Abstract
To assess the role of MORC1 in epigenetics in relation to plant immunity, genome-wide chromatin accessibility was compared between mock- or Pseudomonas syringae pv. tomato-inoculated wild type (WT) Arabidopsis, the morc1/2 double mutant, or both. Most changes in chromatin accessibility, scored by DNase I hypersensitive sites (DHSs), were located in the promoters of genes and transposable elements (TEs). Comparisons between morc1/2 and WT receiving the same treatment revealed differential DHSs (dDHSs) predominantly associated with heterochromatic TEs. By contrast, comparisons between mock- and P. syringae pv. tomato-inoculated plants from the same genotype showed dDHSs associated with biotic and abiotic stress-related genes; a smaller but significant population was in TEs. Moreover, many defense genes, including PR-1, PR-2, and PR-5, were proximal to P. syringae pv. tomato-induced, TE-associated dDHSs. A random subset of these defense genes showed moderately delayed or reduced expression or both in P. syringae pv. tomato-infected morc1/2 as compared with WT. MORC1 was physically bound to chromatin in a P. syringae pv. tomato infection-responsive manner at sites dispersed throughout the genome. Notably, silencing of TE-associated dDHSs proximal to these infection-induced, MORC1-interacting sites led to significant suppression of P. syringae pv. tomato-induced transcription of adjacent defense genes, including PR-1. These results provide evidence that MORC1 is associated with TEs and suggest that a subset of these TEs may help regulate their proximal defense genes.
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Claims 8
Claim · model-written summary
Most chromatin accessibility changes occurred in gene and TE promoters.
- Taxon
- Arabidopsis
Arabidopsist0 - Outcome
- changes in chromatin accessibilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most changes in chromatin accessibility, scored by DNase I hypersensitive sites (DHSs), were located in the promoters of genes and transposable elements (TEs)”
t0“Arabidopsis”
Claim · model-written summary
morc1/2 versus wild type showed differential DHSs mainly associated with heterochromatic TEs.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- differential DHSs (dDHSs)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparisons between morc1/2 and WT receiving the same treatment revealed differential DHSs (dDHSs) predominantly associated with heterochromatic TEs”
t0“Arabidopsis”
Claim · model-written summary
Pathogen versus mock comparisons showed differential DHSs associated with stress-related genes.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- P. syringae pv. tomato-inoculatede0
- Interactor
- P. syringae pv. tomato
Pseudomonas syringae pv. tomatoe0pathogen - Outcome
- dDHSs associated with biotic and abiotic stress-related genese0
- Comparator
- mock-e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“comparisons between mock- and P. syringae pv. tomato-inoculated plants from the same genotype showed dDHSs associated with biotic and abiotic stress-related genes”
t0“Arabidopsis”
Claim · model-written summary
A smaller significant population of pathogen-induced differential DHSs was in TEs.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- populatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a smaller but significant population was in TEs”
t0“Arabidopsis”
Claim · model-written summary
Many defense genes were proximal to pathogen-induced TE-associated differential DHSs.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- proximal to P. syringae pv. tomato-induced, TE-associated dDHSse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“many defense genes, including PR-1, PR-2, and PR-5, were proximal to P. syringae pv. tomato-induced, TE-associated dDHSs”
t0“Arabidopsis”
Claim · model-written summary
A subset of defense genes had moderately delayed or reduced expression in infected morc1/2 versus wild type.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- moderately delayed or reduced expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A random subset of these defense genes showed moderately delayed or reduced expression or both in P. syringae pv. tomato-infected morc1/2 as compared with WT”
t0“Arabidopsis”
Claim · model-written summary
MORC1 bound chromatin at dispersed genomic sites in an infection-responsive way.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- MORC1e0
- Outcome
- physically bound to chromatine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MORC1 was physically bound to chromatin in a P. syringae pv. tomato infection-responsive manner at sites dispersed throughout the genome”
t0“Arabidopsis”
Claim · model-written summary
Silencing TE-associated differential DHSs near infection-induced MORC1 sites significantly suppressed pathogen-induced transcription of adjacent defense genes.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- silencing of TE-associated dDHSse0
- Interactor
- P. syringae pv. tomatoPseudomonas syringae pv. tomatoe0pathogen
- Gene
- MORC1e0
- Outcome
- P. syringae pv. tomato-induced transcription of adjacent defense genese0
- Direction
- suppressione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of TE-associated dDHSs proximal to these infection-induced, MORC1-interacting sites led to significant suppression of P. syringae pv. tomato-induced transcription of adjacent defense genes, including PR-1”
t0“Arabidopsis”
The same questions in other papers
loss of function · Pseudomonas syringae pv. tomato · ArabidopsisPositive and negative results
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