Paper · 2017 · PubMed

Synergistic effect of silver nitrate and coconut water on shoot differentiation and plant regeneration from cultured cotyledons of Capsicum annuum L.

J B Mythili; P R Rajeev; G Vinay; et al.

5
claims
10
verbatim quotes
10
citations
2
research areas
—
Not open access

Abstract

Chili pepper (Capsicum annuum L.) ranks among the most important vegetable crop belonging to the family Solanaceae that is consumed both as vegetable and spice throughout the world. C. annuum, as crop, in order to meet the target yield, demands improved variety that could overcome environmental challenges viz., biotic and abiotic stress. Cultivar improvement essentially requires an efficient in vitro regeneration protocol. In the present study, we investigated the influence of silver nitrate (AgNO3) and coconut water, individually as well in combination, on in vitro shoot elongation and plant regeneration from cotyledon explants of C. annuum cv G-4. Shoot buds were induced on shoot bud induction medium supplemented with either 44.38 µM 6-benzylaminopurine (BAP) or 9.0 µM thidiazuron (TDZ) along with 5.77 µM gibberellic acid (GA3) and 14.7 µM phenyl acetic acid (PAA). Elongation of shoot buds was obtained on elongation medium containing 8.87 µM BA or 0.45 µM TDZ, 5.77 µM GA3 and 14.7 µM PAA followed by rooting in 9.8 µM indole-3-butyric acid (IBA). All the media were supplemented with 30 µM AgNO3 and/or coconut water (10% v/v). The presence of coconut water in the elongation media enhanced the regeneration of well developed shoots from differentiating explants on TDZ media while AgNO3 resulted in enhanced production of rooted shoots with greater influence on emerging shoots from BAP media upon transfer to rooting media. There was synergistic response with further enhancement of elongated shoots/elongated rooted shoots on the combined use of coconut water and AgNO3. The elongation media produced significantly higher total shoots when AgNO3 was used synergistically with coconut water (59.0%) as against AgNO3 alone (38.0%). While in rooting media, there was significantly higher production of elongated rooted shoots when coconut water was used synergistically with AgNO3 (47.2%) as against the coconut water alone (14.4%).

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Coconut water in TDZ elongation media enhanced regeneration of developed shoots.

Taxon
Capsicum annuum L.Capsicum annuumt0
Organ / stage
shootse0
Treatment
coconut watere0
Outcome
regeneration of well developed shootse0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The presence of coconut water in the elongation media enhanced the regeneration of well developed shoots from differentiating explants on TDZ media”

✓ located verbatim in the source · abstract, characters 1145–1292 · show in text ↑
t0

“Capsicum annuum L.”

✓ located verbatim in the source · title, characters 131–149 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

AgNO3 enhanced rooted shoot production after BAP medium, particularly emerging shoots.

Taxon
Capsicum annuum L.Capsicum annuumt0
Treatment
AgNO3e0
Outcome
production of rooted shootse0
Direction
enhancede0
Qualifier
emerging shoots from BAP mediae0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“AgNO3 resulted in enhanced production of rooted shoots with greater influence on emerging shoots from BAP media upon transfer to rooting media”

✓ located verbatim in the source · abstract, characters 1299–1441 · show in text ↑
t0

“Capsicum annuum L.”

✓ located verbatim in the source · title, characters 131–149 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combined coconut water and AgNO3 further enhanced elongated shoots and elongated rooted shoots.

Taxon
Capsicum annuum L.Capsicum annuumt0
Treatment
combined use of coconut water and AgNO3e0
Outcome
elongated shoots/elongated rooted shootse0
Direction
enhancemente0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“There was synergistic response with further enhancement of elongated shoots/elongated rooted shoots on the combined use of coconut water and AgNO3.”

✓ located verbatim in the source · abstract, characters 1443–1590 · show in text ↑
t0

“Capsicum annuum L.”

✓ located verbatim in the source · title, characters 131–149 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

AgNO3 with coconut water produced 59.0% total shoots in elongation media versus 38.0% with AgNO3 alone.

Taxon
Capsicum annuum L.Capsicum annuumt0
Treatment
AgNO3 was used synergistically with coconut watere0
Outcome
total shootse0
Direction
highere0
Value
59.0%e0
Qualifier
38.0%e0
Comparator
AgNO3 alonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The elongation media produced significantly higher total shoots when AgNO3 was used synergistically with coconut water (59.0%) as against AgNO3 alone (38.0%).”

✓ located verbatim in the source · abstract, characters 1591–1749 · show in text ↑
t0

“Capsicum annuum L.”

✓ located verbatim in the source · title, characters 131–149 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Coconut water with AgNO3 produced 47.2% elongated rooted shoots in rooting media versus 14.4% with coconut water alone.

Taxon
Capsicum annuum L.Capsicum annuumt0
Treatment
coconut water was used synergistically with AgNO3e0
Outcome
production of elongated rooted shootse0
Direction
highere0
Value
47.2%e0
Qualifier
14.4%e0
Comparator
coconut water alonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“there was significantly higher production of elongated rooted shoots when coconut water was used synergistically with AgNO3 (47.2%) as against the coconut water alone (14.4%).”

✓ located verbatim in the source · abstract, characters 1774–1949 · show in text ↑
t0

“Capsicum annuum L.”

✓ located verbatim in the source · title, characters 131–149 · show in text ↑

silver(1+) nitrate · growth · Capsicum annuum (sweet pepper)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.