Abstract
GRF interacting factors (GIFs) are involved in several developmental processes in Arabidopsis. We previously showed that upregulation of OsGIF1 expression improves rice grain size. However, whether OsGIF1 is involved in other developmental processes remains unclear. Here, we report pleiotropic effects of OsGIF1 on rice organ size regulation. Overexpression and functional knock-out via a CRISPR/Cas9 strategy revealed that OsGIF1 not only positively regulates the sizes of rice leaf, stem, and grain but also influences rice reproduction. Expression profiles based on both qRT-PCR and GUS (β-glucuronidase) histochemical staining suggested that OsGIF1 is differentially expressed across various rice tissues, consistent with its roles in regulating the development of multiple rice organs. Additionally, we found that OsGIF1-YFP localized preferentially in the nucleus, which supports its proposed role as a transcriptional cofactor. Further histological analysis suggested that OsGIF1 affected rice organ size possibly by regulating cell size. Our results suggest that OsGIF1 plays important roles in vegetative and reproductive developmental processes, with important implications for rice breeding.
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Claims 4
Claim · model-written summary
OsGIF1 positively regulates the sizes of rice leaf, stem and grain.
- Taxon
- rice
Oryza sativae0 - Treatment
- Overexpression and functional knock-out via a CRISPR/Cas9 strategye0
- Gene
- OsGIF1e0
- Outcome
- sizes of rice leaf, stem, and graine0
- Direction
- positively regulatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression and functional knock-out via a CRISPR/Cas9 strategy revealed that OsGIF1 not only positively regulates the sizes of rice leaf, stem, and grain but also influences rice reproduction”
Claim · model-written summary
OsGIF1 influences rice reproduction under overexpression and CRISPR/Cas9 knock-out.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression and functional knock-out via a CRISPR/Cas9 strategye0
- Gene
- OsGIF1e0
- Outcome
- rice reproductione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression and functional knock-out via a CRISPR/Cas9 strategy revealed that OsGIF1 not only positively regulates the sizes of rice leaf, stem, and grain but also influences rice reproduction”
Claim · model-written summary
OsGIF1 is differentially expressed across various rice tissues.
- Taxon
- riceOryza sativae0
- Organ / stage
- various rice tissuese0
- Gene
- OsGIF1e0
- Outcome
- differentially expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression profiles based on both qRT-PCR and GUS (β-glucuronidase) histochemical staining suggested that OsGIF1 is differentially expressed across various rice tissues”
Claim · model-written summary
OsGIF1 possibly affects rice organ size by regulating cell size.
- Taxon
- riceOryza sativae0
- Gene
- OsGIF1e0
- Outcome
- rice organ sizee0
- Qualifier
- regulating cell sizee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsGIF1 affected rice organ size possibly by regulating cell size”
The same questions in other papers
loss of function · organism · Oryza sativa (rice)Positive and negative results
6 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- no effect
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.