Abstract
The functions of microRNA156 (miR156) and its targeted SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factor genes in plant development have been widely investigated. However, the role of the miR156/SPLs regulatory network in plant immune systems remains obscure. Here, we found that the accumulation of reactive oxygen species (ROS) and the transcripts of basal salicylic acid (SA) signaling pathway genes were lower in Arabidopsis Pro35S:MIR156 seedlings (miR156 overexpression mutants) but higher in Pro35S:MIM156 (miR156 repression mutants) and ProSPL9:rSPL9 (SPL9 overexpression mutants) seedlings compared with wild-type Col-0 plants (WT). As a result, Pro35S:MIR156 mutants induced greater susceptibility to Pseudomonas syringae pv. tomato DC3000 following syringe infiltration than WT, while Pro35S:MIM156 and ProSPL9:rSPL9 mutants showed enhanced resistance. In addition, foliar H 2 O 2 application resulted in activation of SA-mediated defense response and ablation of miR156-induced susceptibility to P. syringae pv. tomato DC3000 infection. Collectively, our results provide new insights into the function of the miR156/SPL network in Arabidopsis immune response by regulating ROS accumulation and activating the SA signaling pathway.
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Claims 8
Claim · model-written summary
Pro35S:MIR156 seedlings had lower ROS accumulation and lower basal SA signalling gene transcripts than wild-type Col-0.
- Taxon
- Arabidopsis
Arabidopsise1 - Material
- Pro35S:MIR156 seedlingse1
- Treatment
- miR156 overexpressione1
- Outcome
- accumulation of reactive oxygen species (ROS) and the transcripts of basal salicylic acid (SA) signaling pathway genese1
- Direction
- lowere1
- Comparator
- wild-type Col-0 plants (WT)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the accumulation of reactive oxygen species (ROS) and the transcripts of basal salicylic acid (SA) signaling pathway genes were lower in Arabidopsis Pro35S:MIR156 seedlings (miR156 overexpression mutants) but higher in Pro35S:MIM156 (miR156 repression mutants) and ProSPL9:rSPL9 (SPL9 overexpression mutants) seedlings compared with wild-type Col-0 plants (WT)”
Claim · model-written summary
Pro35S:MIM156 (miR156 repression) seedlings had higher ROS accumulation and higher basal SA signalling gene transcripts than wild-type Col-0.
- Taxon
- ArabidopsisArabidopsise1
- Material
- Pro35S:MIM156e1
- Treatment
- miR156 repressione1
- Outcome
- accumulation of reactive oxygen species (ROS) and the transcripts of basal salicylic acid (SA) signaling pathway genese1
- Direction
- highere1
- Comparator
- wild-type Col-0 plants (WT)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the accumulation of reactive oxygen species (ROS) and the transcripts of basal salicylic acid (SA) signaling pathway genes were lower in Arabidopsis Pro35S:MIR156 seedlings (miR156 overexpression mutants) but higher in Pro35S:MIM156 (miR156 repression mutants) and ProSPL9:rSPL9 (SPL9 overexpression mutants) seedlings compared with wild-type Col-0 plants (WT)”
Claim · model-written summary
ProSPL9:rSPL9 (SPL9 overexpression) seedlings had higher ROS accumulation and higher basal SA signalling gene transcripts than wild-type Col-0.
- Taxon
- ArabidopsisArabidopsise1
- Material
- ProSPL9:rSPL9e1
- Treatment
- SPL9 overexpressione1
- Outcome
- accumulation of reactive oxygen species (ROS) and the transcripts of basal salicylic acid (SA) signaling pathway genese1
- Direction
- highere1
- Comparator
- wild-type Col-0 plants (WT)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the accumulation of reactive oxygen species (ROS) and the transcripts of basal salicylic acid (SA) signaling pathway genes were lower in Arabidopsis Pro35S:MIR156 seedlings (miR156 overexpression mutants) but higher in Pro35S:MIM156 (miR156 repression mutants) and ProSPL9:rSPL9 (SPL9 overexpression mutants) seedlings compared with wild-type Col-0 plants (WT)”
Claim · model-written summary
Pro35S:MIR156 mutants were more susceptible to Pseudomonas syringae pv. tomato DC3000 than wild-type after syringe infiltration.
