Abstract
Cassava is a major food crop in tropical areas, but its productivity and quality are seriously limited by cassava bacterial blight. So far, the key factors regulating cassava immune response remain elusive. In this study, we identified three cassava Whirly genes (MeWHYs) in cassava variety of South China 124 (SC124), and explored the possible roles and utilization of MeWHYs in cassava disease resistance. Gene expression analysis revealed that the transcripts of three MeWHYs were commonly regulated by the highly conserved N-terminal epitope of f lagellin (flg22) and Xanthomonas axonopodis pv. manihotis Hainan (Xam HN) treatments. Overexpression of MeWHYs improved plant disease resistance against X. axonopodis pv. manihotis, while MeWHYs-silenced cassava plants by virus-induced gene silencing exhibited decreased disease resistance. Notably, MeWRKY75 physically interacted with three MeWHYs in yeast and in planta, and served as a transcriptional activator of MeWHY3. Moreover, the physical interaction between MeWHYs and MeWRKY75 promoted the transcriptional activities of each other. Consistently, MeWRKY75 also positively regulated disease resistance against cassava bacterial blight. Taken together, our observations suggested that MeWRKY75 and MeWHYs confer improved disease resistance against cassava bacterial blight through forming an interacting complex of MeWRKY75-MeWHY1/2/3 and transcriptional module of MeWRKY75-MeWHY3. This study facilitates our understanding of the positive effect of the MeWRKY75-MeWHY3 transcriptional module in plant disease resistance.
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Claims 7
Claim · model-written summary
Overexpression of MeWHYs improved cassava resistance to X. axonopodis pv. manihotis.
- Taxon
- cassava
Manihot esculentat0 - Treatment
- Overexpression of MeWHYse1
- Interactor
- X. axonopodis pv. manihotisXanthomonas phaseoli pv. manihotise1pathogen
- Gene
- MeWHYse1
- Outcome
- plant disease resistancee1
- Direction
- improvede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Overexpression of MeWHYs improved plant disease resistance against X. axonopodis pv. manihotis”
t0“in plant response to cassava bacterial blight”
Claim · model-written summary
Cassava plants with virus-induced silencing of MeWHYs showed decreased disease resistance.
- Taxon
- cassavaManihot esculentae1
- Treatment
- virus-induced gene silencinge1
- Gene
- MeWHYse1
- Outcome
- disease resistancee1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“MeWHYs-silenced cassava plants by virus-induced gene silencing exhibited decreased disease resistance”
Claim · model-written summary
MeWRKY75 physically interacted with three MeWHYs in yeast and in planta.
- Taxon
- cassavaManihot esculentat0
- Gene
- MeWRKY75e1
- Outcome
- physically interactede1
- Qualifier
- in yeast and in plantae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“MeWRKY75 physically interacted with three MeWHYs in yeast and in planta”
t0“in plant response to cassava bacterial blight”
Claim · model-written summary
MeWRKY75 served as a transcriptional activator of MeWHY3 in cassava.
- Taxon
- cassavaManihot esculentat0
- Gene
- MeWHY3e1
- Outcome
- transcriptional activator of MeWHY3e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“served as a transcriptional activator of MeWHY3”
t0“in plant response to cassava bacterial blight”
Claim · model-written summary
The physical interaction between MeWHYs and MeWRKY75 promoted the transcriptional activities of each other.
- Taxon
- cassavaManihot esculentat0
- Gene
- MeWHYse1
- Outcome
- transcriptional activitiese1
- Direction
- promotede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the physical interaction between MeWHYs and MeWRKY75 promoted the transcriptional activities of each other”
t0“in plant response to cassava bacterial blight”
Claim · model-written summary
MeWRKY75 positively regulated cassava disease resistance against bacterial blight.
- Taxon
- cassavaManihot esculentae1
- Gene
- MeWRKY75e1
- Outcome
- disease resistancee1
- Direction
- positively regulatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“MeWRKY75 also positively regulated disease resistance against cassava bacterial blight”
Claim · model-written summary
Transcripts of three MeWHYs were commonly regulated in cassava by flg22 and by Xanthomonas axonopodis pv. manihotis Hainan.
- Taxon
- cassavaManihot esculentat0
- Treatment
- flg22e1
- Interactor
- Xanthomonas axonopodis pv. manihotis HainanXanthomonas phaseoli pv. manihotise1pathogen
- Gene
- MeWHYse1
- Outcome
- transcripts of three MeWHYse1
- Direction
- commonly regulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the transcripts of three MeWHYs were commonly regulated by the highly conserved N-terminal epitope of f lagellin (flg22) and Xanthomonas axonopodis pv. manihotis Hainan (Xam HN) treatments”
t0“in plant response to cassava bacterial blight”
The same questions in other papers
loss of function · disease · Manihot esculenta (cassava)Positive and negative results
4 papers in the same direction as this paper · 6 in the opposite direction
- positive
- negative
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.