Abstract
Reactive oxygen species play a central role in the regulation of plant responses to environmental stress. Under prolonged iron (Fe) deficiency, increased levels of hydrogen peroxide (H2O2) initiate signaling events, resulting in the attenuation of Fe acquisition through the inhibition of FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT). As this H2O2 increase occurs in a FIT-dependent manner, our aim was to understand the processes involved in maintaining H2O2 levels under prolonged Fe deficiency and the role of FIT. We identified the CAT2 gene, encoding one of the three Arabidopsis catalase isoforms, as regulated by FIT. CAT2 loss-of-function plants displayed severe susceptibility to Fe deficiency and greatly increased H2O2 levels in roots. Analysis of the Fe homeostasis transcription cascade revealed that H2O2 influences the gene expression of downstream regulators FIT, BHLH genes of group Ib, and POPEYE (PYE); however, H2O2 did not affect their upstream regulators, such as BHLH104 and ILR3. Our data shows that FIT and CAT2 participate in a regulatory loop between H2O2 and prolonged Fe deficiency.
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Claims 6
Claim · model-written summary
The CAT2 gene, encoding an Arabidopsis catalase isoform, is regulated by FIT.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- CAT2e0
- Outcome
- regulated by FITe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified the CAT2 gene, encoding one of the three Arabidopsis catalase isoforms, as regulated by FIT”
Claim · model-written summary
CAT2 loss-of-function plants showed severe susceptibility to Fe deficiency.
- Taxon
- ArabidopsisArabidopsise0
- Material
- CAT2 loss-of-function plantse1
- Treatment
- Fe deficiencye1
- Gene
- CAT2e1
- Outcome
- severe susceptibilitye1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified the CAT2 gene, encoding one of the three Arabidopsis catalase isoforms, as regulated by FIT”
e1“CAT2 loss-of-function plants displayed severe susceptibility to Fe deficiency and greatly increased H2O2 levels in roots”
Claim · model-written summary
CAT2 loss-of-function plants had greatly increased H2O2 levels in roots under Fe deficiency.
- Taxon
- ArabidopsisArabidopsise0
- Material
- CAT2 loss-of-function plantse1
- Treatment
- Fe deficiencye1
- Outcome
- H2O2 levels in rootse1
- Direction
- increasede1
- Qualifier
- greatlye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified the CAT2 gene, encoding one of the three Arabidopsis catalase isoforms, as regulated by FIT”
e1“CAT2 loss-of-function plants displayed severe susceptibility to Fe deficiency and greatly increased H2O2 levels in roots”
Claim · model-written summary
H2O2 influences the gene expression of the downstream regulators FIT, BHLH genes of group Ib and PYE.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- H2O2e2
- Gene
- FIT, BHLH genes of group Ib, and POPEYE (PYE)e2
- Outcome
- gene expression of downstream regulators FIT, BHLH genes of group Ib, and POPEYE (PYE)e2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis”
e2“H2O2 influences the gene expression of downstream regulators FIT, BHLH genes of group Ib, and POPEYE (PYE)”
Claim · model-written summary
H2O2 did not affect the upstream regulators BHLH104 and ILR3.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- H2O2e3
- Gene
- BHLH104 and ILR3e3
- Outcome
- upstream regulators, such as BHLH104 and ILR3e3
- Direction
- did not affecte3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis”
e3“however, H2O2 did not affect their upstream regulators, such as BHLH104 and ILR3”
Claim · model-written summary
FIT and CAT2 participate in a regulatory loop between H2O2 and prolonged Fe deficiency.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- FIT and CAT2e4
- Outcome
- regulatory loop between H2O2 and prolonged Fe deficiencye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis”
e4“Our data shows that FIT and CAT2 participate in a regulatory loop between H2O2 and prolonged Fe deficiency”
The same questions in other papers
iron atom · disease · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.