Abstract
Experiment on anther culture in sweet pepper (Capsicum annuum L.) hybrids Bharat and Indra was carried out to investigate the responses of three anther developmental stages on the production of androgenic/microspore derived calli, using MS medium supplemented with various growth regulators in different ratios and with or without pre-culture heat treatment of anthers at 35oC for seven days. In general, heat treated anthers resulted in increased callus induction percentage in all growth regulator combinations and flower bud developmental stages. Most promising results were obtained on MS medium supplemented with 1.0 mg/l zeatin along with 0.2 mg/l 2, 4-D and 15.0 mg/l AgNO3 with heat treatment reported maximum callus induction in both the hybrids, 54.02% and 60.92%, respectively when the anthers were at developmental stage-II (i.e. microspores at late uni-nucleate stage). Irrespective of anther developmental stage, zeatin was found superior in callus induction from cultured anthers followed by TDZ and Kinetin. Response of BAP was noted poor as compared to other cytokinins tested. The anther containing microspore at late uni-nucleate stage i.e. stage-II recorded maximum callus induction followed by anthers at stage-I having microspore at early uni-nucleate stage. The anthers at developmental stage-III, which contained mature pollen grains, were poor in callus production. Supplementation of MS medium with AgNO3 15.0 mg/l along with other growth regulators gave better results in all the stages of anther development.
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Claims 9
Claim · model-written summary
Heat pre-treatment of anthers at 35°C for seven days increased callus induction compared with no heat treatment.
- Taxon
- Capsicum annuum
Capsicum annuumt0 - Material
- Bharat and Indrae0
- Organ / stage
- antherse2
- Treatment
- pre-culture heat treatment of anthers at 35oC for seven dayse1
- Outcome
- callus induction percentagee2
- Direction
- increasede2
- Comparator
- without pre-culture heat treatmente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro Androgenesis in Capsicum (Capsicum annuum L.)”
e0“Experiment on anther culture in sweet pepper (Capsicum annuum L.) hybrids Bharat and Indra”
e1“with or without pre-culture heat treatment of anthers at 35oC for seven days”
e2“heat treated anthers resulted in increased callus induction percentage in all growth regulator combinations and flower bud developmental stages”
Claim · model-written summary
In hybrid Bharat, the maximum callus induction was 54.02% on zeatin-containing MS medium with heat treatment at anther stage-II.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Material
- Bharate0
- Organ / stage
- developmental stage-IIe3
- Treatment
- MS medium supplemented with 1.0 mg/l zeatin along with 0.2 mg/l 2, 4-D and 15.0 mg/l AgNO3 with heat treatmente3
- Outcome
- callus inductione3
- Direction
- maximume3
- Value
- 54.02%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro Androgenesis in Capsicum (Capsicum annuum L.)”
e0“Experiment on anther culture in sweet pepper (Capsicum annuum L.) hybrids Bharat and Indra”
e3“Most promising results were obtained on MS medium supplemented with 1.0 mg/l zeatin along with 0.2 mg/l 2, 4-D and 15.0 mg/l AgNO3 with heat treatment reported maximum callus induction in both the hybrids, 54.02% and 60.92%, respectively when the anthers were at developmental stage-II”
Claim · model-written summary
In hybrid Indra, the maximum callus induction was 60.92% on zeatin-containing MS medium with heat treatment at anther stage-II.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Material
- Indrae0
- Organ / stage
- developmental stage-IIe3
- Treatment
- MS medium supplemented with 1.0 mg/l zeatin along with 0.2 mg/l 2, 4-D and 15.0 mg/l AgNO3 with heat treatmente3
- Outcome
- callus inductione3
- Direction
- maximume3
- Value
- 60.92%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro Androgenesis in Capsicum (Capsicum annuum L.)”
e0“Experiment on anther culture in sweet pepper (Capsicum annuum L.) hybrids Bharat and Indra”
e3“Most promising results were obtained on MS medium supplemented with 1.0 mg/l zeatin along with 0.2 mg/l 2, 4-D and 15.0 mg/l AgNO3 with heat treatment reported maximum callus induction in both the hybrids, 54.02% and 60.92%, respectively when the anthers were at developmental stage-II”
Claim · model-written summary
Zeatin gave superior callus induction from cultured anthers compared with TDZ and Kinetin, regardless of anther stage.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Organ / stage
- antherse4
- Treatment
- zeatine4
- Outcome
- callus inductione4
- Direction
- superiore4
- Comparator
- TDZ and Kinetine4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro Androgenesis in Capsicum (Capsicum annuum L.)”
e4“Irrespective of anther developmental stage, zeatin was found superior in callus induction from cultured anthers followed by TDZ and Kinetin”
Claim · model-written summary
BAP gave a poor response compared with the other cytokinins tested.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Treatment
- BAPe5
- Outcome
- Responsee5
- Direction
- poore5
- Comparator
- other cytokinins testede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro Androgenesis in Capsicum (Capsicum annuum L.)”
e5“Response of BAP was noted poor as compared to other cytokinins tested”
Claim · model-written summary
Anthers containing late uni-nucleate microspores gave the maximum callus induction.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Organ / stage
- anther containing microspore at late uni-nucleate stagee6
- Outcome
- callus inductione6
- Direction
- maximume6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro Androgenesis in Capsicum (Capsicum annuum L.)”
e6“The anther containing microspore at late uni-nucleate stage i.e. stage-II recorded maximum callus induction followed by anthers at stage-I having microspore at early uni-nucleate stage”
Claim · model-written summary
Anthers at stage-I with early uni-nucleate microspores ranked second in callus induction.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Organ / stage
- anthers at stage-I having microspore at early uni-nucleate stagee6
- Outcome
- callus inductione6
- Direction
- followed bye6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro Androgenesis in Capsicum (Capsicum annuum L.)”
e6“The anther containing microspore at late uni-nucleate stage i.e. stage-II recorded maximum callus induction followed by anthers at stage-I having microspore at early uni-nucleate stage”
Claim · model-written summary
Anthers at developmental stage-III, containing mature pollen, were poor in callus production.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Organ / stage
- anthers at developmental stage-IIIe7
- Outcome
- callus productione7
- Direction
- poore7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro Androgenesis in Capsicum (Capsicum annuum L.)”
e7“The anthers at developmental stage-III, which contained mature pollen grains, were poor in callus production”
Claim · model-written summary
AgNO3 at 15.0 mg/l with other growth regulators gave better results at all anther stages.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Organ / stage
- all the stages of anther developmente7
- Treatment
- Supplementation of MS medium with AgNO3 15.0 mg/le7
- Outcome
- resultse7
- Direction
- bettere7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro Androgenesis in Capsicum (Capsicum annuum L.)”
e7“Supplementation of MS medium with AgNO3 15.0 mg/l along with other growth regulators gave better results in all the stages of anther development”
The same questions in other papers
zeatin · growth · Capsicum annuum (sweet pepper)Results agree
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- positive
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2,4-D · growth · Capsicum annuum (sweet pepper)Results agree
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- positive
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silver(1+) nitrate · growth · Capsicum annuum (sweet pepper)Results agree
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.