Paper · 1996 · Proceedings of the Florida State Horticultural Society

METHODS FOR EVALUATION OF SPRAY CHEMICAL PHYTOTOXICITY TO CITRUS

L. G. Albrigo

7
claims
12
verbatim quotes
5
citations
4
research areas
—
Not open access

Abstract

For Florida citrus, pesticides, nutritionals and growth regulators are often sprayed together in tank mixes in order to reduce sprayer use when timings for efficacy are relatively coincident. Many individual spray components are marginally phytotoxic and can result in spray burns when used together or if applied with adjuvants that increase absorption. The toxicity level of many standard spray materials is unknown and new products are not routinely tested for phytotoxicity in citrus, particularly as tank mixes with other pesticides or nutritionals. Three test methods were developed to allow spray chemical phytotoxicity testing using cell cultures, peel disks and whole fruit. Cell suspension cultures initiated from 'nucellar derived' embryonic callus of 'Hamlin' sweet orange and 2) peel disks of orange or grapefruit were grown in culture and exposed to media incorporated test chemicals. Reduced weight gain (Method 1) or color changes and callus growth reduction (Method 2) were used to evaluate phytotoxicity. Dilute sprays and droplet applications to on-tree fruit (Method 3) were used to evaluate individual and combinations of chemicals with and without spray adjuvants. The three tests effectively determined thresholds for phytotoxicity and will be useful for testing new citrus production chemicals. These were preliminary evaluations and could be refined for statistical application. Chemicals tested and found to have some level of phytotoxicity by these methods included Aliette, Morestan, Pro-Gibb with 2,4-D, citric, phosphorus, and phosphoric acids, and some additional pesticides in combination with urea. Two herbicide grade adjuvants tested (Herbex and Induce) increased phytotoxicity in on-tree tests. Salty water often reduced the influence of a surfactant. Acidity, in itself, was phytotoxic to the peel of the fruit indicating buffering is important to minimize spray burn.

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Claims 7

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Three tests determined citrus phytotoxicity thresholds.

Outcome
thresholds for phytotoxicitye0

Not reportedComparatorValue

Verbatim

e0

“The three tests effectively determined thresholds for phytotoxicity and will be useful for testing new citrus production chemicals.”

✓ located verbatim in the source · abstract, characters 1187–1318 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Aliette, Morestan, Pro-Gibb with 2,4-D, citric, phosphorus and phosphoric acids showed some phytotoxicity.

Treatment
Aliette, Morestan, Pro-Gibb with 2,4-D, citric, phosphorus, and phosphoric acidse0
Outcome
phytotoxicitye0

Not reportedTaxon

Verbatim

e0

“Chemicals tested and found to have some level of phytotoxicity by these methods included Aliette, Morestan, Pro-Gibb with 2,4-D, citric, phosphorus, and phosphoric acids, and some additional pesticides in combination with urea.”

✓ located verbatim in the source · abstract, characters 1404–1631 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Additional pesticides combined with urea showed some phytotoxicity.

Treatment
additional pesticides in combination with ureae0
Outcome
phytotoxicitye1

Not reportedTaxon

Verbatim

e0

“some additional pesticides in combination with urea”

✓ located verbatim in the source · abstract, characters 1579–1630 · show in text ↑
e1

“Chemicals tested and found to have some level of phytotoxicity”

✓ located verbatim in the source · abstract, characters 1404–1466 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Herbex and Induce increased phytotoxicity in on-tree citrus tests.

Taxon
CITRUSCitrust0
Treatment
Herbex and Inducee0
Outcome
phytotoxicitye0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus
Citrus · Taxon · picture of the genus (one of its species)Ellen Levy Finch ( Elf ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Two herbicide grade adjuvants tested (Herbex and Induce) increased phytotoxicity in on-tree tests.”

✓ located verbatim in the source · abstract, characters 1632–1730 · show in text ↑
t0

“CITRUS”

✓ located verbatim in the source · title, characters 58–64 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Salty water often reduced surfactant influence.

Taxon
CITRUSCitrust0
Treatment
Salty watere0
Outcome
influence of a surfactante0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus
Citrus · Taxon · picture of the genus (one of its species)Ellen Levy Finch ( Elf ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Salty water often reduced the influence of a surfactant.”

✓ located verbatim in the source · abstract, characters 1731–1787 · show in text ↑
t0

“CITRUS”

✓ located verbatim in the source · title, characters 58–64 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Acidity itself was phytotoxic to citrus fruit peel.

Taxon
CITRUSCitrust0
Organ / stage
peel of the fruite0
Treatment
Aciditye0
Outcome
phytotoxic to the peel of the fruite0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus
Citrus · Taxon · picture of the genus (one of its species)Ellen Levy Finch ( Elf ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Acidity, in itself, was phytotoxic to the peel of the fruit indicating buffering is important to minimize spray burn.”

✓ located verbatim in the source · abstract, characters 1788–1905 · show in text ↑
t0

“CITRUS”

✓ located verbatim in the source · title, characters 58–64 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors suggest buffering to minimize citrus spray burn.

Taxon
CITRUSCitrust0
Outcome
minimize spray burne0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus
Citrus · Taxon · picture of the genus (one of its species)Ellen Levy Finch ( Elf ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“buffering is important to minimize spray burn”

✓ located verbatim in the source · abstract, characters 1859–1904 · show in text ↑
t0

“CITRUS”

✓ located verbatim in the source · title, characters 58–64 · show in text ↑

salinity · molecular: metabolites · CitrusResults agree

6 papers: 0 positive, 6 negative or no effect

5 papers in the same direction as this paper

  1. no effect
  2. no effect
  3. negative

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.