Abstract
Although the alteration of DNA methylation due to abiotic stresses, such as exposure to the toxic metal cadmium (Cd), has been often observed in plants, little is known about whether such epigenetic changes are linked to the ability of plants to adapt to stress. Herein, we report a close linkage between DNA methylation and the adaptational responses in Arabidopsis plants under Cd stress. Exposure to Cd significantly inhibited the expression of three DNA demethylase genes ROS1/DML2/DML3 (RDD) and elevated DNA methylation at the genome-wide level in Col-0 roots. Furthermore, the profile of DNA methylation in Cd-exposed Col-0 roots was similar to that in the roots of rdd triple mutants, which lack RDD, indicating that Cd-induced DNA methylation is associated with the inhibition of RDD. Interestingly, the elevation in DNA methylation in rdd conferred a higher tolerance against Cd stress and improved cellular Fe nutrition in the root tissues. In addition, lowering the Fe supply abolished improved Cd tolerance due to the lack of RDD in rdd. Together, these data suggest that the inhibition of RDD-mediated DNA demethylation in the roots by Cd would in turn enhance plant tolerance to Cd stress by improving Fe nutrition through a feedback mechanism.
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Claims 7
Claim · model-written summary
Cadmium exposure inhibited expression of ROS1/DML2/DML3 in Col-0 roots.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- Cde0
- Gene
- ROS1/DML2/DML3 (RDD)e0
- Outcome
- expression of three DNA demethylase genes ROS1/DML2/DML3 (RDD)e0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure to Cd significantly inhibited the expression of three DNA demethylase genes ROS1/DML2/DML3 (RDD)”
e1“Arabidopsis plants”
Claim · model-written summary
Cadmium elevated genome-wide DNA methylation in Col-0 roots.
- Taxon
- ArabidopsisArabidopsise2
- Material
- Col-0e0
- Organ / stage
- rootse0
- Treatment
- Cde1
- Outcome
- DNA methylation at the genome-wide levele0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elevated DNA methylation at the genome-wide level in Col-0 roots”
e1“Exposure to Cd”
e2“Arabidopsis plants”
Claim · model-written summary
The methylation profile of cadmium-exposed Col-0 roots resembled that of rdd triple-mutant roots.
- Taxon
- ArabidopsisArabidopsise1
- Material
- Col-0e0
- Outcome
- profile of DNA methylatione0
- Direction
- similare0
- Comparator
- rdd triple mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the profile of DNA methylation in Cd-exposed Col-0 roots was similar to that in the roots of rdd triple mutants”
e1“Arabidopsis plants”
Claim · model-written summary
Elevated methylation in rdd increased cadmium tolerance.
- Taxon
- ArabidopsisArabidopsise1
- Material
- rdde0
- Outcome
- tolerance against Cd stresse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the elevation in DNA methylation in rdd conferred a higher tolerance against Cd stress”
e1“Arabidopsis plants”
Claim · model-written summary
Elevated methylation in rdd improved cellular iron nutrition in roots.
- Taxon
- ArabidopsisArabidopsise1
- Material
- rdde0
- Organ / stage
- root tissuese0
- Outcome
- cellular Fe nutritione0
- Direction
- improvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the elevation in DNA methylation in rdd conferred a higher tolerance against Cd stress and improved cellular Fe nutrition in the root tissues”
e1“Arabidopsis plants”
Claim · model-written summary
Lower iron supply abolished the improved cadmium tolerance of rdd.
- Taxon
- ArabidopsisArabidopsise1
- Material
- rdde0
- Treatment
- lowering the Fe supplye0
- Outcome
- improved Cd tolerancee0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lowering the Fe supply abolished improved Cd tolerance due to the lack of RDD in rdd”
e1“Arabidopsis plants”
Claim · model-written summary
Authors infer that cadmium inhibition of RDD-mediated demethylation enhances cadmium tolerance by improving iron nutrition.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- RDDe0
- Outcome
- plant tolerance to Cd stresse0
- Direction
- enhancee0
- Qualifier
- by improving Fe nutrition through a feedback mechanisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the inhibition of RDD-mediated DNA demethylation in the roots by Cd would in turn enhance plant tolerance to Cd stress by improving Fe nutrition through a feedback mechanism”
e1“Arabidopsis plants”
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.