Abstract
Studies were conducted to determine the efficacy and mechanism of 1-methylcyclopropene (1-MCP) and ethephon on softening of Ailsa Craig tomato fruit. Ethylene production, reduction of fruit firmness and protopectin content were suppressed in fruit exposed to 1.0 µL/L 1-MCP. Comparing with control fruit, the climacteric ethylene peak was delayed by approximately 12 d and polygalacturonase activity was strongly inhibited in 1.0 µL/L 1-MCP treated fruit. On the other hand, ethylene production, reduction of fruit firmness, protopectin content and polygalacturonase activity of 100 µL/L ethephon treated fruit were strongly promoted compared with these indexes of untreated fruit. Moreover, electrophoretic mobility shift assay (EMSA) and Chromatin Immunoprecipitation (ChIP) assay proved that the expression of PG and tomato fruit softening was regulated by the transcription activator RIN. Practical Applications The RIN transcription factor plays an indispensable role in fruit ripening and softening. According to the physiological index of tomato fruit under varied concentrations of 1-MCP and ethephon, optimal concentrations of 1-MCP and ethephon would be used for further studies and industrial applications. Furthermore our study clearly showed RIN transcription factor directly regulated the expression of PG. Since the importance of polygalacturonase (PG) activity and indispensable role of RIN during the process of fruit softening, it suggested that RIN affects tomato fruit softening via directly acting on PG. It provides new clues to understand the molecular mechanism of fruit softening. To sum, these results will help to further clarify the mechanism of tomato fruit softening, and to maintain the quality and prolong the shelf life of tomato fruit.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
1-MCP suppressed ethylene production in Ailsa Craig tomato fruit.
- Taxon
- Tomato
Solanum lycopersicumt0 - Material
- Ailsa Craigt0
- Treatment
- 1.0 µL/L 1-MCPe0
- Outcome
- Ethylene productione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ethylene production, reduction of fruit firmness and protopectin content were suppressed in fruit exposed to 1.0 µL/L 1-MCP”
t0“Ailsa Craig Tomato Fruit”
Claim · model-written summary
1-MCP suppressed the reduction of fruit firmness and protopectin content.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Ailsa Craigt0
- Treatment
- 1.0 µL/L 1-MCPe0
- Outcome
- reduction of fruit firmness and protopectin contente0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduction of fruit firmness and protopectin content were suppressed in fruit exposed to 1.0 µL/L 1-MCP”
t0“Ailsa Craig Tomato Fruit”
Claim · model-written summary
1-MCP delayed the climacteric ethylene peak approximately 12 days versus controls.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Ailsa Craigt0
- Treatment
- 1.0 µL/L 1-MCPe0
- Outcome
- climacteric ethylene peake0
- Direction
- delayede0
- Value
- approximately 12 de0
- Comparator
- control fruite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparing with control fruit, the climacteric ethylene peak was delayed by approximately 12 d and polygalacturonase activity was strongly inhibited in 1.0 µL/L 1-MCP treated fruit”
t0“Ailsa Craig Tomato Fruit”
Claim · model-written summary
1-MCP strongly inhibited polygalacturonase activity versus controls.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Ailsa Craigt0
- Treatment
- 1.0 µL/L 1-MCPe0
- Outcome
- polygalacturonase activitye0
- Direction
- inhibitede0
- Comparator
- control fruite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparing with control fruit, the climacteric ethylene peak was delayed by approximately 12 d and polygalacturonase activity was strongly inhibited in 1.0 µL/L 1-MCP treated fruit”
t0“Ailsa Craig Tomato Fruit”
Claim · model-written summary
Ethephon promoted ethylene production, firmness reduction and protopectin content versus untreated fruit.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Ailsa Craigt0
- Treatment
- 100 µL/L ethephone0
- Outcome
- ethylene production, reduction of fruit firmness, protopectin contente0
- Direction
- promotede0
- Comparator
- untreated fruite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene production, reduction of fruit firmness, protopectin content and polygalacturonase activity of 100 µL/L ethephon treated fruit were strongly promoted compared with these indexes of untreated fruit”
t0“Ailsa Craig Tomato Fruit”
Claim · model-written summary
Ethephon promoted polygalacturonase activity versus untreated fruit.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Ailsa Craigt0
- Treatment
- 100 µL/L ethephone0
- Outcome
- polygalacturonase activitye0
- Direction
- promotede0
- Comparator
- untreated fruite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“polygalacturonase activity of 100 µL/L ethephon treated fruit were strongly promoted compared with these indexes of untreated fruit”
t0“Ailsa Craig Tomato Fruit”
Claim · model-written summary
EMSA and ChIP indicated RIN regulates PG expression.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- RINe0
- Outcome
- expression of PGe0
- Direction
- regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“electrophoretic mobility shift assay (EMSA) and Chromatin Immunoprecipitation (ChIP) assay proved that the expression of PG and tomato fruit softening was regulated by the transcription activator RIN”
Claim · model-written summary
EMSA and ChIP indicated RIN regulates tomato fruit softening.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- RINe0
- Outcome
- tomato fruit softeninge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“electrophoretic mobility shift assay (EMSA) and Chromatin Immunoprecipitation (ChIP) assay proved that the expression of PG and tomato fruit softening was regulated by the transcription activator RIN”
Claim · model-written summary
The authors suggest RIN affects tomato softening through direct action on PG.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- RINe0
- Outcome
- tomato fruit softeninge0
- Qualifier
- directly acting on PGe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it suggested that RIN affects tomato fruit softening via directly acting on PG”
The same questions in other papers
1-methylcyclopropene · physiology: hormones · Solanum lycopersicum (tomato)Results differ
19 papers in the same direction as this paper · 3 in the opposite direction
- positive
- negative
- no effect
19 papers more in this group
See all 23 papers →(2-chloroethyl)phosphonic acid · physiology: enzyme activity · Solanum lycopersicum (tomato)Positive and negative results
2 in the opposite direction
- positive
- positive
1-methylcyclopropene · physiology: enzyme activity · Solanum lycopersicum (tomato)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
(2-chloroethyl)phosphonic acid · physiology: hormones · Solanum lycopersicum (tomato)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
1-methylcyclopropene · quality: postharvest · Solanum lycopersicum (tomato)Results agree
52 papers in the same direction as this paper
- positive
- positive
- positive
49 papers more in this group
See all 53 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.