Abstract
Hydrogen peroxide (H2O2) is a common signal molecule initiating transcriptional responses to all the known biotic and abiotic stresses of land plants. However, the degree of involvement of H2O2 in these stress responses has not yet been well studied. Here we identify time-dependent transcriptome profiles stimulated by H2O2 application in Arabidopsis (Arabidopsis thaliana) seedlings. Promoter prediction based on transcriptome data suggests strong crosstalk among high light, heat, and wounding stress responses in terms of environmental stresses and between the abscisic acid (ABA) and salicylic acid (SA) responses in terms of phytohormone signaling. Quantitative analysis revealed that ABA accumulation is induced by H2O2 but SA is not, suggesting that the implied crosstalk with ABA is achieved through ABA accumulation while the crosstalk with SA is different. We identified potential direct regulatory pairs between regulator transcription factor (TF) proteins and their regulated TF genes based on the time-course transcriptome analysis for the H2O2 response, in vivo regulation of the regulated TF by the regulator TF identified by expression analysis of mutants and overexpressors, and in vitro binding of the regulator TF protein to the target TF promoter. These analyses enabled the establishment of part of the transcriptional regulatory network for the H2O2 response composed of 15 regulatory pairs of TFs, including five pairs previously reported. This regulatory network is suggested to be involved in a wide range of biotic and abiotic stress responses in Arabidopsis.
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Claims 8
Claim · model-written summary
H2O2 induced time-dependent transcriptome profiles in Arabidopsis seedlings.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- seedlingse0
- Treatment
- H2O2 applicatione0
- Outcome
- time-dependent transcriptome profilese0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“time-dependent transcriptome profiles stimulated by H2O2 application in Arabidopsis (Arabidopsis thaliana) seedlings”
Claim · model-written summary
Promoter prediction suggested crosstalk among high light, heat and wounding responses.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- crosstalk among high light, heat, and wounding stress responsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Promoter prediction based on transcriptome data suggests strong crosstalk among high light, heat, and wounding stress responses”
t0“Arabidopsis (Arabidopsis thaliana) seedlings”
Claim · model-written summary
Promoter prediction suggested crosstalk between ABA and SA signaling responses.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- abscisic acid (ABA) and salicylic acid (SA) responsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“between the abscisic acid (ABA) and salicylic acid (SA) responses in terms of phytohormone signaling”
t0“Arabidopsis (Arabidopsis thaliana) seedlings”
Claim · model-written summary
H2O2 induced ABA accumulation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- H2O2e0
- Outcome
- ABA accumulatione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA accumulation is induced by H2O2”
t0“Arabidopsis (Arabidopsis thaliana) seedlings”
Claim · model-written summary
H2O2 did not induce SA accumulation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- H2O2e0
- Outcome
- SAe0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA accumulation is induced by H2O2 but SA is not”
t0“Arabidopsis (Arabidopsis thaliana) seedlings”
Claim · model-written summary
The study identified candidate direct TF regulatory pairs for the H2O2 response.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- transcription factor (TF) proteinse0
- Outcome
- potential direct regulatory pairse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified potential direct regulatory pairs between regulator transcription factor (TF) proteins and their regulated TF genes”
t0“Arabidopsis (Arabidopsis thaliana) seedlings”
Claim · model-written summary
A partial H2O2 regulatory network contained 15 TF pairs, including five previously reported pairs.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- transcriptional regulatory network for the H2O2 responsee0
- Value
- 15 regulatory pairs of TFse0
- Qualifier
- five pairs previously reportede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcriptional regulatory network for the H2O2 response composed of 15 regulatory pairs of TFs, including five pairs previously reported”
t0“Arabidopsis (Arabidopsis thaliana) seedlings”
Claim · model-written summary
Authors suggest the network participates in broad biotic and abiotic stress responses.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- biotic and abiotic stress responsese0
- Qualifier
- This regulatory networke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This regulatory network is suggested to be involved in a wide range of biotic and abiotic stress responses in Arabidopsis”
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