Abstract
Expression of the gdh2 gene encoding the alpha-subunit of mitochondrial glutamate dehydrogenase depends on redox state of the mitochondrial electron transport chain. Treatment of Arabidopsis thaliana cell suspension with antimycin A, a respiratory chain complex III inhibitor, resulted in an increase in gdh2 transcripts within 2 h. Inhibition of complex I by rotenone did not influence the transcript level, but treatment with potassium cyanide, a complex IV inhibitor, also increased the transcript content. Thus, gdh2 gene expression obviously responds to changes in the respiratory chain segment localized between complexes I and III. Lack of activation of gene expression after treatment of a cell suspension with hydrogen peroxide and the prooxidant paraquat and results of experiments with antioxidants suggest that gdh2 gene expression is not associated with increased content of reactive oxygen species generated during inhibition of the electron transport chain. Protein phosphorylation by serine/threonine protein kinases is the essential step required for signal transduction into nucleus resulting in the induction of gdh2 expression.
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Claims 8
Claim · model-written summary
Antimycin A increased Arabidopsis gdh2 transcripts within two hours.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- antimycin Ae0
- Gene
- gdh2e0
- Outcome
- gdh2 transcriptse0
- Direction
- increasee0
- Value
- 2 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of Arabidopsis thaliana cell suspension with antimycin A, a respiratory chain complex III inhibitor, resulted in an increase in gdh2 transcripts within 2 h”
Claim · model-written summary
Rotenone inhibition of complex I did not affect gdh2 transcript level.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- rotenonee0
- Gene
- gdh2t0
- Outcome
- transcript levele0
- Direction
- did not influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of complex I by rotenone did not influence the transcript level”
t0“Induction of Arabidopsis gdh2 gene expression during changes in redox state of the mitochondrial respiratory chain”
Claim · model-written summary
Potassium cyanide increased gdh2 transcript content.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- potassium cyanidee0
- Gene
- gdh2t0
- Outcome
- transcript contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with potassium cyanide, a complex IV inhibitor, also increased the transcript content”
t0“Induction of Arabidopsis gdh2 gene expression during changes in redox state of the mitochondrial respiratory chain”
Claim · model-written summary
hydrogen peroxide did not activate gdh2 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- hydrogen peroxidee0
- Gene
- gdh2t0
- Outcome
- gene expressione0
- Direction
- Lack of activatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lack of activation of gene expression after treatment of a cell suspension with hydrogen peroxide and the prooxidant paraquat”
t0“Induction of Arabidopsis gdh2 gene expression during changes in redox state of the mitochondrial respiratory chain”
Claim · model-written summary
paraquat did not activate gdh2 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- paraquate0
- Gene
- gdh2t0
- Outcome
- gene expressione0
- Direction
- Lack of activatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lack of activation of gene expression after treatment of a cell suspension with hydrogen peroxide and the prooxidant paraquat”
t0“Induction of Arabidopsis gdh2 gene expression during changes in redox state of the mitochondrial respiratory chain”
Claim · model-written summary
gdh2 expression responds to respiratory chain changes between complexes I and III.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- gdh2e0
- Outcome
- gdh2 gene expressione0
- Qualifier
- changes in the respiratory chain segment localized between complexes I and IIIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gdh2 gene expression obviously responds to changes in the respiratory chain segment localized between complexes I and III”
t0“Arabidopsis”
Claim · model-written summary
gdh2 expression was interpreted as unrelated to increased reactive oxygen species during respiratory inhibition.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- gdh2e0
- Outcome
- gdh2 gene expressione0
- Qualifier
- increased content of reactive oxygen speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gdh2 gene expression is not associated with increased content of reactive oxygen species generated during inhibition of the electron transport chain”
t0“Arabidopsis”
Claim · model-written summary
Serine/threonine protein kinase phosphorylation is required for induction of gdh2 expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- serine/threonine protein kinasese0
- Outcome
- induction of gdh2 expressione0
- Qualifier
- Protein phosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protein phosphorylation by serine/threonine protein kinases is the essential step required for signal transduction into nucleus resulting in the induction of gdh2 expression”
t0“Arabidopsis”
The same questions in other papers
hydrogen peroxide · molecular: expression · ArabidopsisPositive and negative results
3 papers in the same direction as this paper · 13 in the opposite direction
- positive
- positive
- positive
13 papers more in this group
See all 17 papers →rotenone · molecular: expression · ArabidopsisResults agree
1 paper in the same direction as this paper
- no effect
paraquat · molecular: expression · ArabidopsisResults agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.