Abstract
PsSEOF1, a SEO (sieve element occlusion) gene family protein (forisome) is calcium powered motor protein and is located close to plasma membrane of sieve element. In sieve element (SE) it senses the calcium ion levels and undergoes ATP-independent conformational shifts. Forisome, meaning gate-bodies (Latin foris: wing of a gate; Greek soma: body). Recent reports show that SEO gene family protein can prevent the loss of nutrient rich photoassimilate upon wound injury. The regulation of SEO protein forisome under abiotic/ biotic stress is still unknown. The analysis of cis-regulatory element present in the upstream region is not well understood. Tissue specific promoters guarantee correct expression when it perceives particular stimuli. Here we report isolation of tissue specific promoter of PsSEOF1 was isolated by gene walking PCR from P. sativum (pea) genomic DNA library constructed by BD genome walker kit. In silico analysis revealed several putative cis element within this promoter sequence like wound response, cold, dehydration. Putative elements which might be required for its vascular tissue specificity has also been identified. The GUS activities of PsSEOF1 promoter-GUS chimeric construct in the agroinfiltrated leaves under different environmental stress abiotic and biotic like wound, cold, salt and phytohormones has shown high level of GUS activity. To identify the activity of PsSEOF1 promoter under different stress condition an Agrobacterium-mediated transient expression of tobacco plants were subjected to histochemical GUS staining. Stress-inducible nature of PsSEOF1 promoter opens possibility for the study of the PsSEOF1 gene regulation under stress condition. The isolated promoter sequence could serve as an important candidate for tissue specific promoter in genetic engineering of plant under stress conditions.
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Claims 4
Claim · model-written summary
A tissue-specific PsSEOF1 promoter was isolated from pea genomic DNA.
Verbatim
e0“tissue specific promoter of PsSEOF1 was isolated by gene walking PCR from P. sativum (pea) genomic DNA library”
Claim · model-written summary
The promoter had putative wound, cold and dehydration cis elements.
Verbatim
e0“In silico analysis revealed several putative cis element within this promoter sequence like wound response, cold, dehydration”
t0“PsSEOF1gene fromPisum sativum”
e1“P. sativum (pea)”
Claim · model-written summary
Putative elements for vascular tissue specificity were identified.
Verbatim
e0“Putative elements which might be required for its vascular tissue specificity has also been identified”
t0“P. sativum (pea)”
t1“promoter of PsSEOF1 was isolated by gene walking PCR”
Claim · model-written summary
PsSEOF1 promoter constructs showed high GUS activity under environmental stresses.
- Taxon
- tobacco plants
Nicotiana tabacumt0 - Organ / stage
- leavese0
- Treatment
- wound, cold, salt and phytohormonese0
- Gene
- PsSEOF1e0
- Outcome
- GUS activitye0
- Direction
- high levele0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GUS activities of PsSEOF1 promoter-GUS chimeric construct in the agroinfiltrated leaves under different environmental stress abiotic and biotic like wound, cold, salt and phytohormones has shown high level of GUS activity”
t0“tobacco plants”
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