Abstract
With 1 AP2 domain and 1 B3 domain, 7 MeRAVs in apetala2/ethylene response factor (AP2/ERF) gene family have been identified in cassava. However, the in vivo roles of these remain unknown. Gene expression assays showed that the transcripts of MeRAVs were commonly regulated after Xanthomonas axonopodis pv manihotis (Xam) and MeRAVs were specifically located in plant cell nuclei. Through virus-induced gene silencing (VIGS) in cassava, we found that MeRAV1 and MeRAV2 are essential for plant disease resistance against cassava bacterial blight, as shown by the bacterial propagation of Xam in plant leaves. Through VIGS in cassava leaves and overexpression in cassava leave protoplasts, we found that MeRAV1 and MeRAV2 positively regulated melatonin biosynthesis genes and the endogenous melatonin level. Further investigation showed that MeRAV1 and MeRAV2 are direct transcriptional activators of 3 melatonin biosynthesis genes in cassava, as evidenced by chromatin immunoprecipitation-PCR in cassava leaf protoplasts and electrophoretic mobility shift assay. Moreover, cassava melatonin biosynthesis genes also positively regulated plant disease resistance. Taken together, this study identified MeRAV1 and MeRAV2 as common and upstream transcription factors of melatonin synthesis genes in cassava and revealed a model of MeRAV1 and MeRAV2-melatonin biosynthesis genes-melatonin level in plant disease resistance against cassava bacterial blight.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Seven MeRAV transcription factors were identified in cassava.
- Taxon
- cassava
Manihot esculentae0 - Gene
- MeRAVse0
- Outcome
- 7 MeRAVse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7 MeRAVs in apetala2/ethylene response factor (AP2/ERF) gene family have been identified in cassava”
Claim · model-written summary
MeRAV transcripts were regulated after Xam exposure.
- Taxon
- cassavaManihot esculentat0
- Treatment
- Xanthomonas axonopodis pv manihotis (Xam)e0
- Interactor
- Xanthomonas axonopodis pv manihotis
Xanthomonas axonopodise0pathogen - Gene
- MeRAVse0
- Outcome
- transcripts of MeRAVse0
- Direction
- regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcripts of MeRAVs were commonly regulated after Xanthomonas axonopodis pv manihotis (Xam)”
t0“cassava”
Claim · model-written summary
MeRAVs were specifically located in plant cell nuclei.
- Taxon
- cassavaManihot esculentat0
- Gene
- MeRAVse0
- Outcome
- plant cell nucleie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeRAVs were specifically located in plant cell nuclei”
t0“cassava”
Claim · model-written summary
Silencing MeRAV1 increased bacterial propagation and showed its necessity for resistance to cassava bacterial blight.
- Taxon
- cassavaManihot esculentae0
- Treatment
- virus-induced gene silencing (VIGS)e0
- Interactor
- Xame0pathogen
- Gene
- MeRAV1e0
- Outcome
- plant disease resistance against cassava bacterial blighte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through virus-induced gene silencing (VIGS) in cassava, we found that MeRAV1 and MeRAV2 are essential for plant disease resistance against cassava bacterial blight, as shown by the bacterial propagation of Xam in plant leaves”
Claim · model-written summary
Silencing MeRAV2 showed its necessity for cassava bacterial blight resistance.
- Taxon
- cassavaManihot esculentae0
- Treatment
- virus-induced gene silencing (VIGS)e0
- Interactor
- Xame0pathogen
- Gene
- MeRAV2e0
- Outcome
- plant disease resistance against cassava bacterial blighte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through virus-induced gene silencing (VIGS) in cassava, we found that MeRAV1 and MeRAV2 are essential for plant disease resistance against cassava bacterial blight, as shown by the bacterial propagation of Xam in plant leaves”
Claim · model-written summary
MeRAV1 positively regulated melatonin biosynthesis genes and endogenous melatonin level.
- Taxon
- cassavaManihot esculentat0
- Gene
- MeRAV1e0
- Outcome
- melatonin biosynthesis genes and the endogenous melatonin levele0
- Direction
- positively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeRAV1 and MeRAV2 positively regulated melatonin biosynthesis genes and the endogenous melatonin level”
t0“cassava”
Claim · model-written summary
MeRAV2 positively regulated melatonin biosynthesis genes and endogenous melatonin level.
- Taxon
- cassavaManihot esculentat0
- Gene
- MeRAV2e0
- Outcome
- melatonin biosynthesis genes and the endogenous melatonin levele0
- Direction
- positively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeRAV1 and MeRAV2 positively regulated melatonin biosynthesis genes and the endogenous melatonin level”
t0“cassava”
Claim · model-written summary
MeRAV1 directly activated three cassava melatonin biosynthesis genes.
- Taxon
- cassavaManihot esculentae0
- Gene
- MeRAV1e0
- Outcome
- 3 melatonin biosynthesis genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeRAV1 and MeRAV2 are direct transcriptional activators of 3 melatonin biosynthesis genes in cassava”
Claim · model-written summary
MeRAV2 directly activated three cassava melatonin biosynthesis genes.
- Taxon
- cassavaManihot esculentae0
- Gene
- MeRAV2e0
- Outcome
- 3 melatonin biosynthesis genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeRAV1 and MeRAV2 are direct transcriptional activators of 3 melatonin biosynthesis genes in cassava”
Claim · model-written summary
Cassava melatonin biosynthesis genes positively regulated disease resistance.
- Taxon
- cassavaManihot esculentae0
- Gene
- melatonin biosynthesis genese0
- Outcome
- plant disease resistancee0
- Direction
- positively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cassava melatonin biosynthesis genes also positively regulated plant disease resistance”
The same questions in other papers
loss of function · disease · Manihot esculenta (cassava)Positive and negative results
4 papers in the same direction as this paper · 6 in the opposite direction
- negative
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.