Abstract
The hypocotyl of Chinese kale 'Cutiaoyusun' was used as explants, the effects of the different concentrations of sucrose and different browning inhibitors (activated carbon, polyvinylpyrrolidone, and ascorbic acid) on the callus proliferation and anti-browning of Chinese kale were studied in this study. The results showed that the proliferation medium with 20 g·L−1 sucrose had the best effects on the proliferation and anti-browning of Chinese kale callus, and its proliferation rate was the highest, reaching 213.5%, and the browning rate was as low as 27.77%. In addition, adding 0.2 g·L−1 ascorbic acid to the culture medium can significantly promote callus proliferation and reduce browning rate. In this treatment, the callus had the lowest browning rate of 19.45% and the highest proliferation rate of 266.98%, and the morphology of the callus was friable. This study lays a foundation for future research in molecular biology and genetic improvement in Chinese kale.
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Claims 7
Claim · model-written summary
The proliferation medium with 20 g·L−1 sucrose gave the highest callus proliferation rate, 213.5%, among the sucrose concentrations tested.
- Taxon
- Chinese kale
Brassica oleracea var. alboglabrae0 - Material
- Cutiaoyusune0
- Organ / stage
- hypocotyle0
- Treatment
- proliferation medium with 20 g·L−1 sucrosee2
- Outcome
- proliferation ratee2
- Direction
- higheste2
- Value
- 213.5%e2
- Comparator
- different concentrations of sucrosee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hypocotyl of Chinese kale 'Cutiaoyusun' was used as explants”
e1“the effects of the different concentrations of sucrose and different browning inhibitors”
e2“the proliferation medium with 20 g·L−1 sucrose had the best effects on the proliferation and anti-browning of Chinese kale callus, and its proliferation rate was the highest, reaching 213.5%”
Claim · model-written summary
The 20 g·L−1 sucrose medium gave a browning rate as low as 27.77%, the lowest reported for sucrose concentrations.
- Taxon
- Chinese kaleBrassica oleracea var. alboglabrae0
- Material
- Cutiaoyusune0
- Organ / stage
- hypocotyle0
- Treatment
- proliferation medium with 20 g·L−1 sucrosee2
- Outcome
- browning ratee3
- Direction
- as low ase3
- Value
- 27.77%e3
- Comparator
- different concentrations of sucrosee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hypocotyl of Chinese kale 'Cutiaoyusun' was used as explants”
e1“the effects of the different concentrations of sucrose and different browning inhibitors”
e2“the proliferation medium with 20 g·L−1 sucrose had the best effects on the proliferation and anti-browning of Chinese kale callus, and its proliferation rate was the highest, reaching 213.5%”
e3“the browning rate was as low as 27.77%”
Claim · model-written summary
Adding 0.2 g·L−1 ascorbic acid to the culture medium significantly promoted callus proliferation.
- Taxon
- Chinese kaleBrassica oleracea var. alboglabrae0
- Material
- Cutiaoyusune0
- Organ / stage
- hypocotyle0
- Treatment
- adding 0.2 g·L−1 ascorbic acid to the culture mediume4
- Outcome
- callus proliferatione4
- Direction
- promotee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hypocotyl of Chinese kale 'Cutiaoyusun' was used as explants”
e4“adding 0.2 g·L−1 ascorbic acid to the culture medium can significantly promote callus proliferation and reduce browning rate”
Claim · model-written summary
Adding 0.2 g·L−1 ascorbic acid to the culture medium significantly reduced the callus browning rate.
- Taxon
- Chinese kaleBrassica oleracea var. alboglabrae0
- Material
- Cutiaoyusune0
- Organ / stage
- hypocotyle0
- Treatment
- adding 0.2 g·L−1 ascorbic acid to the culture mediume4
- Outcome
- browning ratee4
- Direction
- reducee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hypocotyl of Chinese kale 'Cutiaoyusun' was used as explants”
e4“adding 0.2 g·L−1 ascorbic acid to the culture medium can significantly promote callus proliferation and reduce browning rate”
Claim · model-written summary
In the ascorbic acid treatment the callus had the lowest browning rate, 19.45%.
- Taxon
- Chinese kaleBrassica oleracea var. alboglabrae0
- Material
- Cutiaoyusune0
- Organ / stage
- calluse5
- Treatment
- adding 0.2 g·L−1 ascorbic acid to the culture mediume4
- Outcome
- browning ratee5
- Direction
- loweste5
- Value
- 19.45%e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hypocotyl of Chinese kale 'Cutiaoyusun' was used as explants”
e4“adding 0.2 g·L−1 ascorbic acid to the culture medium can significantly promote callus proliferation and reduce browning rate”
e5“In this treatment, the callus had the lowest browning rate of 19.45% and the highest proliferation rate of 266.98%, and the morphology of the callus was friable”
Claim · model-written summary
In the ascorbic acid treatment the callus had the highest proliferation rate, 266.98%.
- Taxon
- Chinese kaleBrassica oleracea var. alboglabrae0
- Material
- Cutiaoyusune0
- Organ / stage
- calluse5
- Treatment
- adding 0.2 g·L−1 ascorbic acid to the culture mediume4
- Outcome
- proliferation ratee5
- Direction
- higheste5
- Value
- 266.98%e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hypocotyl of Chinese kale 'Cutiaoyusun' was used as explants”
e4“adding 0.2 g·L−1 ascorbic acid to the culture medium can significantly promote callus proliferation and reduce browning rate”
e5“In this treatment, the callus had the lowest browning rate of 19.45% and the highest proliferation rate of 266.98%, and the morphology of the callus was friable”
Claim · model-written summary
In the ascorbic acid treatment the callus morphology was friable.
- Taxon
- Chinese kaleBrassica oleracea var. alboglabrae0
- Material
- Cutiaoyusune0
- Organ / stage
- calluse5
- Treatment
- adding 0.2 g·L−1 ascorbic acid to the culture mediume4
- Outcome
- morphology of the calluse5
- Qualifier
- friablee5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hypocotyl of Chinese kale 'Cutiaoyusun' was used as explants”
e4“adding 0.2 g·L−1 ascorbic acid to the culture medium can significantly promote callus proliferation and reduce browning rate”
e5“In this treatment, the callus had the lowest browning rate of 19.45% and the highest proliferation rate of 266.98%, and the morphology of the callus was friable”
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See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.