Abstract
Treatment of pea stem segments with indoleacetic acid (IAA) causes within 1 hour a 2- to 4-fold increase in activity of particulate uridine diphosphoglucose-dependent p-glucan synthetase obtainable from the tissue. The IAA effect is observable in tissue from all parts of the elongation zone of the pea stem, and also in older tissue that is not capable of a cell enlargement response to IAA. A large increase in activity is caused by IAA only if synthetase activity in the isolated tissue has first been allowed to fail substantially below the intact plant level, and only if sucrose is supplied along with IAA. Treatment of tissue with sucrose alone after a period of sugar starvation causes a transient rise of synthetase activity. The decline in synthetase activity in absence of IAA, the rise caused by IAA, and the transient rise caused by sucrose are all strongly temperature-dependent. IAA and sucrose do not affect the activity of isolated synthetase particles. Synthetase activity in vivo is sensitive to as low as 0.1 M IAA and is increased by IAA analogues that are active as auxins on elongation but not by nonauxin analogues. Activity begins to rise 10 to 15 minutes after exposure to IAA, which places this among the most rapid enzyme effects of a plant growth regulator heretofore demonstrated, and among the most rapid known metabolic effects of auxins. The effect is seen also with polysaccharide synthetase activity using uridine diphosphate-galactose or uridine diphosphate-xylose as substrates, and to a lesser extent with guanosine diphosphoglucose-dependent glucan synthetase activity. Glucan synthetase from IAA-treated tissue appears to have a higher affinity for uridine diphosphate-glucose than the control.
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Claims 14
Claim · model-written summary
IAA raised particulate glucan synthetase activity two- to fourfold within one hour.
- Taxon
- pea
Lathyrus oleraceuse0 - Organ / stage
- stem segmentse0
- Treatment
- indoleacetic acid (IAA)e0
- Outcome
- activity of particulate uridine diphosphoglucose-dependent p-glucan synthetasee0
- Direction
- increasee0
- Value
- 2- to 4-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of pea stem segments with indoleacetic acid (IAA) causes within 1 hour a 2- to 4-fold increase in activity of particulate uridine diphosphoglucose-dependent p-glucan synthetase obtainable from the tissue”
Claim · model-written summary
The IAA effect occurred throughout the elongation zone and in older tissue unable to enlarge in response to IAA.
- Taxon
- peaLathyrus oleraceuse0
- Organ / stage
- tissue from all parts of the elongation zone of the pea stem, and also in older tissuee0
- Outcome
- IAA effect is observablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The IAA effect is observable in tissue from all parts of the elongation zone of the pea stem, and also in older tissue that is not capable of a cell enlargement response to IAA”
Claim · model-written summary
A large IAA increase required prior loss of isolated tissue synthetase activity and sucrose supply.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- IAAe0
- Outcome
- large increase in activitye0
- Qualifier
- only if sucrose is supplied along with IAAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A large increase in activity is caused by IAA only if synthetase activity in the isolated tissue has first been allowed to fail substantially below the intact plant level, and only if sucrose is supplied along with IAA”
t0“Pea Stem Tissue”
Claim · model-written summary
Sucrose alone caused a transient synthetase activity rise after sugar starvation.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- sucrose alonee0
- Outcome
- synthetase activitye0
- Direction
- risee0
- Qualifier
- after a period of sugar starvatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of tissue with sucrose alone after a period of sugar starvation causes a transient rise of synthetase activity”
t0“Pea Stem Tissue”
Claim · model-written summary
Synthetase activity changes without IAA, with IAA, and with sucrose were strongly temperature dependent.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- IAAe0
- Outcome
- synthetase activitye0
- Qualifier
- strongly temperature-dependente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The decline in synthetase activity in absence of IAA, the rise caused by IAA, and the transient rise caused by sucrose are all strongly temperature-dependent”
t0“Pea Stem Tissue”
Claim · model-written summary
IAA and sucrose did not alter isolated synthetase particle activity.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- IAA and sucrosee0
- Outcome
- activity of isolated synthetase particlese0
- Direction
- do not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA and sucrose do not affect the activity of isolated synthetase particles”
t0“Pea Stem Tissue”
Claim · model-written summary
In vivo synthetase activity responded to IAA as low as 0.1 M.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- 0.1 M IAAe0
- Outcome
- Synthetase activity in vivoe0
- Direction
- sensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Synthetase activity in vivo is sensitive to as low as 0.1 M IAA”
t0“Pea Stem Tissue”
Claim · model-written summary
Auxin-active IAA analogues increased synthetase activity.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- IAA analogues that are active as auxins on elongatione0
- Outcome
- Synthetase activity in vivoe1
- Direction
- increasede0
- Comparator
- nonauxin analoguese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is increased by IAA analogues that are active as auxins on elongation but not by nonauxin analogues”
e1“Synthetase activity in vivo”
t0“Pea Stem Tissue”
Claim · model-written summary
Nonauxin analogues did not increase synthetase activity.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- nonauxin analoguese0
- Outcome
- Synthetase activity in vivoe1
- Comparator
- IAA analogues that are active as auxins on elongatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is increased by IAA analogues that are active as auxins on elongation but not by nonauxin analogues”
e1“Synthetase activity in vivo”
t0“Pea Stem Tissue”
Claim · model-written summary
Synthetase activity began rising 10 to 15 minutes after IAA exposure.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activity begins to rise 10 to 15 minutes after exposure to IAA”
t0“Pea Stem Tissue”
Claim · model-written summary
IAA also affected polysaccharide synthetase activity with uridine diphosphate-galactose as substrate.
- Taxon
- PeaLathyrus oleraceust0
- Gene
- polysaccharide synthetasee0
- Outcome
- polysaccharide synthetase activitye0
- Qualifier
- uridine diphosphate-galactosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect is seen also with polysaccharide synthetase activity using uridine diphosphate-galactose or uridine diphosphate-xylose as substrates”
t0“Pea Stem Tissue”
Claim · model-written summary
IAA also affected polysaccharide synthetase activity with uridine diphosphate-xylose as substrate.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- polysaccharide synthetase activitye0
- Qualifier
- uridine diphosphate-xylosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect is seen also with polysaccharide synthetase activity using uridine diphosphate-galactose or uridine diphosphate-xylose as substrates”
t0“Pea Stem Tissue”
Claim · model-written summary
IAA had a lesser effect on guanosine diphosphoglucose-dependent glucan synthetase activity.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- guanosine diphosphoglucose-dependent glucan synthetase activitye0
- Direction
- lesser extente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and to a lesser extent with guanosine diphosphoglucose-dependent glucan synthetase activity”
t0“Pea Stem Tissue”
Claim · model-written summary
Glucan synthetase from IAA-treated tissue appeared to have higher affinity for uridine diphosphate-glucose than controls.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- IAA-treated tissuee0
- Outcome
- affinity for uridine diphosphate-glucosee0
- Direction
- highere0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glucan synthetase from IAA-treated tissue appears to have a higher affinity for uridine diphosphate-glucose than the control”
t0“Pea Stem Tissue”
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