Abstract
Monoacylglycerol lipase (MAGL) hydrolyzes monoacylglycerol, producing free fatty acid and glycerol. Although this enzyme has been shown to play important roles in mammal, its potential function in plants remains poorly understood. In a survey of the MAGL genes in Brassica napus, we found tapetal expression of BnaC.MAGL8.a, a homolog of AtMAGL8, results in male sterility in Arabidopsis thaliana. Retarded tapetal PCD and defected pollen wall were observed in the transgenic plants. The tapetal cells became vacuolated at stage 9, and then degenerated at stage 11. Most microspores degenerated with the tapetal cells, and only few pollen grains with an irregular-shaped exine layer were produced in the transgenic plants. Transcriptome analysis identified 398 differentially expressed genes. Most of them are involved in pollen development and stress response. ABORTED MICROSPORES and its downstream pollen wall biosynthesis genes were down-regulated, but genes related with reactive oxygen species homeostasis and jasmonates signalling were up-regulated in the transgenic plants. These results suggest that expression of BnaC.MAGL8.a in tapetum invokes stress response and impairs pollen development. The apparent phenotypic similarity between atgpat1 mutant and BnA9::BnaC.MAGL8.a transgenic plants lead us to propose a role for monoacylglycerol (MAG) in pollen development in Arabidopsis. Our study provides insights on not only the biological function of plant MAGL genes but also the role of MAG in pollen development.
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Claims 13
Claim · model-written summary
Tapetal BnaC.MAGL8.a expression caused male sterility in Arabidopsis thaliana.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- tapetal expression of BnaC.MAGL8.ae0
- Gene
- BnaC.MAGL8.ae0
- Outcome
- male sterilitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tapetal expression of BnaC.MAGL8.a, a homolog of AtMAGL8, results in male sterility in Arabidopsis thaliana”
Claim · model-written summary
Tapetal programmed cell death was retarded in the transgenic plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- transgenic plantse0
- Organ / stage
- tapetale0
- Outcome
- tapetal PCDe0
- Direction
- Retardede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Retarded tapetal PCD and defected pollen wall were observed in the transgenic plants.”
t0“Arabidopsis”
Claim · model-written summary
Transgenic plants had defective pollen walls.
- Taxon
- ArabidopsisArabidopsist0
- Material
- transgenic plantse0
- Organ / stage
- pollen walle0
- Outcome
- defected pollen walle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Retarded tapetal PCD and defected pollen wall were observed in the transgenic plants.”
t0“Arabidopsis”
Claim · model-written summary
Tapetal cells became vacuolated at stage 9.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- tapetal cellse0
- Outcome
- became vacuolatede0
- Qualifier
- stage 9e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The tapetal cells became vacuolated at stage 9”
t0“Arabidopsis”
Claim · model-written summary
Tapetal cells degenerated at stage 11.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- tapetal cellse1
- Outcome
- degeneratede0
- Qualifier
- stage 11e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“then degenerated at stage 11”
e1“The tapetal cells became vacuolated at stage 9, and then degenerated at stage 11.”
t0“Arabidopsis”
Claim · model-written summary
Most microspores degenerated along with tapetal cells.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- microsporese0
- Outcome
- Most microspores degeneratede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most microspores degenerated with the tapetal cells”
t0“Arabidopsis”
Claim · model-written summary
Only a few pollen grains with irregular exine were produced in transgenic plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- transgenic plantse0
- Organ / stage
- pollen grainse0
- Outcome
- only few pollen grains with an irregular-shaped exine layer were producede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only few pollen grains with an irregular-shaped exine layer were produced in the transgenic plants”
t0“Arabidopsis”
Claim · model-written summary
Transcriptome analysis found 398 differentially expressed genes.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- differentially expressed genese0
- Value
- 398e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome analysis identified 398 differentially expressed genes.”
t0“Arabidopsis”
Claim · model-written summary
Most differentially expressed genes were involved in pollen development and stress response.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- differentially expressed genese1
- Outcome
- involved in pollen development and stress responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most of them are involved in pollen development and stress response.”
e1“Transcriptome analysis identified 398 differentially expressed genes.”
t0“Arabidopsis”
Claim · model-written summary
ABORTED MICROSPORES and downstream pollen wall biosynthesis genes were down-regulated.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ABORTED MICROSPORESe0
- Outcome
- downstream pollen wall biosynthesis genese0
- Direction
- down-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABORTED MICROSPORES and its downstream pollen wall biosynthesis genes were down-regulated”
t0“Arabidopsis”
Claim · model-written summary
Reactive oxygen species homeostasis and jasmonate signaling genes were up-regulated.
- Taxon
- ArabidopsisArabidopsist0
- Material
- transgenic plantse0
- Outcome
- genes related with reactive oxygen species homeostasis and jasmonates signallinge0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes related with reactive oxygen species homeostasis and jasmonates signalling were up-regulated in the transgenic plants”
t0“Arabidopsis”
Claim · model-written summary
The authors infer that tapetal BnaC.MAGL8.a expression elicits stress response and impairs pollen development.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- tapetume0
- Gene
- BnaC.MAGL8.ae0
- Outcome
- impairs pollen developmente0
- Qualifier
- invokes stress responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of BnaC.MAGL8.a in tapetum invokes stress response and impairs pollen development”
t0“Arabidopsis”
Claim · model-written summary
Phenotypic similarity to atgpat1 led the authors to propose a role for monoacylglycerol in Arabidopsis pollen development.
- Taxon
- ArabidopsisArabidopsise0
- Material
- atgpat1 mutante0
- Gene
- BnA9::BnaC.MAGL8.ae0
- Compound
- monoacylglycerole0
- Outcome
- pollen developmente0
- Qualifier
- apparent phenotypic similaritye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The apparent phenotypic similarity between atgpat1 mutant and BnA9::BnaC.MAGL8.a transgenic plants lead us to propose a role for monoacylglycerol (MAG) in pollen development in Arabidopsis.”
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