Paper · 2019 · Digital Commons - University of South Florida (University of South Florida)

Biochemical and Proteomic Approaches to Determine the Impact Level of Each Step of the Supply Chain on Tomato Fruit Quality

Robert Madden

10
claims
16
verbatim quotes
0
citations
3
research areas
✓
Open access

Abstract

Fresh fruits and vegetables (FFVs) are the most frequently wasted foods because of their perishability and handling requirements. However, there is a lack of information on how much each step of the supply chain impacts FFVs quality, particularly on tomatoes, and what measures need to be taken for an immediate and effective impact on waste reduction. There is also no information on how the supply chain affects the proteome of the tomato and what proteins are differentially regulated by the most impactful steps of the supply chain. The objectives of the work presented on this thesis were to evaluate the decline in the overall quality and quantify tomato waste at each step of the supply chain, from the farm to consumer; and to determine what proteins are impacted by the decline in quality that is associated with temperature abuse. To determine overall quality and tomato waste, light-red tomatoes were exposed to an optimum temperature (13 °C) and eighteen different time-temperature scenarios, normally encountered during supply chain, and sensory and physicochemical attributes measured at each step. To determine the impact of chilling and non-chilling temperatures normally encountered during tomato supply chain, on the proteome, light-red tomatoes were exposed to an optimum temperature (13 °C) and to two time and temperature supply chain scenarios (2 °C and 25 °C) that showed the most negative impact on tomato overall quality, and physicochemical and proteomic attributes were measured at each step. For the first tomato harvest, the steps with the highest impact on quality and waste were shipping to distribution center (DC; 20°C), cooling at the grower (25°C) and storing at the consumer (4°C). For the second tomato harvest, shipping to the store (2°C), cooling at the farm (10°C) and displaying at the store (20°C) negatively impacted quality. High temperatures during cooling, shipping and store display impacted sensory quality and resulted in increased weight loss, and decreased sugar, carotenoids, and ascorbic acid contents. Although low temperatures during shipping, cooling and consumer did not impact tomato sensory quality, they contributed to a decline in sugar, carotenoids and ascorbic acid contents. Overall, the most impactful steps on tomato quality and waste, regardless of the temperature, were shipping to DC, cooling, shipping to stores, displaying at the store, and consumer storage. Analysis of the differentially expressed proteins in the tomato showed that metabolic proteins were greatly impacted by temperature abuses such as phosphomannomutase, heme oxygenase 1, and MAP kinase; and that proteins regulating cellular membrane integrity such as vacuolar protein sorting-associated protein were also impacted.

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Claims 10

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

For the first harvest, shipping to the distribution center, grower cooling and consumer storage most affected tomato quality and waste.

Outcome
quality and wastee0
Setting
shipping to distribution center (DC; 20°C), cooling at the grower (25°C) and storing at the consumer (4°C)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“For the first tomato harvest, the steps with the highest impact on quality and waste were shipping to distribution center (DC; 20°C), cooling at the grower (25°C) and storing at the consumer (4°C).”

✓ located verbatim in the source · abstract, characters 1520–1717 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

For the second harvest, shipping to store, farm cooling and store display negatively affected tomato quality.

Outcome
qualitye0
Setting
shipping to the store (2°C), cooling at the farm (10°C) and displaying at the store (20°C)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“For the second tomato harvest, shipping to the store (2°C), cooling at the farm (10°C) and displaying at the store (20°C) negatively impacted quality.”

✓ located verbatim in the source · abstract, characters 1718–1868 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

High temperatures during cooling, shipping and display degraded tomato sensory quality.

Treatment
High temperaturese0
Outcome
sensory qualitye0
Setting
cooling, shipping and store displaye0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“High temperatures during cooling, shipping and store display impacted sensory quality and resulted in increased weight loss, and decreased sugar, carotenoids, and ascorbic acid contents.”

✓ located verbatim in the source · abstract, characters 1869–2055 · show in text ↑
t0

“Tomato Fruit Quality”

✓ located verbatim in the source · title, characters 103–123 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

High temperatures increased tomato weight loss.

Treatment
High temperaturese0
Outcome
weight losse0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“High temperatures during cooling, shipping and store display impacted sensory quality and resulted in increased weight loss, and decreased sugar, carotenoids, and ascorbic acid contents.”

