Abstract
The avrRpt2 gene from Pseudomonas syringae pv. tomato exhibits avirulence activity on Arabidopsis expressing the resistance gene RPS2 but promotes bacterial virulence on susceptible rps2 Arabidopsis. To understand the functional relationship between the avirulence and virulence activities of avrRpt2, we analyzed a series of six avrRpt2 mutants deficient in eliciting the RPS2-dependent hypersensitive response. We show that the mutants are also severely impaired in triggering RSP2-dependent resistance. Four of these mutants are severely impaired in their virulence activity, whereas two alleles, encoding C-terminal deletions of AvrRpt2, retain significant but slightly reduced virulence activity. Thus, the avirulence and virulence activities of avrRpt2 can be genetically uncoupled. We tested the ability of the two C-terminal deletion mutants to trigger AvrRpt2-induced elimination of the Arabidopsis RIN4 protein and show that they retain this activity but are less efficient than wild-type AvrRpt2. Thus, reduced AvrRpt2 virulence activity is correlated with reduced efficiency in the induction of RIN4 disappearance. This suggests that an alteration in kinetics of RIN4 disappearance triggered by the C-terminal deletion mutants may provide the mechanistic basis for the uncoupling of the avirulence and virulence activities of avrRpt2.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Six avrRpt2 mutants severely impaired the triggering of RPS2-dependent resistance in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- avrRpt2 mutantse1
- Interactor
- Pseudomonas syringae pv. tomato
Pseudomonas syringae pv. tomatoe0pathogen - Gene
- avrRpt2e1
- Outcome
- RSP2-dependent resistancee2
- Direction
- severely impairede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The avrRpt2 gene from Pseudomonas syringae pv. tomato exhibits avirulence activity on Arabidopsis expressing the resistance gene RPS2 but promotes bacterial virulence on susceptible rps2 Arabidopsis”
e1“we analyzed a series of six avrRpt2 mutants deficient in eliciting the RPS2-dependent hypersensitive response”
e2“We show that the mutants are also severely impaired in triggering RSP2-dependent resistance”
Claim · model-written summary
Four avrRpt2 mutants were severely impaired in virulence activity on Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- avrRpt2 mutantse1
- Interactor
- Pseudomonas syringae pv. tomatoPseudomonas syringae pv. tomatoe0pathogen
- Gene
- avrRpt2e1
- Outcome
- virulence activitye3
- Direction
- severely impairede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The avrRpt2 gene from Pseudomonas syringae pv. tomato exhibits avirulence activity on Arabidopsis expressing the resistance gene RPS2 but promotes bacterial virulence on susceptible rps2 Arabidopsis”
e1“we analyzed a series of six avrRpt2 mutants deficient in eliciting the RPS2-dependent hypersensitive response”
e3“Four of these mutants are severely impaired in their virulence activity”
Claim · model-written summary
Two C-terminal deletion alleles of AvrRpt2 retained significant but slightly reduced virulence activity on Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- C-terminal deletions of AvrRpt2e4
- Interactor
- Pseudomonas syringae pv. tomatoPseudomonas syringae pv. tomatoe0pathogen
- Gene
- AvrRpt2e4
- Outcome
- virulence activitye4
- Direction
- slightly reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The avrRpt2 gene from Pseudomonas syringae pv. tomato exhibits avirulence activity on Arabidopsis expressing the resistance gene RPS2 but promotes bacterial virulence on susceptible rps2 Arabidopsis”
e4“whereas two alleles, encoding C-terminal deletions of AvrRpt2, retain significant but slightly reduced virulence activity”
Claim · model-written summary
The avirulence and virulence activities of avrRpt2 can be genetically uncoupled.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- avrRpt2e5
- Outcome
- avirulence and virulence activities of avrRpt2e5
- Qualifier
- can be genetically uncouplede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The avrRpt2 gene from Pseudomonas syringae pv. tomato exhibits avirulence activity on Arabidopsis expressing the resistance gene RPS2 but promotes bacterial virulence on susceptible rps2 Arabidopsis”
e5“Thus, the avirulence and virulence activities of avrRpt2 can be genetically uncoupled”
Claim · model-written summary
The two C-terminal deletion mutants retained AvrRpt2-induced elimination of the Arabidopsis RIN4 protein.
- Taxon
- ArabidopsisArabidopsise6
- Treatment
- C-terminal deletion mutantse6
- Gene
- RIN4e6
- Outcome
- AvrRpt2-induced elimination of the Arabidopsis RIN4 proteine6
- Qualifier
- retain this activitye6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“We tested the ability of the two C-terminal deletion mutants to trigger AvrRpt2-induced elimination of the Arabidopsis RIN4 protein and show that they retain this activity but are less efficient than wild-type AvrRpt2”
Claim · model-written summary
The two C-terminal deletion mutants induced RIN4 elimination less efficiently than wild-type AvrRpt2.
- Taxon
- ArabidopsisArabidopsise6
- Treatment
- C-terminal deletion mutantse6
- Gene
- RIN4e6
- Outcome
- AvrRpt2-induced elimination of the Arabidopsis RIN4 proteine6
- Direction
- less efficiente6
- Comparator
- wild-type AvrRpt2e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“We tested the ability of the two C-terminal deletion mutants to trigger AvrRpt2-induced elimination of the Arabidopsis RIN4 protein and show that they retain this activity but are less efficient than wild-type AvrRpt2”
Claim · model-written summary
Reduced AvrRpt2 virulence activity is correlated with reduced efficiency in inducing RIN4 disappearance in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- AvrRpt2e7
- Outcome
- efficiency in the induction of RIN4 disappearancee7
- Direction
- reducede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The avrRpt2 gene from Pseudomonas syringae pv. tomato exhibits avirulence activity on Arabidopsis expressing the resistance gene RPS2 but promotes bacterial virulence on susceptible rps2 Arabidopsis”
e7“Thus, reduced AvrRpt2 virulence activity is correlated with reduced efficiency in the induction of RIN4 disappearance”
The same questions in other papers
loss of function · Pseudomonas syringae pv. tomato · ArabidopsisPositive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.