sensory quality”
Claim · model-written summary
Low temperatures reduced tomato sugar, carotenoid and ascorbic acid contents.
- Taxon
- Tomato
Solanum lycopersicumt0 - Treatment
- low temperaturese0
- Outcome
- sugar, carotenoids and ascorbic acid contentse0
- Direction
- declinee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“low temperatures during shipping, cooling and consumer did not impact tomato sensory quality, they contributed to a decline in sugar, carotenoids and ascorbic acid contents.”
t0“Tomato Fruit Quality”
Claim · model-written summary
Shipping, cooling, store display and consumer storage were the most impactful steps for tomato quality and waste.
- Taxon
- tomato
Solanum lycopersicume0 - Outcome
- quality and wastee0
- Setting
- shipping to DC, cooling, shipping to stores, displaying at the store, and consumer storagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the most impactful steps on tomato quality and waste, regardless of the temperature, were shipping to DC, cooling, shipping to stores, displaying at the store, and consumer storage.”
Claim · model-written summary
Temperature abuse affected tomato metabolic proteins.
- Taxon
- Tomato
Solanum lycopersicumt0 - Treatment
- temperature abusese0
- Gene
- phosphomannomutase, heme oxygenase 1, and MAP kinasee0
- Outcome
- metabolic proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“metabolic proteins were greatly impacted by temperature abuses such as phosphomannomutase, heme oxygenase 1, and MAP kinase”
t0“Tomato Fruit Quality”
Claim · model-written summary
Temperature abuse also affected proteins regulating tomato cellular membrane integrity.
- Taxon
- Tomato
Solanum lycopersicumt0 - Gene
- vacuolar protein sorting-associated proteine0
- Outcome
- proteins regulating cellular membrane integritye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“proteins regulating cellular membrane integrity such as vacuolar protein sorting-associated protein were also impacted.”
t0“Tomato Fruit Quality”
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