- Taxon
- ArabidopsisArabidopsise1
- Material
- Pro35S:MIR156 mutantse2
- Treatment
- syringe infiltratione2
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e2pathogen
- Outcome
- susceptibility to Pseudomonas syringae pv. tomato DC3000e2
- Direction
- greatere2
- Comparator
- WTe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the accumulation of reactive oxygen species (ROS) and the transcripts of basal salicylic acid (SA) signaling pathway genes were lower in Arabidopsis Pro35S:MIR156 seedlings (miR156 overexpression mutants) but higher in Pro35S:MIM156 (miR156 repression mutants) and ProSPL9:rSPL9 (SPL9 overexpression mutants) seedlings compared with wild-type Col-0 plants (WT)”
e2“Pro35S:MIR156 mutants induced greater susceptibility to Pseudomonas syringae pv. tomato DC3000 following syringe infiltration than WT”
Claim · model-written summary
Pro35S:MIM156 (miR156 repression) mutants showed enhanced resistance to Pseudomonas syringae pv. tomato DC3000 compared with WT.
- Taxon
- ArabidopsisArabidopsise1
- Material
- Pro35S:MIM156e3
- Treatment
- miR156 repressione1
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e2pathogen
- Outcome
- resistancee3
- Direction
- enhancede3
- Comparator
- WTe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the accumulation of reactive oxygen species (ROS) and the transcripts of basal salicylic acid (SA) signaling pathway genes were lower in Arabidopsis Pro35S:MIR156 seedlings (miR156 overexpression mutants) but higher in Pro35S:MIM156 (miR156 repression mutants) and ProSPL9:rSPL9 (SPL9 overexpression mutants) seedlings compared with wild-type Col-0 plants (WT)”
e2“Pro35S:MIR156 mutants induced greater susceptibility to Pseudomonas syringae pv. tomato DC3000 following syringe infiltration than WT”
e3“than WT, while Pro35S:MIM156 and ProSPL9:rSPL9 mutants showed enhanced resistance”
Claim · model-written summary
ProSPL9:rSPL9 (SPL9 overexpression) mutants showed enhanced resistance to Pseudomonas syringae pv. tomato DC3000 compared with WT.
- Taxon
- ArabidopsisArabidopsise1
- Material
- ProSPL9:rSPL9e3
- Treatment
- SPL9 overexpressione1
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e2pathogen
- Outcome
- resistancee3
- Direction
- enhancede3
- Comparator
- WTe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the accumulation of reactive oxygen species (ROS) and the transcripts of basal salicylic acid (SA) signaling pathway genes were lower in Arabidopsis Pro35S:MIR156 seedlings (miR156 overexpression mutants) but higher in Pro35S:MIM156 (miR156 repression mutants) and ProSPL9:rSPL9 (SPL9 overexpression mutants) seedlings compared with wild-type Col-0 plants (WT)”
e2“Pro35S:MIR156 mutants induced greater susceptibility to Pseudomonas syringae pv. tomato DC3000 following syringe infiltration than WT”
e3“than WT, while Pro35S:MIM156 and ProSPL9:rSPL9 mutants showed enhanced resistance”
Claim · model-written summary
Foliar H2O2 application activated SA-mediated defense response in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- foliar H 2 O 2 applicatione4
- Outcome
- activation of SA-mediated defense responsee4
- Direction
- activatione4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis Pro35S:MIR156 seedlings”
e4“foliar H 2 O 2 application resulted in activation of SA-mediated defense response and ablation of miR156-induced susceptibility to P. syringae pv. tomato DC3000 infection”
Claim · model-written summary
Foliar H2O2 application ablated miR156-induced susceptibility of Arabidopsis to Pseudomonas syringae pv. tomato DC3000 infection.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- foliar H 2 O 2 applicatione4
- Interactor
- P. syringae pv. tomato DC3000
Pseudomonas syringae pv. tomatoe4pathogen - Outcome
- miR156-induced susceptibility to P. syringae pv. tomato DC3000 infectione4
- Direction
- ablatione4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis Pro35S:MIR156 seedlings”
e4“foliar H 2 O 2 application resulted in activation of SA-mediated defense response and ablation of miR156-induced susceptibility to P. syringae pv. tomato DC3000 infection”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.