✓ located verbatim in the source · abstract, characters 1869–2055 · show in text ↑
t0

“Tomato Fruit Quality”

✓ located verbatim in the source · title, characters 103–123 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

High temperatures decreased tomato sugar, carotenoid and ascorbic acid contents.

Treatment
High temperaturese0
Outcome
sugar, carotenoids, and ascorbic acid contentse0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“High temperatures during cooling, shipping and store display impacted sensory quality and resulted in increased weight loss, and decreased sugar, carotenoids, and ascorbic acid contents.”

✓ located verbatim in the source · abstract, characters 1869–2055 · show in text ↑
t0

“Tomato Fruit Quality”

✓ located verbatim in the source · title, characters 103–123 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Low temperatures did not affect tomato sensory quality.

Treatment
low temperaturese0
Outcome
sensory qualitye0
Direction
did not impacte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“low temperatures during shipping, cooling and consumer did not impact tomato sensory quality”

✓ located verbatim in the source · abstract, characters 2065–2157 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Low temperatures reduced tomato sugar, carotenoid and ascorbic acid contents.

Treatment
low temperaturese0
Outcome
sugar, carotenoids and ascorbic acid contentse0
Direction
declinee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“low temperatures during shipping, cooling and consumer did not impact tomato sensory quality, they contributed to a decline in sugar, carotenoids and ascorbic acid contents.”

✓ located verbatim in the source · abstract, characters 2065–2238 · show in text ↑
t0

“Tomato Fruit Quality”

✓ located verbatim in the source · title, characters 103–123 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Shipping, cooling, store display and consumer storage were the most impactful steps for tomato quality and waste.

Outcome
quality and wastee0
Setting
shipping to DC, cooling, shipping to stores, displaying at the store, and consumer storagee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the most impactful steps on tomato quality and waste, regardless of the temperature, were shipping to DC, cooling, shipping to stores, displaying at the store, and consumer storage.”

✓ located verbatim in the source · abstract, characters 2248–2429 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Temperature abuse affected tomato metabolic proteins.

Treatment
temperature abusese0
Gene
phosphomannomutase, heme oxygenase 1, and MAP kinasee0
Outcome
metabolic proteinse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“metabolic proteins were greatly impacted by temperature abuses such as phosphomannomutase, heme oxygenase 1, and MAP kinase”

✓ located verbatim in the source · abstract, characters 2506–2629 · show in text ↑
t0

“Tomato Fruit Quality”

✓ located verbatim in the source · title, characters 103–123 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Temperature abuse also affected proteins regulating tomato cellular membrane integrity.

Gene
vacuolar protein sorting-associated proteine0
Outcome
proteins regulating cellular membrane integritye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“proteins regulating cellular membrane integrity such as vacuolar protein sorting-associated protein were also impacted.”

✓ located verbatim in the source · abstract, characters 2640–2759 · show in text ↑
t0

“Tomato Fruit Quality”

✓ located verbatim in the source · title, characters 103–123 · show in text ↑

cold · physiology: pigments · Solanum lycopersicum (tomato)Results differ

13 papers: 5 positive, 8 negative or no effect

7 papers in the same direction as this paper · 5 in the opposite direction

  1. positive
  2. positive
  3. positive

9 papers more in this group

See all 13 papers →

heat · physiology: pigments · Solanum lycopersicum (tomato)Results differ

20 papers: 8 positive, 12 negative or no effect

11 papers in the same direction as this paper · 8 in the opposite direction

  1. positive
  2. positive
  3. positive

16 papers more in this group

See all 20 papers →

heat · quality: postharvest · Solanum lycopersicum (tomato)Results differ

36 papers: 29 positive, 7 negative or no effect

6 papers in the same direction as this paper · 29 in the opposite direction

  1. positive
  2. positive
  3. positive

32 papers more in this group

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heat · quality: general · Solanum lycopersicum (tomato)Positive and negative results

11 papers: 6 positive, 5 negative or no effect

4 papers in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. positive
  3. positive

7 papers more in this group

See all 11 papers →

cold · quality: general · Solanum lycopersicum (tomato)Positive and negative results

15 papers: 9 positive, 6 negative or no effect

5 papers in the same direction as this paper · 9 in the opposite direction

  1. negative
  2. positive
  3. positive

11 papers more in this group

See all 15 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.