Abstract
Soil salinity is a major abiotic stress and salt overly sensitive (SOS) pathway plays an important role in imparting tolerance to salinity by reinstating cellular ionic equilibrium. Salt overly sensitive 1 (SOS1) gene of SOS pathway has been implicated in increasing salt tolerance in plants. In this study, a 734 bp fragment of SOS1 promoter (SbUSOS1) was isolated from a halophyte Salicornia brachiata Roxb. In silico analysis of SbUSOS1 predicted several cis-acting regulatory elements such as DOF motif, GT elements, ABRE-like sequence, and root specific motifs. Functional validation of SbUSOS1 into tobacco stems and leaves using the GUS reporter gene showed that this promoter is induced by salt stress (250 mM NaCl) but not by ABA (500 μM) and cold (4 °C) stresses. This study indicated that SbUSOS1 was functional with predicted cis-acting elements that could be responsible for its salt-inducible nature. It can be used for the development of salt stress tolerant transgenic plants.
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Claims 5
Claim · model-written summary
The SbUSOS1 promoter is induced by salt stress (250 mM NaCl) in tobacco.
- Taxon
- tobacco
Nicotiana tabacume2 - Treatment
- salt stress (250 mM NaCl)e1
- Gene
- SbUSOS1e2
- Outcome
- GUS reporter genee2
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“showed that this promoter is induced by salt stress (250 mM NaCl) but not by ABA (500 μM) and cold”
e2“Functional validation of SbUSOS1 into tobacco stems and leaves using the GUS reporter gene”
Claim · model-written summary
The SbUSOS1 promoter is not induced by ABA (500 μM) in tobacco.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- ABA (500 μM)e1
- Gene
- SbUSOS1e2
- Outcome
- GUS reporter genee2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“showed that this promoter is induced by salt stress (250 mM NaCl) but not by ABA (500 μM) and cold”
e2“Functional validation of SbUSOS1 into tobacco stems and leaves using the GUS reporter gene”
Claim · model-written summary
The SbUSOS1 promoter is not induced by cold in tobacco.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- colde1
- Gene
- SbUSOS1e2
- Outcome
- GUS reporter genee2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“showed that this promoter is induced by salt stress (250 mM NaCl) but not by ABA (500 μM) and cold”
e2“Functional validation of SbUSOS1 into tobacco stems and leaves using the GUS reporter gene”
Claim · model-written summary
The SbUSOS1 promoter contains several predicted cis-acting regulatory elements.
- Taxon
- Salicornia brachiataSalicornia brachiatae4
- Gene
- SbUSOS1e3
- Outcome
- cis-acting regulatory elementse3
Verbatim
e3“In silico analysis of SbUSOS1 predicted several cis-acting regulatory elements such as DOF motif, GT elements, ABRE-like sequence, and root specific motifs.”
e4“a 734 bp fragment of SOS1 promoter (SbUSOS1) was isolated from a halophyte Salicornia brachiata Roxb.”
Claim · model-written summary
The predicted cis-acting elements may explain the salt-inducible nature of the SbUSOS1 promoter.
- Taxon
- Salicornia brachiataSalicornia brachiatae4
- Outcome
- salt-inducible naturee5
- Qualifier
- predicted cis-acting elementse5
Verbatim
e5“predicted cis-acting elements that could be responsible for its salt-inducible nature”
e4“a 734 bp fragment of SOS1 promoter (SbUSOS1) was isolated from a halophyte Salicornia brachiata Roxb.”
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